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Developmental and stress regulation on expression of a novel miRNA, Fan-miR73, and its target ABI5 in strawberry
Abscisic acid (ABA) is a critical plant hormone for fruit ripening and adaptive stress responses in strawberry. Previous high-throughput sequencing results indicated that ABA-insensitive (ABI)5, an important transcription factor in the ABA signaling pathway, was a target for a novel microRNA (miRNA)...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4914977/ https://www.ncbi.nlm.nih.gov/pubmed/27325048 http://dx.doi.org/10.1038/srep28385 |
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author | Li, Dongdong Mou, Wangshu Luo, Zisheng Li, Li Limwachiranon, Jarukitt Mao, Linchun Ying, Tiejin |
author_facet | Li, Dongdong Mou, Wangshu Luo, Zisheng Li, Li Limwachiranon, Jarukitt Mao, Linchun Ying, Tiejin |
author_sort | Li, Dongdong |
collection | PubMed |
description | Abscisic acid (ABA) is a critical plant hormone for fruit ripening and adaptive stress responses in strawberry. Previous high-throughput sequencing results indicated that ABA-insensitive (ABI)5, an important transcription factor in the ABA signaling pathway, was a target for a novel microRNA (miRNA), Fan-miR73. In the present study, exogenous ABA treatment was found to accelerate fruit ripening through differentially regulating the transcripts of ABA metabolism and signal transduction related genes, including NCED1, PYR1, ABI1, and SnRK2.2. Expression of Fan-miR73 was down-regulated in response to exogenous ABA treatment in a dosage-dependent manner, which resulted in an accumulation of ABI5 transcripts in the ripening-accelerated fruits. In addition, both UV-B radiation and salinity stress reduced the transcript levels of Fan-miR73, whereas promoted ABI5 expression. Furthermore, high negative correlations between the transcriptional abundance of Fan-miR73 and ABI5 were observed during ripening and in response to stress stimuli. These results enriched the possible regulatory role of miRNA involved in the post-transcriptional modification of ABI5 during strawberry ripening, as well as responses to environmental stresses. |
format | Online Article Text |
id | pubmed-4914977 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49149772016-06-27 Developmental and stress regulation on expression of a novel miRNA, Fan-miR73, and its target ABI5 in strawberry Li, Dongdong Mou, Wangshu Luo, Zisheng Li, Li Limwachiranon, Jarukitt Mao, Linchun Ying, Tiejin Sci Rep Article Abscisic acid (ABA) is a critical plant hormone for fruit ripening and adaptive stress responses in strawberry. Previous high-throughput sequencing results indicated that ABA-insensitive (ABI)5, an important transcription factor in the ABA signaling pathway, was a target for a novel microRNA (miRNA), Fan-miR73. In the present study, exogenous ABA treatment was found to accelerate fruit ripening through differentially regulating the transcripts of ABA metabolism and signal transduction related genes, including NCED1, PYR1, ABI1, and SnRK2.2. Expression of Fan-miR73 was down-regulated in response to exogenous ABA treatment in a dosage-dependent manner, which resulted in an accumulation of ABI5 transcripts in the ripening-accelerated fruits. In addition, both UV-B radiation and salinity stress reduced the transcript levels of Fan-miR73, whereas promoted ABI5 expression. Furthermore, high negative correlations between the transcriptional abundance of Fan-miR73 and ABI5 were observed during ripening and in response to stress stimuli. These results enriched the possible regulatory role of miRNA involved in the post-transcriptional modification of ABI5 during strawberry ripening, as well as responses to environmental stresses. Nature Publishing Group 2016-06-21 /pmc/articles/PMC4914977/ /pubmed/27325048 http://dx.doi.org/10.1038/srep28385 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Li, Dongdong Mou, Wangshu Luo, Zisheng Li, Li Limwachiranon, Jarukitt Mao, Linchun Ying, Tiejin Developmental and stress regulation on expression of a novel miRNA, Fan-miR73, and its target ABI5 in strawberry |
title | Developmental and stress regulation on expression of a novel miRNA, Fan-miR73, and its target ABI5 in strawberry |
title_full | Developmental and stress regulation on expression of a novel miRNA, Fan-miR73, and its target ABI5 in strawberry |
title_fullStr | Developmental and stress regulation on expression of a novel miRNA, Fan-miR73, and its target ABI5 in strawberry |
title_full_unstemmed | Developmental and stress regulation on expression of a novel miRNA, Fan-miR73, and its target ABI5 in strawberry |
title_short | Developmental and stress regulation on expression of a novel miRNA, Fan-miR73, and its target ABI5 in strawberry |
title_sort | developmental and stress regulation on expression of a novel mirna, fan-mir73, and its target abi5 in strawberry |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4914977/ https://www.ncbi.nlm.nih.gov/pubmed/27325048 http://dx.doi.org/10.1038/srep28385 |
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