Cargando…
The use of platelet-rich fibrin combined with periodontal ligament and jaw bone mesenchymal stem cell sheets for periodontal tissue engineering
Periodontal regeneration involves the restoration of at least three unique tissues: cementum, periodontal ligament tissue (PDL) and alveolar bone tissue. Here, we first isolated human PDL stem cells (PDLSCs) and jaw bone mesenchymal stem cells (JBMSCs). These cells were then induced to form cell she...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4914939/ https://www.ncbi.nlm.nih.gov/pubmed/27324079 http://dx.doi.org/10.1038/srep28126 |
_version_ | 1782438610788679680 |
---|---|
author | Wang, Zhong-Shan Feng, Zhi-Hong Wu, Guo-Feng Bai, Shi-Zhu Dong, Yan Chen, Fa-Ming Zhao, Yi-Min |
author_facet | Wang, Zhong-Shan Feng, Zhi-Hong Wu, Guo-Feng Bai, Shi-Zhu Dong, Yan Chen, Fa-Ming Zhao, Yi-Min |
author_sort | Wang, Zhong-Shan |
collection | PubMed |
description | Periodontal regeneration involves the restoration of at least three unique tissues: cementum, periodontal ligament tissue (PDL) and alveolar bone tissue. Here, we first isolated human PDL stem cells (PDLSCs) and jaw bone mesenchymal stem cells (JBMSCs). These cells were then induced to form cell sheets using an ascorbic acid-rich approach, and the cell sheet properties, including morphology, thickness and gene expression profile, were compared. Platelet-rich fibrin (PRF) derived from human venous blood was then fabricated into bioabsorbable fibrin scaffolds containing various growth factors. Finally, the in vivo potential of a cell-material construct based on PDLSC sheets, PRF scaffolds and JBMSC sheets to form periodontal tissue was assessed in a nude mouse model. In this model, PDLSC sheet/PRF/JBMSC sheet composites were placed in a simulated periodontal space comprising human treated dentin matrix (TDM) and hydroxyapatite (HA)/tricalcium phosphate (TCP) frameworks. Eight weeks after implantation, the PDLSC sheets tended to develop into PDL-like tissues, while the JBMSC sheets tended to produce predominantly bone-like tissues. In addition, the PDLSC sheet/PRF/JBMSC sheet composites generated periodontal tissue-like structures containing PDL- and bone-like tissues. Further improvements in this cell transplantation design may have the potential to provide an effective approach for future periodontal tissue regeneration. |
format | Online Article Text |
id | pubmed-4914939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49149392016-06-27 The use of platelet-rich fibrin combined with periodontal ligament and jaw bone mesenchymal stem cell sheets for periodontal tissue engineering Wang, Zhong-Shan Feng, Zhi-Hong Wu, Guo-Feng Bai, Shi-Zhu Dong, Yan Chen, Fa-Ming Zhao, Yi-Min Sci Rep Article Periodontal regeneration involves the restoration of at least three unique tissues: cementum, periodontal ligament tissue (PDL) and alveolar bone tissue. Here, we first isolated human PDL stem cells (PDLSCs) and jaw bone mesenchymal stem cells (JBMSCs). These cells were then induced to form cell sheets using an ascorbic acid-rich approach, and the cell sheet properties, including morphology, thickness and gene expression profile, were compared. Platelet-rich fibrin (PRF) derived from human venous blood was then fabricated into bioabsorbable fibrin scaffolds containing various growth factors. Finally, the in vivo potential of a cell-material construct based on PDLSC sheets, PRF scaffolds and JBMSC sheets to form periodontal tissue was assessed in a nude mouse model. In this model, PDLSC sheet/PRF/JBMSC sheet composites were placed in a simulated periodontal space comprising human treated dentin matrix (TDM) and hydroxyapatite (HA)/tricalcium phosphate (TCP) frameworks. Eight weeks after implantation, the PDLSC sheets tended to develop into PDL-like