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Adjusting microbiome profiles for differences in microbial load by spike-in bacteria

BACKGROUND: Next-generation 16S ribosomal RNA gene sequencing is widely used to determine the relative composition of the mammalian gut microbiomes. However, in the absence of a reference, this does not reveal alterations in absolute abundance of specific operational taxonomic units if microbial loa...

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Detalles Bibliográficos
Autores principales:	Stämmler, Frank, Gläsner, Joachim, Hiergeist, Andreas, Holler, Ernst, Weber, Daniela, Oefner, Peter J., Gessner, André, Spang, Rainer
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2016
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4915049/ https://www.ncbi.nlm.nih.gov/pubmed/27329048 http://dx.doi.org/10.1186/s40168-016-0175-0

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