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Foetal bovine serum-derived exosomes affect yield and phenotype of human cardiac progenitor cell culture

[Image: see text] Introduction: Cardiac progenitor cells (CPCs) represent a powerful tool in cardiac regenerative medicine. Pre-clinical studies suggest that most of the beneficial effects promoted by the injected cells are due to their paracrine activity exerted on endogenous cells and tissue. Exos...

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Autores principales: Angelini, Francesco, Ionta, Vittoria, Rossi, Fabrizio, Miraldi, Fabio, Messina, Elisa, Giacomello, Alessandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4916547/
https://www.ncbi.nlm.nih.gov/pubmed/27340620
http://dx.doi.org/10.15171/bi.2016.03
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author Angelini, Francesco
Ionta, Vittoria
Rossi, Fabrizio
Miraldi, Fabio
Messina, Elisa
Giacomello, Alessandro
author_facet Angelini, Francesco
Ionta, Vittoria
Rossi, Fabrizio
Miraldi, Fabio
Messina, Elisa
Giacomello, Alessandro
author_sort Angelini, Francesco
collection PubMed
description [Image: see text] Introduction: Cardiac progenitor cells (CPCs) represent a powerful tool in cardiac regenerative medicine. Pre-clinical studies suggest that most of the beneficial effects promoted by the injected cells are due to their paracrine activity exerted on endogenous cells and tissue. Exosomes are candidate mediators of this paracrine effects. According to their potential, many researchers have focused on characterizing exosomes derived from specific cell types, but, up until now, only few studies have analyzed the possible in vitro effects of bovine serum-derived exosomes on cell proliferation or differentiation. Methods: The aim of this study was to analyse, from a qualitative and quantitative point of view, the in vitro effects of bovine serum exosomes on human CPCs cultured either as cardiospheres or as monolayers of cardiosphere-forming cells. Results: Effects on proliferation, yield and molecular patterning were detected. We show, for the first time, that exogenous bovine exosomes support the proliferation and migration of human cardiosphere-forming cells, and that their depletion affects cardiospheres formation, in terms of size, yield and extra-cellular matrix production. Conclusion: These results stress the importance of considering differential biological effects of exogenous cell culture supplements on the final phenotype of primary human cell cultures.
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spelling pubmed-49165472016-06-23 Foetal bovine serum-derived exosomes affect yield and phenotype of human cardiac progenitor cell culture Angelini, Francesco Ionta, Vittoria Rossi, Fabrizio Miraldi, Fabio Messina, Elisa Giacomello, Alessandro Bioimpacts Original Article [Image: see text] Introduction: Cardiac progenitor cells (CPCs) represent a powerful tool in cardiac regenerative medicine. Pre-clinical studies suggest that most of the beneficial effects promoted by the injected cells are due to their paracrine activity exerted on endogenous cells and tissue. Exosomes are candidate mediators of this paracrine effects. According to their potential, many researchers have focused on characterizing exosomes derived from specific cell types, but, up until now, only few studies have analyzed the possible in vitro effects of bovine serum-derived exosomes on cell proliferation or differentiation. Methods: The aim of this study was to analyse, from a qualitative and quantitative point of view, the in vitro effects of bovine serum exosomes on human CPCs cultured either as cardiospheres or as monolayers of cardiosphere-forming cells. Results: Effects on proliferation, yield and molecular patterning were detected. We show, for the first time, that exogenous bovine exosomes support the proliferation and migration of human cardiosphere-forming cells, and that their depletion affects cardiospheres formation, in terms of size, yield and extra-cellular matrix production. Conclusion: These results stress the importance of considering differential biological effects of exogenous cell culture supplements on the final phenotype of primary human cell cultures. Tabriz University of Medical Sciences 2016 2016-03-28 /pmc/articles/PMC4916547/ /pubmed/27340620 http://dx.doi.org/10.15171/bi.2016.03 Text en © 2016 The Author(s) This work is published by BioImpacts as an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Article
Angelini, Francesco
Ionta, Vittoria
Rossi, Fabrizio
Miraldi, Fabio
Messina, Elisa
Giacomello, Alessandro
Foetal bovine serum-derived exosomes affect yield and phenotype of human cardiac progenitor cell culture
title Foetal bovine serum-derived exosomes affect yield and phenotype of human cardiac progenitor cell culture
title_full Foetal bovine serum-derived exosomes affect yield and phenotype of human cardiac progenitor cell culture
title_fullStr Foetal bovine serum-derived exosomes affect yield and phenotype of human cardiac progenitor cell culture
title_full_unstemmed Foetal bovine serum-derived exosomes affect yield and phenotype of human cardiac progenitor cell culture
title_short Foetal bovine serum-derived exosomes affect yield and phenotype of human cardiac progenitor cell culture
title_sort foetal bovine serum-derived exosomes affect yield and phenotype of human cardiac progenitor cell culture
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4916547/
https://www.ncbi.nlm.nih.gov/pubmed/27340620
http://dx.doi.org/10.15171/bi.2016.03
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