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Endothelial progenitor cells are differentially impaired in ANCA-associated vasculitis compared to healthy controls
BACKGROUND: Endothelial progenitor cells (EPC) are of major importance in vascular repair under healthy circumstances. Vascular injury in need of repair occurs frequently in ANCA-associated vasculitis (AAV). A specialized T cell subset enhancing EPC function and differentiation has recently been des...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918016/ https://www.ncbi.nlm.nih.gov/pubmed/27338585 http://dx.doi.org/10.1186/s13075-016-1044-8 |
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author | Wilde, B. Mertens, A. Arends, S. J. Rouhl, R. P. Bijleveld, R. Huitema, J. Timmermans, S. A. Damoiseaux, J. Witzke, O. Duijvestijn, A. M. van Paassen, P. van Oostenbrugge, R. J. Cohen Tervaert, J. W. |
author_facet | Wilde, B. Mertens, A. Arends, S. J. Rouhl, R. P. Bijleveld, R. Huitema, J. Timmermans, S. A. Damoiseaux, J. Witzke, O. Duijvestijn, A. M. van Paassen, P. van Oostenbrugge, R. J. Cohen Tervaert, J. W. |
author_sort | Wilde, B. |
collection | PubMed |
description | BACKGROUND: Endothelial progenitor cells (EPC) are of major importance in vascular repair under healthy circumstances. Vascular injury in need of repair occurs frequently in ANCA-associated vasculitis (AAV). A specialized T cell subset enhancing EPC function and differentiation has recently been described. These angiogenic T cells (T(ang)) may have an important impact on the vascular repair process. Therefore, the aim of our study was to investigate EPC and T(ang) in AAV. METHODS: Fifty-three patients suffering from AAV and 29 healthy controls (HC) were enrolled in our study. Forty-four patients were in remission, nine patients were in active state of disease. Patients were either untreated or were under monotherapy with low-dose steroids (max. 5 mg/day) at the time of sampling. Circulating EPC and T(ang) were determined by flow cytometry (FACS). The functional capacity of EPC was assessed by established cell culture methods. RESULTS: Circulating EPC were significantly decreased in AAV as compared to HC. The capacity of EPC to differentiate and proliferate was differentially impaired in patients as compared to HC. The outgrowth of endothelial colony-forming cells (ECFC) was severely decreased in patients whereas colony-forming units-endothelial cell (CFU-EC) outgrowth was unaffected. ECFC and CFU-EC differentiation was strictly T cell-dependent. Patients with a relapsing disease course had an impaired ECFC outgrowth and expansion of T(ang) as compared to patients with a stable, nonrelapsing disease. CONCLUSIONS: The differentiation process of EPC is impaired in AAV. This may favor insufficient vascular repair promoting a relapsing disease course. Finally, these factors may explain a higher cardiovascular morbidity as has been previously documented in AAV. |
format | Online Article Text |
id | pubmed-4918016 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-49180162016-06-24 Endothelial progenitor cells are differentially impaired in ANCA-associated vasculitis compared to healthy controls Wilde, B. Mertens, A. Arends, S. J. Rouhl, R. P. Bijleveld, R. Huitema, J. Timmermans, S. A. Damoiseaux, J. Witzke, O. Duijvestijn, A. M. van Paassen, P. van Oostenbrugge, R. J. Cohen Tervaert, J. W. Arthritis Res Ther Research Article BACKGROUND: Endothelial progenitor cells (EPC) are of major importance in vascular repair under healthy circumstances. Vascular injury in need of repair occurs frequently in ANCA-associated vasculitis (AAV). A specialized T cell subset enhancing EPC function and differentiation has recently been described. These angiogenic T cells (T(ang)) may have an important impact on the vascular repair process. Therefore, the aim of our study was to investigate EPC and T(ang) in AAV. METHODS: Fifty-three patients suffering from AAV and 29 healthy controls (HC) were enrolled in our study. Forty-four patients were in remission, nine patients were in active state of disease. Patients were either untreated or were under monotherapy with low-dose steroids (max. 5 mg/day) at the time of sampling. Circulating EPC and T(ang) were determined by flow cytometry (FACS). The functional capacity of EPC was assessed by established cell culture methods. RESULTS: Circulating EPC were significantly decreased in AAV as compared to HC. The capacity of EPC to differentiate and proliferate was differentially impaired in patients as compared to HC. The outgrowth of endothelial colony-forming cells (ECFC) was severely decreased in patients whereas colony-forming units-endothelial cell (CFU-EC) outgrowth was unaffected. ECFC and CFU-EC differentiation was strictly T cell-dependent. Patients with a relapsing disease course had an impaired ECFC outgrowth and expansion of T(ang) as compared to patients with a stable, nonrelapsing disease. CONCLUSIONS: The differentiation process of EPC is impaired in AAV. This may favor insufficient vascular repair promoting a relapsing disease course. Finally, these factors may explain a higher cardiovascular morbidity as has been previously documented in AAV. BioMed Central 2016-06-23 2016 /pmc/articles/PMC4918016/ /pubmed/27338585 http://dx.doi.org/10.1186/s13075-016-1044-8 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Wilde, B. Mertens, A. Arends, S. J. Rouhl, R. P. Bijleveld, R. Huitema, J. Timmermans, S. A. Damoiseaux, J. Witzke, O. Duijvestijn, A. M. van Paassen, P. van Oostenbrugge, R. J. Cohen Tervaert, J. W. Endothelial progenitor cells are differentially impaired in ANCA-associated vasculitis compared to healthy controls |
title | Endothelial progenitor cells are differentially impaired in ANCA-associated vasculitis compared to healthy controls |
title_full | Endothelial progenitor cells are differentially impaired in ANCA-associated vasculitis compared to healthy controls |
title_fullStr | Endothelial progenitor cells are differentially impaired in ANCA-associated vasculitis compared to healthy controls |
title_full_unstemmed | Endothelial progenitor cells are differentially impaired in ANCA-associated vasculitis compared to healthy controls |
title_short | Endothelial progenitor cells are differentially impaired in ANCA-associated vasculitis compared to healthy controls |
title_sort | endothelial progenitor cells are differentially impaired in anca-associated vasculitis compared to healthy controls |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918016/ https://www.ncbi.nlm.nih.gov/pubmed/27338585 http://dx.doi.org/10.1186/s13075-016-1044-8 |
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