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Designing, construction and characterization of genetically encoded FRET-based nanosensor for real time monitoring of lysine flux in living cells
BACKGROUND: Engineering microorganisms in order to improve the metabolite flux needs a detailed knowledge of the concentrations and flux rates of metabolites and metabolic intermediates in vivo. Fluorescence resonance energy transfer (FRET) based genetically encoded nanosensors represent a promising...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4917951/ https://www.ncbi.nlm.nih.gov/pubmed/27334743 http://dx.doi.org/10.1186/s12951-016-0204-y |