Chlorella virus pyrimidine dimer glycosylase and Escherichia coli endonucleases IV and V have incision activity on 2,2,4-triamino-5(2H)-oxazolone

INTRODUCTION: 2,2,4-Triamino-5(2H)-oxazolone (Oz) in a DNA strand is an oxidation product of guanine and 8-oxo-7, 8-dihydroguanine, and such a lesion can cause G-to-C transversions. Previously, Fpg/Nei and Nth were shown to have incision activity on Oz. FINDINGS: We investigated the activities of ch...

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Autores principales: Kino, Katsuhito, Suzuki, Masayo, Morikawa, Masayuki, Kobayashi, Takanobu, Iwai, Shigenori, Miyazawa, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918129/
https://www.ncbi.nlm.nih.gov/pubmed/27350817
http://dx.doi.org/10.1186/s41021-015-0022-8
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author Kino, Katsuhito
Suzuki, Masayo
Morikawa, Masayuki
Kobayashi, Takanobu
Iwai, Shigenori
Miyazawa, Hiroshi
author_facet Kino, Katsuhito
Suzuki, Masayo
Morikawa, Masayuki
Kobayashi, Takanobu
Iwai, Shigenori
Miyazawa, Hiroshi
author_sort Kino, Katsuhito
collection PubMed
description INTRODUCTION: 2,2,4-Triamino-5(2H)-oxazolone (Oz) in a DNA strand is an oxidation product of guanine and 8-oxo-7, 8-dihydroguanine, and such a lesion can cause G-to-C transversions. Previously, Fpg/Nei and Nth were shown to have incision activity on Oz. FINDINGS: We investigated the activities of chlorella virus pyrimidine dimer glycosylase (cvPDG) and Escherichia coli endonucleases IV (Nfo) and V (Nfi) on Oz. Although the three enzymes have different repair mechanisms from Fpg/Nei and Nth, they still had incision activity on Oz. CONCLUSIONS: Given the incision activities of cvPDG, Nfo and Nfi on Oz in addition to Fpg/Nei and Nth, Oz is DNA damage that can be repaired by diverse enzymes.
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spelling pubmed-49181292016-06-27 Chlorella virus pyrimidine dimer glycosylase and Escherichia coli endonucleases IV and V have incision activity on 2,2,4-triamino-5(2H)-oxazolone Kino, Katsuhito Suzuki, Masayo Morikawa, Masayuki Kobayashi, Takanobu Iwai, Shigenori Miyazawa, Hiroshi Genes Environ Short Report INTRODUCTION: 2,2,4-Triamino-5(2H)-oxazolone (Oz) in a DNA strand is an oxidation product of guanine and 8-oxo-7, 8-dihydroguanine, and such a lesion can cause G-to-C transversions. Previously, Fpg/Nei and Nth were shown to have incision activity on Oz. FINDINGS: We investigated the activities of chlorella virus pyrimidine dimer glycosylase (cvPDG) and Escherichia coli endonucleases IV (Nfo) and V (Nfi) on Oz. Although the three enzymes have different repair mechanisms from Fpg/Nei and Nth, they still had incision activity on Oz. CONCLUSIONS: Given the incision activities of cvPDG, Nfo and Nfi on Oz in addition to Fpg/Nei and Nth, Oz is DNA damage that can be repaired by diverse enzymes. BioMed Central 2015-11-01 /pmc/articles/PMC4918129/ /pubmed/27350817 http://dx.doi.org/10.1186/s41021-015-0022-8 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Kino, Katsuhito
Suzuki, Masayo
Morikawa, Masayuki
Kobayashi, Takanobu
Iwai, Shigenori
Miyazawa, Hiroshi
Chlorella virus pyrimidine dimer glycosylase and Escherichia coli endonucleases IV and V have incision activity on 2,2,4-triamino-5(2H)-oxazolone
title Chlorella virus pyrimidine dimer glycosylase and Escherichia coli endonucleases IV and V have incision activity on 2,2,4-triamino-5(2H)-oxazolone
title_full Chlorella virus pyrimidine dimer glycosylase and Escherichia coli endonucleases IV and V have incision activity on 2,2,4-triamino-5(2H)-oxazolone
title_fullStr Chlorella virus pyrimidine dimer glycosylase and Escherichia coli endonucleases IV and V have incision activity on 2,2,4-triamino-5(2H)-oxazolone
title_full_unstemmed Chlorella virus pyrimidine dimer glycosylase and Escherichia coli endonucleases IV and V have incision activity on 2,2,4-triamino-5(2H)-oxazolone
title_short Chlorella virus pyrimidine dimer glycosylase and Escherichia coli endonucleases IV and V have incision activity on 2,2,4-triamino-5(2H)-oxazolone
title_sort chlorella virus pyrimidine dimer glycosylase and escherichia coli endonucleases iv and v have incision activity on 2,2,4-triamino-5(2h)-oxazolone
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918129/
https://www.ncbi.nlm.nih.gov/pubmed/27350817
http://dx.doi.org/10.1186/s41021-015-0022-8
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