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Developmental and stress regulation on expression of a novel miRNA, Fan-miR73, and its target ABI5 in strawberry

Abscisic acid (ABA) is a critical plant hormone for fruit ripening and adaptive stress responses in strawberry. Previous high-throughput sequencing results indicated that ABA-insensitive (ABI)5, an important transcription factor in the ABA signaling pathway, was a target for a novel microRNA (miRNA)...

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Autores principales: Li, Dongdong, Mou, Wangshu, Luo, Zisheng, Li, Li, Limwachiranon, Jarukitt, Mao, Linchun, Ying, Tiejin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4914977/
https://www.ncbi.nlm.nih.gov/pubmed/27325048
http://dx.doi.org/10.1038/srep28385
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author Li, Dongdong
Mou, Wangshu
Luo, Zisheng
Li, Li
Limwachiranon, Jarukitt
Mao, Linchun
Ying, Tiejin
author_facet Li, Dongdong
Mou, Wangshu
Luo, Zisheng
Li, Li
Limwachiranon, Jarukitt
Mao, Linchun
Ying, Tiejin
author_sort Li, Dongdong
collection PubMed
description Abscisic acid (ABA) is a critical plant hormone for fruit ripening and adaptive stress responses in strawberry. Previous high-throughput sequencing results indicated that ABA-insensitive (ABI)5, an important transcription factor in the ABA signaling pathway, was a target for a novel microRNA (miRNA), Fan-miR73. In the present study, exogenous ABA treatment was found to accelerate fruit ripening through differentially regulating the transcripts of ABA metabolism and signal transduction related genes, including NCED1, PYR1, ABI1, and SnRK2.2. Expression of Fan-miR73 was down-regulated in response to exogenous ABA treatment in a dosage-dependent manner, which resulted in an accumulation of ABI5 transcripts in the ripening-accelerated fruits. In addition, both UV-B radiation and salinity stress reduced the transcript levels of Fan-miR73, whereas promoted ABI5 expression. Furthermore, high negative correlations between the transcriptional abundance of Fan-miR73 and ABI5 were observed during ripening and in response to stress stimuli. These results enriched the possible regulatory role of miRNA involved in the post-transcriptional modification of ABI5 during strawberry ripening, as well as responses to environmental stresses.
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spelling pubmed-49149772016-06-27 Developmental and stress regulation on expression of a novel miRNA, Fan-miR73, and its target ABI5 in strawberry Li, Dongdong Mou, Wangshu Luo, Zisheng Li, Li Limwachiranon, Jarukitt Mao, Linchun Ying, Tiejin Sci Rep Article Abscisic acid (ABA) is a critical plant hormone for fruit ripening and adaptive stress responses in strawberry. Previous high-throughput sequencing results indicated that ABA-insensitive (ABI)5, an important transcription factor in the ABA signaling pathway, was a target for a novel microRNA (miRNA), Fan-miR73. In the present study, exogenous ABA treatment was found to accelerate fruit ripening through differentially regulating the transcripts of ABA metabolism and signal transduction related genes, including NCED1, PYR1, ABI1, and SnRK2.2. Expression of Fan-miR73 was down-regulated in response to exogenous ABA treatment in a dosage-dependent manner, which resulted in an accumulation of ABI5 transcripts in the ripening-accelerated fruits. In addition, both UV-B radiation and salinity stress reduced the transcript levels of Fan-miR73, whereas promoted ABI5 expression. Furthermore, high negative correlations between the transcriptional abundance of Fan-miR73 and ABI5 were observed during ripening and in response to stress stimuli. These results enriched the possible regulatory role of miRNA involved in the post-transcriptional modification of ABI5 during strawberry ripening, as well as responses to environmental stresses. Nature Publishing Group 2016-06-21 /pmc/articles/PMC4914977/ /pubmed/27325048 http://dx.doi.org/10.1038/srep28385 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Li, Dongdong
Mou, Wangshu
Luo, Zisheng
Li, Li
Limwachiranon, Jarukitt
Mao, Linchun
Ying, Tiejin
Developmental and stress regulation on expression of a novel miRNA, Fan-miR73, and its target ABI5 in strawberry
title Developmental and stress regulation on expression of a novel miRNA, Fan-miR73, and its target ABI5 in strawberry
title_full Developmental and stress regulation on expression of a novel miRNA, Fan-miR73, and its target ABI5 in strawberry
title_fullStr Developmental and stress regulation on expression of a novel miRNA, Fan-miR73, and its target ABI5 in strawberry
title_full_unstemmed Developmental and stress regulation on expression of a novel miRNA, Fan-miR73, and its target ABI5 in strawberry
title_short Developmental and stress regulation on expression of a novel miRNA, Fan-miR73, and its target ABI5 in strawberry
title_sort developmental and stress regulation on expression of a novel mirna, fan-mir73, and its target abi5 in strawberry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4914977/
https://www.ncbi.nlm.nih.gov/pubmed/27325048
http://dx.doi.org/10.1038/srep28385
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