Sub-angstrom single-molecule measurements of motor proteins using a nanopore

Present techniques for measuring the motion of single motor proteins, such as FRET and optical tweezers, are limited to a resolution of ~300 pm. We use ion current modulation through the protein nanopore MspA to observe translocation of helicase Hel308 on DNA with up to ~40 picometer sensitivity. Th...

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Detalles Bibliográficos
Autores principales: Derrington, Ian M, Craig, Jonathan M, Stava, Eric, Laszlo, Andrew H, Ross, Brian C, Brinkerhoff, Henry, Nova, Ian C, Doering, Kenji, Tickman, Benjamin I, Ronaghi, Mostafa, Mandell, Jeffrey G, Gunderson, Kevin L, Gundlach, Jens H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4915380/
https://www.ncbi.nlm.nih.gov/pubmed/26414351
http://dx.doi.org/10.1038/nbt.3357
Descripción
Sumario:Present techniques for measuring the motion of single motor proteins, such as FRET and optical tweezers, are limited to a resolution of ~300 pm. We use ion current modulation through the protein nanopore MspA to observe translocation of helicase Hel308 on DNA with up to ~40 picometer sensitivity. This approach should be applicable to any protein that translocates on DNA or RNA, including helicases, polymerases, recombinases and DNA repair enzymes.

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