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Danshen enhanced the estrogenic effects of Qing E formula in ovariectomized rats

BACKGROUND: Menopause is characterized by a decrease in life quality due to the appearance of uncomfortable symptoms. Nowadays, Understanding menopause-associated pathophysiology and developing new strategies to improve the treatment of menopausal-associated symptoms is an important issue. Our study...

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Autores principales: Zhang, Jian-mei, Li, Jin, Liu, Er-wei, Wang, Hong, Fan, Guan-wei, Wang, Yue-fei, Zhu, Yan, Ma, Shang-wei, Gao, Xiu-mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918020/
https://www.ncbi.nlm.nih.gov/pubmed/27339619
http://dx.doi.org/10.1186/s12906-016-1146-5
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author Zhang, Jian-mei
Li, Jin
Liu, Er-wei
Wang, Hong
Fan, Guan-wei
Wang, Yue-fei
Zhu, Yan
Ma, Shang-wei
Gao, Xiu-mei
author_facet Zhang, Jian-mei
Li, Jin
Liu, Er-wei
Wang, Hong
Fan, Guan-wei
Wang, Yue-fei
Zhu, Yan
Ma, Shang-wei
Gao, Xiu-mei
author_sort Zhang, Jian-mei
collection PubMed
description BACKGROUND: Menopause is characterized by a decrease in life quality due to the appearance of uncomfortable symptoms. Nowadays, Understanding menopause-associated pathophysiology and developing new strategies to improve the treatment of menopausal-associated symptoms is an important issue. Our study was to evaluate the synergistic effects of Danshen (salvia miltiorrhiza bunge) and the phytoestrogenic effects of 3 modified Qing E formulas, to explore a better formula for menopausal disorders. METHODS: 100 rats were randomized into 5 groups: Sham (Sham operation group), OVX (model group of ovariectomized rat), BDL (group with low concentration of Qing E Formula), BDH (group with high concentration of Qing E Formula) and BDD (group with high concentration of Qing E Formula Plus Danshen), receiving vehicle and extract of different modified Qing E formula respectively. The food intake, body weight, uterus weight, blood levels of triglycerides (TG), total cholesterol (TC) and cholesterol fractions were assessed. The mammary glands and uterus were morphologically analyzed. The bone density of tibias were measured by peripheral quantitative computed tomography (pQCT). Additionally, luciferase induction assays were performed in Hela cells with the mixtures derived from Qing E formula plus Danshen (BDD). RESULTS: Qing E formula plus Danshen significantly increased the uterus wet weight, enhanced the thickness of uterine wall, endometrial epithelium and glandular epithelium, improved trabecular bone and total density evidently, reduced the levels of low density lipoprotein cholesterol (LDL-C) and TG, possessed notable estrogen receptor beta (ERβ) and estrogen receptor alpha (ERα) agonist activity. CONCLUSION: Qing E formula plus Danshen exerted more evident estrogen-like effects, thus it has a potential therapeutic use to treat menopausal disorders.
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spelling pubmed-49180202016-06-24 Danshen enhanced the estrogenic effects of Qing E formula in ovariectomized rats Zhang, Jian-mei Li, Jin Liu, Er-wei Wang, Hong Fan, Guan-wei Wang, Yue-fei Zhu, Yan Ma, Shang-wei Gao, Xiu-mei BMC Complement Altern Med Research Article BACKGROUND: Menopause is characterized by a decrease in life quality due to the appearance of uncomfortable symptoms. Nowadays, Understanding menopause-associated pathophysiology and developing new strategies to improve the treatment of menopausal-associated symptoms is an important issue. Our study was to evaluate the synergistic effects of Danshen (salvia miltiorrhiza bunge) and the phytoestrogenic effects of 3 modified Qing E formulas, to explore a better formula for menopausal disorders. METHODS: 100 rats were randomized into 5 groups: Sham (Sham operation group), OVX (model group of ovariectomized rat), BDL (group with low concentration of Qing E Formula), BDH (group with high concentration of Qing E Formula) and BDD (group with high concentration of Qing E Formula Plus Danshen), receiving vehicle and extract of different modified Qing E formula respectively. The food intake, body weight, uterus weight, blood levels of triglycerides (TG), total cholesterol (TC) and cholesterol fractions were assessed. The mammary glands and uterus were morphologically analyzed. The bone density of tibias were measured by peripheral quantitative computed tomography (pQCT). Additionally, luciferase induction assays were performed in Hela cells with the mixtures derived from Qing E formula plus Danshen (BDD). RESULTS: Qing E formula plus Danshen significantly increased the uterus wet weight, enhanced the thickness of uterine wall, endometrial epithelium and glandular epithelium, improved trabecular bone and total density evidently, reduced the levels of low density lipoprotein cholesterol (LDL-C) and TG, possessed notable estrogen receptor beta (ERβ) and estrogen receptor alpha (ERα) agonist activity. CONCLUSION: Qing E formula plus Danshen exerted more evident estrogen-like effects, thus it has a potential therapeutic use to treat menopausal disorders. BioMed Central 2016-06-23 /pmc/articles/PMC4918020/ /pubmed/27339619 http://dx.doi.org/10.1186/s12906-016-1146-5 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhang, Jian-mei
Li, Jin
Liu, Er-wei
Wang, Hong
Fan, Guan-wei
Wang, Yue-fei
Zhu, Yan
Ma, Shang-wei
Gao, Xiu-mei
Danshen enhanced the estrogenic effects of Qing E formula in ovariectomized rats
title Danshen enhanced the estrogenic effects of Qing E formula in ovariectomized rats
title_full Danshen enhanced the estrogenic effects of Qing E formula in ovariectomized rats
title_fullStr Danshen enhanced the estrogenic effects of Qing E formula in ovariectomized rats
title_full_unstemmed Danshen enhanced the estrogenic effects of Qing E formula in ovariectomized rats
title_short Danshen enhanced the estrogenic effects of Qing E formula in ovariectomized rats
title_sort danshen enhanced the estrogenic effects of qing e formula in ovariectomized rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918020/
https://www.ncbi.nlm.nih.gov/pubmed/27339619
http://dx.doi.org/10.1186/s12906-016-1146-5
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