Identification of Raoultella terrigena as a Rare Causative Agent of Subungual Abscess Based on 16S rRNA and Housekeeping Gene Sequencing

A 63-year-old-man was admitted to our hospital with severe subungual abscess. Bacteria were isolated from pus samples, and an inconsistent identification was shown by VITEK 2 system and MALDI-TOF mass spectrometry as Raoultella planticola and Raoultella terrigena, respectively. Molecular identificat...

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Autores principales: Wang, Yu, Jiang, Xiawei, Xu, Zemin, Ying, Chaoqun, Yu, Wei, Xiao, Yonghong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Case Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4917704/
https://www.ncbi.nlm.nih.gov/pubmed/27379169
http://dx.doi.org/10.1155/2016/3879635
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author Wang, Yu
Jiang, Xiawei
Xu, Zemin
Ying, Chaoqun
Yu, Wei
Xiao, Yonghong
author_facet Wang, Yu
Jiang, Xiawei
Xu, Zemin
Ying, Chaoqun
Yu, Wei
Xiao, Yonghong
author_sort Wang, Yu
collection PubMed
description A 63-year-old-man was admitted to our hospital with severe subungual abscess. Bacteria were isolated from pus samples, and an inconsistent identification was shown by VITEK 2 system and MALDI-TOF mass spectrometry as Raoultella planticola and Raoultella terrigena, respectively. Molecular identification by 16S rRNA sequencing suggested that the isolate is R. terrigena, and this was further demonstrated by sequencing three housekeeping genes (rpoB, gyrA, and parC) with phylogenetic analysis. To our knowledge, this is the first report of subungual abscess caused by R. terrigena, a rare case of human infection due to soil bacterium. Our study highlights the technique importance on this pathogen identification.
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spelling pubmed-49177042016-07-04 Identification of Raoultella terrigena as a Rare Causative Agent of Subungual Abscess Based on 16S rRNA and Housekeeping Gene Sequencing Wang, Yu Jiang, Xiawei Xu, Zemin Ying, Chaoqun Yu, Wei Xiao, Yonghong Can J Infect Dis Med Microbiol Case Report A 63-year-old-man was admitted to our hospital with severe subungual abscess. Bacteria were isolated from pus samples, and an inconsistent identification was shown by VITEK 2 system and MALDI-TOF mass spectrometry as Raoultella planticola and Raoultella terrigena, respectively. Molecular identification by 16S rRNA sequencing suggested that the isolate is R. terrigena, and this was further demonstrated by sequencing three housekeeping genes (rpoB, gyrA, and parC) with phylogenetic analysis. To our knowledge, this is the first report of subungual abscess caused by R. terrigena, a rare case of human infection due to soil bacterium. Our study highlights the technique importance on this pathogen identification. Hindawi Publishing Corporation 2016 2016-06-09 /pmc/articles/PMC4917704/ /pubmed/27379169 http://dx.doi.org/10.1155/2016/3879635 Text en Copyright © 2016 Yu Wang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Case Report
Wang, Yu
Jiang, Xiawei
Xu, Zemin
Ying, Chaoqun
Yu, Wei
Xiao, Yonghong
Identification of Raoultella terrigena as a Rare Causative Agent of Subungual Abscess Based on 16S rRNA and Housekeeping Gene Sequencing
title Identification of Raoultella terrigena as a Rare Causative Agent of Subungual Abscess Based on 16S rRNA and Housekeeping Gene Sequencing
title_full Identification of Raoultella terrigena as a Rare Causative Agent of Subungual Abscess Based on 16S rRNA and Housekeeping Gene Sequencing
title_fullStr Identification of Raoultella terrigena as a Rare Causative Agent of Subungual Abscess Based on 16S rRNA and Housekeeping Gene Sequencing
title_full_unstemmed Identification of Raoultella terrigena as a Rare Causative Agent of Subungual Abscess Based on 16S rRNA and Housekeeping Gene Sequencing
title_short Identification of Raoultella terrigena as a Rare Causative Agent of Subungual Abscess Based on 16S rRNA and Housekeeping Gene Sequencing
title_sort identification of raoultella terrigena as a rare causative agent of subungual abscess based on 16s rrna and housekeeping gene sequencing
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4917704/
https://www.ncbi.nlm.nih.gov/pubmed/27379169
http://dx.doi.org/10.1155/2016/3879635
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