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Twenty-Five New Viruses Associated with the Drosophilidae (Diptera)
Drosophila melanogaster is an important laboratory model for studies of antiviral immunity in invertebrates, and Drosophila species provide a valuable system to study virus host range and host switching. Here, we use metagenomic RNA sequencing of about 1600 adult flies to discover 25 new RNA viruses...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Libertas Academica
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4915790/ https://www.ncbi.nlm.nih.gov/pubmed/27375356 http://dx.doi.org/10.4137/EBO.S39454 |
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author | Webster, Claire L. Longdon, Ben Lewis, Samuel H. Obbard, Darren J. |
author_facet | Webster, Claire L. Longdon, Ben Lewis, Samuel H. Obbard, Darren J. |
author_sort | Webster, Claire L. |
collection | PubMed |
description | Drosophila melanogaster is an important laboratory model for studies of antiviral immunity in invertebrates, and Drosophila species provide a valuable system to study virus host range and host switching. Here, we use metagenomic RNA sequencing of about 1600 adult flies to discover 25 new RNA viruses associated with six different drosophilid hosts in the wild. We also provide a comprehensive listing of viruses previously reported from the Drosophilidae. The new viruses include Iflaviruses, Rhabdoviruses, Nodaviruses, and Reoviruses, and members of unclassified lineages distantly related to Negeviruses, Sobemoviruses, Poleroviruses, Flaviviridae, and Tombusviridae. Among these are close relatives of Drosophila X virus and Flock House virus, which we find in association with wild Drosophila immigrans. These two viruses are widely used in experimental studies but have not been previously reported to naturally infect Drosophila. Although we detect no new DNA viruses, in D. immigrans and Drosophila obscura, we identify sequences very closely related to Armadillidium vulgare iridescent virus (Invertebrate iridescent virus 31), bringing the total number of DNA viruses found in the Drosophilidae to three. |
format | Online Article Text |
id | pubmed-4915790 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Libertas Academica |
record_format | MEDLINE/PubMed |
spelling | pubmed-49157902016-07-01 Twenty-Five New Viruses Associated with the Drosophilidae (Diptera) Webster, Claire L. Longdon, Ben Lewis, Samuel H. Obbard, Darren J. Evol Bioinform Online Original Research Drosophila melanogaster is an important laboratory model for studies of antiviral immunity in invertebrates, and Drosophila species provide a valuable system to study virus host range and host switching. Here, we use metagenomic RNA sequencing of about 1600 adult flies to discover 25 new RNA viruses associated with six different drosophilid hosts in the wild. We also provide a comprehensive listing of viruses previously reported from the Drosophilidae. The new viruses include Iflaviruses, Rhabdoviruses, Nodaviruses, and Reoviruses, and members of unclassified lineages distantly related to Negeviruses, Sobemoviruses, Poleroviruses, Flaviviridae, and Tombusviridae. Among these are close relatives of Drosophila X virus and Flock House virus, which we find in association with wild Drosophila immigrans. These two viruses are widely used in experimental studies but have not been previously reported to naturally infect Drosophila. Although we detect no new DNA viruses, in D. immigrans and Drosophila obscura, we identify sequences very closely related to Armadillidium vulgare iridescent virus (Invertebrate iridescent virus 31), bringing the total number of DNA viruses found in the Drosophilidae to three. Libertas Academica 2016-06-20 /pmc/articles/PMC4915790/ /pubmed/27375356 http://dx.doi.org/10.4137/EBO.S39454 Text en © 2016 the author(s), publisher and licensee Libertas Academica Ltd. This is an open-access article distributed under the terms of the Creative Commons CC-BY-NC 3.0 license. |
spellingShingle | Original Research Webster, Claire L. Longdon, Ben Lewis, Samuel H. Obbard, Darren J. Twenty-Five New Viruses Associated with the Drosophilidae (Diptera) |
title | Twenty-Five New Viruses Associated with the Drosophilidae (Diptera) |
title_full | Twenty-Five New Viruses Associated with the Drosophilidae (Diptera) |
title_fullStr | Twenty-Five New Viruses Associated with the Drosophilidae (Diptera) |
title_full_unstemmed | Twenty-Five New Viruses Associated with the Drosophilidae (Diptera) |
title_short | Twenty-Five New Viruses Associated with the Drosophilidae (Diptera) |
title_sort | twenty-five new viruses associated with the drosophilidae (diptera) |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4915790/ https://www.ncbi.nlm.nih.gov/pubmed/27375356 http://dx.doi.org/10.4137/EBO.S39454 |
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