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Completion of the Entire Hepatitis C Virus Life Cycle in Vero Cells Derived from Monkey Kidney

A hepatitis C virus (HCV) cell culture system incorporating the JFH-1 strain and the human hepatoma cell line HuH-7 enabled the production of infectious HCV particles. Several host factors were identified as essential for HCV replication. Supplementation of these factors in nonhepatic human cell lin...

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Autores principales: Murayama, Asako, Sugiyama, Nao, Wakita, Takaji, Kato, Takanobu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4916372/
https://www.ncbi.nlm.nih.gov/pubmed/27302754
http://dx.doi.org/10.1128/mBio.00273-16
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author Murayama, Asako
Sugiyama, Nao
Wakita, Takaji
Kato, Takanobu
author_facet Murayama, Asako
Sugiyama, Nao
Wakita, Takaji
Kato, Takanobu
author_sort Murayama, Asako
collection PubMed
description A hepatitis C virus (HCV) cell culture system incorporating the JFH-1 strain and the human hepatoma cell line HuH-7 enabled the production of infectious HCV particles. Several host factors were identified as essential for HCV replication. Supplementation of these factors in nonhepatic human cell lines enabled HCV replication and particle production. Vero cells established from monkey kidney are commonly used for the production of vaccines against a variety of viruses. In this study, we aimed to establish a novel Vero cell line to reconstruct the HCV life cycle. Unmodified Vero cells did not allow HCV infection or replication. The expression of microRNA 122 (miR-122), an essential factor for HCV replication, is notably low in Vero cells. Therefore, we supplemented Vero cells with miR-122 and found that HCV replication was enhanced. However, Vero cells that expressed miR-122 still did not allow HCV infection. We supplemented HCV receptor molecules and found that scavenger receptor class B type I (SRBI) was essential for HCV infection in Vero cells. The supplementation of apolipoprotein E (ApoE), a host factor important for virus production, enabled the production of infectious virus in Vero cells. Finally, we created a Vero cell line that expressed the essential factors miR-122, SRBI, and ApoE; the entire HCV life cycle, including infection, replication, and infectious virus production, was completed in these cells. In conclusion, we demonstrated that miR-122, SRBI, and ApoE were necessary and sufficient for the completion of the entire HCV life cycle in nonhuman, nonhepatic Vero cells.
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spelling pubmed-49163722016-06-23 Completion of the Entire Hepatitis C Virus Life Cycle in Vero Cells Derived from Monkey Kidney Murayama, Asako Sugiyama, Nao Wakita, Takaji Kato, Takanobu mBio Research Article A hepatitis C virus (HCV) cell culture system incorporating the JFH-1 strain and the human hepatoma cell line HuH-7 enabled the production of infectious HCV particles. Several host factors were identified as essential for HCV replication. Supplementation of these factors in nonhepatic human cell lines enabled HCV replication and particle production. Vero cells established from monkey kidney are commonly used for the production of vaccines against a variety of viruses. In this study, we aimed to establish a novel Vero cell line to reconstruct the HCV life cycle. Unmodified Vero cells did not allow HCV infection or replication. The expression of microRNA 122 (miR-122), an essential factor for HCV replication, is notably low in Vero cells. Therefore, we supplemented Vero cells with miR-122 and found that HCV replication was enhanced. However, Vero cells that expressed miR-122 still did not allow HCV infection. We supplemented HCV receptor molecules and found that scavenger receptor class B type I (SRBI) was essential for HCV infection in Vero cells. The supplementation of apolipoprotein E (ApoE), a host factor important for virus production, enabled the production of infectious virus in Vero cells. Finally, we created a Vero cell line that expressed the essential factors miR-122, SRBI, and ApoE; the entire HCV life cycle, including infection, replication, and infectious virus production, was completed in these cells. In conclusion, we demonstrated that miR-122, SRBI, and ApoE were necessary and sufficient for the completion of the entire HCV life cycle in nonhuman, nonhepatic Vero cells. American Society for Microbiology 2016-06-14 /pmc/articles/PMC4916372/ /pubmed/27302754 http://dx.doi.org/10.1128/mBio.00273-16 Text en Copyright © 2016 Murayama et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Murayama, Asako
Sugiyama, Nao
Wakita, Takaji
Kato, Takanobu
Completion of the Entire Hepatitis C Virus Life Cycle in Vero Cells Derived from Monkey Kidney
title Completion of the Entire Hepatitis C Virus Life Cycle in Vero Cells Derived from Monkey Kidney
title_full Completion of the Entire Hepatitis C Virus Life Cycle in Vero Cells Derived from Monkey Kidney
title_fullStr Completion of the Entire Hepatitis C Virus Life Cycle in Vero Cells Derived from Monkey Kidney
title_full_unstemmed Completion of the Entire Hepatitis C Virus Life Cycle in Vero Cells Derived from Monkey Kidney
title_short Completion of the Entire Hepatitis C Virus Life Cycle in Vero Cells Derived from Monkey Kidney
title_sort completion of the entire hepatitis c virus life cycle in vero cells derived from monkey kidney
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4916372/
https://www.ncbi.nlm.nih.gov/pubmed/27302754
http://dx.doi.org/10.1128/mBio.00273-16
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