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Structural and dynamic views of the CRISPR-Cas system at the single-molecule level

The CRISPR-Cas system has emerged as a fascinating and important genome editing tool. It is now widely used in biology, biotechnology, and biomedical research in both academic and industrial settings. To improve the specificity and efficiency of Cas nucleases and to extend the applications of these...

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Detalles Bibliográficos
Autores principales: Lee, Seung Hwan, Bae, Sangsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4915238/
https://www.ncbi.nlm.nih.gov/pubmed/26923305
http://dx.doi.org/10.5483/BMBRep.2016.49.4.042
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author Lee, Seung Hwan
Bae, Sangsu
author_facet Lee, Seung Hwan
Bae, Sangsu
author_sort Lee, Seung Hwan
collection PubMed
description The CRISPR-Cas system has emerged as a fascinating and important genome editing tool. It is now widely used in biology, biotechnology, and biomedical research in both academic and industrial settings. To improve the specificity and efficiency of Cas nucleases and to extend the applications of these systems for other areas of research, an understanding of their precise working mechanisms is crucial. In this review, we summarize current studies on the molecular structures and dynamic functions of type I and type II Cas nucleases, with a focus on target DNA searching and cleavage processes as revealed by single-molecule observations. [BMB Reports 2016; 49(4): 201-207]
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spelling pubmed-49152382016-06-23 Structural and dynamic views of the CRISPR-Cas system at the single-molecule level Lee, Seung Hwan Bae, Sangsu BMB Rep Invited Mini Review The CRISPR-Cas system has emerged as a fascinating and important genome editing tool. It is now widely used in biology, biotechnology, and biomedical research in both academic and industrial settings. To improve the specificity and efficiency of Cas nucleases and to extend the applications of these systems for other areas of research, an understanding of their precise working mechanisms is crucial. In this review, we summarize current studies on the molecular structures and dynamic functions of type I and type II Cas nucleases, with a focus on target DNA searching and cleavage processes as revealed by single-molecule observations. [BMB Reports 2016; 49(4): 201-207] Korean Society for Biochemistry and Molecular Biology 2016-04-30 /pmc/articles/PMC4915238/ /pubmed/26923305 http://dx.doi.org/10.5483/BMBRep.2016.49.4.042 Text en Copyright © 2016, Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Invited Mini Review
Lee, Seung Hwan
Bae, Sangsu
Structural and dynamic views of the CRISPR-Cas system at the single-molecule level
title Structural and dynamic views of the CRISPR-Cas system at the single-molecule level
title_full Structural and dynamic views of the CRISPR-Cas system at the single-molecule level
title_fullStr Structural and dynamic views of the CRISPR-Cas system at the single-molecule level
title_full_unstemmed Structural and dynamic views of the CRISPR-Cas system at the single-molecule level
title_short Structural and dynamic views of the CRISPR-Cas system at the single-molecule level
title_sort structural and dynamic views of the crispr-cas system at the single-molecule level
topic Invited Mini Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4915238/
https://www.ncbi.nlm.nih.gov/pubmed/26923305
http://dx.doi.org/10.5483/BMBRep.2016.49.4.042
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