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Differentiation of Rat Bone Marrow Mesenchymal Stem Cells Into Neuron-Like Cells In Vitro and Co-Cultured with Biological Scaffold as Transplantation Carrier

BACKGROUND: Autograft and allograft transplantation are used to prompt the regeneration of axons after nerve injury. However, the poor self-regeneration caused by the glial scar and growth inhibitory factors after neuronal necrosis limit the efficacy of these methods. The purpose of this study was t...

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Autores principales: Yue, Wei, Yan, Feng, Zhang, Yue-Lin, Liu, Shu-Ling, Hou, Shu-Ping, Mao, Guo-Chao, Liu, Ning, Ji, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4917310/
https://www.ncbi.nlm.nih.gov/pubmed/27225035
http://dx.doi.org/10.12659/MSM.898441
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author Yue, Wei
Yan, Feng
Zhang, Yue-Lin
Liu, Shu-Ling
Hou, Shu-Ping
Mao, Guo-Chao
Liu, Ning
Ji, Yong
author_facet Yue, Wei
Yan, Feng
Zhang, Yue-Lin
Liu, Shu-Ling
Hou, Shu-Ping
Mao, Guo-Chao
Liu, Ning
Ji, Yong
author_sort Yue, Wei
collection PubMed
description BACKGROUND: Autograft and allograft transplantation are used to prompt the regeneration of axons after nerve injury. However, the poor self-regeneration caused by the glial scar and growth inhibitory factors after neuronal necrosis limit the efficacy of these methods. The purpose of this study was to develop a new chitosan porous scaffold for cell seeding. MATERIAL/METHODS: The bone marrow mesenchymal stem cells (BMSCs) and tissue-engineered biomaterial scaffold compound were constructed and co-cultured in vitro with the differentiated BMSCs of Wistar rats and chitosan scaffold in a 3D environment. The purity of the third-generation BMSCs culture was identified using flow cytometry and assessment of induced neuronal differentiation. The scaffolds were prepared by the freeze-drying method. The internal structure of scaffolds and the change of cells’ growth and morphology were observed under a scanning electron microscope. The proliferation of cells was detected with the MTT method. RESULTS: On day 5 there was a significant difference in the absorbance value of the experimental group (0.549±0.0256) and the control group (0.487±0.0357) (P>0.05); but on day 7 there was no significant difference in the proliferation of the experimental group (0.751±0.011) and the control group and (0.78±0.017) (P>0.05). CONCLUSIONS: Tissue engineering technology can provide a carrier for cells seeding and is expected to become an effective method for the regeneration and repair of nerve cells. Our study showed that chitosan porous scaffolds can be used for such purposes.
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spelling pubmed-49173102016-06-30 Differentiation of Rat Bone Marrow Mesenchymal Stem Cells Into Neuron-Like Cells In Vitro and Co-Cultured with Biological Scaffold as Transplantation Carrier Yue, Wei Yan, Feng Zhang, Yue-Lin Liu, Shu-Ling Hou, Shu-Ping Mao, Guo-Chao Liu, Ning Ji, Yong Med Sci Monit Lab/In Vitro Research BACKGROUND: Autograft and allograft transplantation are used to prompt the regeneration of axons after nerve injury. However, the poor self-regeneration caused by the glial scar and growth inhibitory factors after neuronal necrosis limit the efficacy of these methods. The purpose of this study was to develop a new chitosan porous scaffold for cell seeding. MATERIAL/METHODS: The bone marrow mesenchymal stem cells (BMSCs) and tissue-engineered biomaterial scaffold compound were constructed and co-cultured in vitro with the differentiated BMSCs of Wistar rats and chitosan scaffold in a 3D environment. The purity of the third-generation BMSCs culture was identified using flow cytometry and assessment of induced neuronal differentiation. The scaffolds were prepared by the freeze-drying method. The internal structure of scaffolds and the change of cells’ growth and morphology were observed under a scanning electron microscope. The proliferation of cells was detected with the MTT method. RESULTS: On day 5 there was a significant difference in the absorbance value of the experimental group (0.549±0.0256) and the control group (0.487±0.0357) (P>0.05); but on day 7 there was no significant difference in the proliferation of the experimental group (0.751±0.011) and the control group and (0.78±0.017) (P>0.05). CONCLUSIONS: Tissue engineering technology can provide a carrier for cells seeding and is expected to become an effective method for the regeneration and repair of nerve cells. Our study showed that chitosan porous scaffolds can be used for such purposes. International Scientific Literature, Inc. 2016-05-26 /pmc/articles/PMC4917310/ /pubmed/27225035 http://dx.doi.org/10.12659/MSM.898441 Text en © Med Sci Monit, 2016 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
spellingShingle Lab/In Vitro Research
Yue, Wei
Yan, Feng
Zhang, Yue-Lin
Liu, Shu-Ling
Hou, Shu-Ping
Mao, Guo-Chao
Liu, Ning
Ji, Yong
Differentiation of Rat Bone Marrow Mesenchymal Stem Cells Into Neuron-Like Cells In Vitro and Co-Cultured with Biological Scaffold as Transplantation Carrier
title Differentiation of Rat Bone Marrow Mesenchymal Stem Cells Into Neuron-Like Cells In Vitro and Co-Cultured with Biological Scaffold as Transplantation Carrier
title_full Differentiation of Rat Bone Marrow Mesenchymal Stem Cells Into Neuron-Like Cells In Vitro and Co-Cultured with Biological Scaffold as Transplantation Carrier
title_fullStr Differentiation of Rat Bone Marrow Mesenchymal Stem Cells Into Neuron-Like Cells In Vitro and Co-Cultured with Biological Scaffold as Transplantation Carrier
title_full_unstemmed Differentiation of Rat Bone Marrow Mesenchymal Stem Cells Into Neuron-Like Cells In Vitro and Co-Cultured with Biological Scaffold as Transplantation Carrier
title_short Differentiation of Rat Bone Marrow Mesenchymal Stem Cells Into Neuron-Like Cells In Vitro and Co-Cultured with Biological Scaffold as Transplantation Carrier
title_sort differentiation of rat bone marrow mesenchymal stem cells into neuron-like cells in vitro and co-cultured with biological scaffold as transplantation carrier
topic Lab/In Vitro Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4917310/
https://www.ncbi.nlm.nih.gov/pubmed/27225035
http://dx.doi.org/10.12659/MSM.898441
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