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Identification of a Novel Enterovirus Species in Rhesus Macaque in China

Recent studies of Enterovirus (EV) in nonhuman primates (NHPs), which could act as a source of future emerging human viral diseases, have boosted interest in the search for novel EVs. Here, a highly divergent strain of EV, tentatively named SEV-gx, was identified by viral metagenomic analysis from s...

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Autores principales: Ao, Yuan-yun, Yu, Jie-mei, Zhang, Cui-yuan, Xin, Yun-yun, Li, Li-li, Duan, Zhao-jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4916455/
https://www.ncbi.nlm.nih.gov/pubmed/27329349
http://dx.doi.org/10.1038/srep28526
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author Ao, Yuan-yun
Yu, Jie-mei
Zhang, Cui-yuan
Xin, Yun-yun
Li, Li-li
Duan, Zhao-jun
author_facet Ao, Yuan-yun
Yu, Jie-mei
Zhang, Cui-yuan
Xin, Yun-yun
Li, Li-li
Duan, Zhao-jun
author_sort Ao, Yuan-yun
collection PubMed
description Recent studies of Enterovirus (EV) in nonhuman primates (NHPs), which could act as a source of future emerging human viral diseases, have boosted interest in the search for novel EVs. Here, a highly divergent strain of EV, tentatively named SEV-gx, was identified by viral metagenomic analysis from stool samples of rhesus macaques in China. In total, 27 of 280 (9.6%) faecal samples from rhesus macaques were positive for SEV-gx. Its complete genomic sequence is 7,367 nucleotide (nt). Genomic analyses showed that it has a standard genomic organisation for EVs, being more closely related to EV-J strains (approximately 54.0%, 43.0–44.1%, 52.3–55.2%, 61.1–62.7% and 64.0% amino acids identity in polyprotein, P1, P2 and P3 and combined 2C/3CD regions, respectively). It was also shown to have genome characteristics typical of EVs. Phylogenetic analysis of P1, 2C and 3CD aa indicated that SEV-gx can be classified as a distinct cluster in the EVs. All of this evidence demonstrates SEV-gx is a novel species (tentatively named EV-K) in the EV genus, which contributes to our understanding of the genetic diversity and evolution of EVs. Further studies are needed to investigate the potential pathogenicity of SEV-gx in NHPs and humans.
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spelling pubmed-49164552016-06-27 Identification of a Novel Enterovirus Species in Rhesus Macaque in China Ao, Yuan-yun Yu, Jie-mei Zhang, Cui-yuan Xin, Yun-yun Li, Li-li Duan, Zhao-jun Sci Rep Article Recent studies of Enterovirus (EV) in nonhuman primates (NHPs), which could act as a source of future emerging human viral diseases, have boosted interest in the search for novel EVs. Here, a highly divergent strain of EV, tentatively named SEV-gx, was identified by viral metagenomic analysis from stool samples of rhesus macaques in China. In total, 27 of 280 (9.6%) faecal samples from rhesus macaques were positive for SEV-gx. Its complete genomic sequence is 7,367 nucleotide (nt). Genomic analyses showed that it has a standard genomic organisation for EVs, being more closely related to EV-J strains (approximately 54.0%, 43.0–44.1%, 52.3–55.2%, 61.1–62.7% and 64.0% amino acids identity in polyprotein, P1, P2 and P3 and combined 2C/3CD regions, respectively). It was also shown to have genome characteristics typical of EVs. Phylogenetic analysis of P1, 2C and 3CD aa indicated that SEV-gx can be classified as a distinct cluster in the EVs. All of this evidence demonstrates SEV-gx is a novel species (tentatively named EV-K) in the EV genus, which contributes to our understanding of the genetic diversity and evolution of EVs. Further studies are needed to investigate the potential pathogenicity of SEV-gx in NHPs and humans. Nature Publishing Group 2016-06-22 /pmc/articles/PMC4916455/ /pubmed/27329349 http://dx.doi.org/10.1038/srep28526 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ao, Yuan-yun
Yu, Jie-mei
Zhang, Cui-yuan
Xin, Yun-yun
Li, Li-li
Duan, Zhao-jun
Identification of a Novel Enterovirus Species in Rhesus Macaque in China
title Identification of a Novel Enterovirus Species in Rhesus Macaque in China
title_full Identification of a Novel Enterovirus Species in Rhesus Macaque in China
title_fullStr Identification of a Novel Enterovirus Species in Rhesus Macaque in China
title_full_unstemmed Identification of a Novel Enterovirus Species in Rhesus Macaque in China
title_short Identification of a Novel Enterovirus Species in Rhesus Macaque in China
title_sort identification of a novel enterovirus species in rhesus macaque in china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4916455/
https://www.ncbi.nlm.nih.gov/pubmed/27329349
http://dx.doi.org/10.1038/srep28526
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