tissues, while the JBMSC sheets tended to produce predominantly bone-like tissues. In addition, the PDLSC sheet/PRF/JBMSC sheet composites generated periodontal tissue-like structures containing PDL- and bone-like tissues. Further improvements in this cell transplantation design may have the potential to provide an effective approach for future periodontal tissue regeneration. Nature Publishing Group 2016-06-21 /pmc/articles/PMC4914939/ /pubmed/27324079 http://dx.doi.org/10.1038/srep28126 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Wang, Zhong-Shan Feng, Zhi-Hong Wu, Guo-Feng Bai, Shi-Zhu Dong, Yan Chen, Fa-Ming Zhao, Yi-Min The use of platelet-rich fibrin combined with periodontal ligament and jaw bone mesenchymal stem cell sheets for periodontal tissue engineering |
title | The use of platelet-rich fibrin combined with periodontal ligament and jaw bone mesenchymal stem cell sheets for periodontal tissue engineering |
title_full | The use of platelet-rich fibrin combined with periodontal ligament and jaw bone mesenchymal stem cell sheets for periodontal tissue engineering |
title_fullStr | The use of platelet-rich fibrin combined with periodontal ligament and jaw bone mesenchymal stem cell sheets for periodontal tissue engineering |
title_full_unstemmed | The use of platelet-rich fibrin combined with periodontal ligament and jaw bone mesenchymal stem cell sheets for periodontal tissue engineering |
title_short | The use of platelet-rich fibrin combined with periodontal ligament and jaw bone mesenchymal stem cell sheets for periodontal tissue engineering |
title_sort | use of platelet-rich fibrin combined with periodontal ligament and jaw bone mesenchymal stem cell sheets for periodontal tissue engineering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4914939/ https://www.ncbi.nlm.nih.gov/pubmed/27324079 http://dx.doi.org/10.1038/srep28126 |
work_keys_str_mv | AT wangzhongshan theuseofplateletrichfibrincombinedwithperiodontalligamentandjawbonemesenchymalstemcellsheetsforperiodontaltissueengineering AT fengzhihong theuseofplateletrichfibrincombinedwithperiodontalligamentandjawbonemesenchymalstemcellsheetsforperiodontaltissueengineering AT wuguofeng theuseofplateletrichfibrincombinedwithperiodontalligamentandjawbonemesenchymalstemcellsheetsforperiodontaltissueengineering AT baishizhu theuseofplateletrichfibrincombinedwithperiodontalligamentandjawbonemesenchymalstemcellsheetsforperiodontaltissueengineering AT dongyan theuseofplateletrichfibrincombinedwithperiodontalligamentandjawbonemesenchymalstemcellsheetsforperiodontaltissueengineering AT chenfaming theuseofplateletrichfibrincombinedwithperiodontalligamentandjawbonemesenchymalstemcellsheetsforperiodontaltissueengineering AT zhaoyimin theuseofplateletrichfibrincombinedwithperiodontalligamentandjawbonemesenchymalstemcellsheetsforperiodontaltissueengineering AT wangzhongshan useofplateletrichfibrincombinedwithperiodontalligamentandjawbonemesenchymalstemcellsheetsforperiodontaltissueengineering AT fengzhihong useofplateletrichfibrincombinedwithperiodontalligamentandjawbonemesenchymalstemcellsheetsforperiodontaltissueengineering AT wuguofeng useofplateletrichfibrincombinedwithperiodontalligamentandjawbonemesenchymalstemcellsheetsforperiodontaltissueengineering AT baishizhu useofplateletrichfibrincombinedwithperiodontalligamentandjawbonemesenchymalstemcellsheetsforperiodontaltissueengineering AT dongyan useofplateletrichfibrincombinedwithperiodontalligamentandjawbonemesenchymalstemcellsheetsforperiodontaltissueengineering AT chenfaming useofplateletrichfibrincombinedwithperiodontalligamentandjawbonemesenchymalstemcellsheetsforperiodontaltissueengineering AT zhaoyimin useofplateletrichfibrincombinedwithperiodontalligamentandjawbonemesenchymalstemcellsheetsforperiodontaltissueengineering |