Cargando…

Efficient generation of transgenic cattle using the DNA transposon and their analysis by next-generation sequencing

Here, we efficiently generated transgenic cattle using two transposon systems (Sleeping Beauty and Piggybac) and their genomes were analyzed by next-generation sequencing (NGS). Blastocysts derived from microinjection of DNA transposons were selected and transferred into recipient cows. Nine transge...

Descripción completa

Detalles Bibliográficos
Autores principales: Yum, Soo-Young, Lee, Song-Jeon, Kim, Hyun-Min, Choi, Woo-Jae, Park, Ji-Hyun, Lee, Won-Wu, Kim, Hee-Soo, Kim, Hyeong-Jong, Bae, Seong-Hun, Lee, Je-Hyeong, Moon, Joo-Yeong, Lee, Ji-Hyun, Lee, Choong-Il, Son, Bong-Jun, Song, Sang-Hoon, Ji, Su-Min, Kim, Seong-Jin, Jang, Goo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4914850/
https://www.ncbi.nlm.nih.gov/pubmed/27324781
http://dx.doi.org/10.1038/srep27185
Descripción
Sumario:Here, we efficiently generated transgenic cattle using two transposon systems (Sleeping Beauty and Piggybac) and their genomes were analyzed by next-generation sequencing (NGS). Blastocysts derived from microinjection of DNA transposons were selected and transferred into recipient cows. Nine transgenic cattle have been generated and grown-up to date without any health issues except two. Some of them expressed strong fluorescence and the transgene in the oocytes from a superovulating one were detected by PCR and sequencing. To investigate genomic variants by the transgene transposition, whole genomic DNA were analyzed by NGS. We found that preferred transposable integration (TA or TTAA) was identified in their genome. Even though multi-copies (i.e. fifteen) were confirmed, there was no significant difference in genome instabilities. In conclusion, we demonstrated that transgenic cattle using the DNA transposon system could be efficiently generated, and all those animals could be a valuable resource for agriculture and veterinary science.