Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses

Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses.

Detalles Bibliográficos
Autores principales: Waggoner, Jesse J., Gresh, Lionel, Mohamed-Hadley, Alisha, Ballesteros, Gabriela, Davila, Maria Jose Vargas, Tellez, Yolanda, Sahoo, Malaya K., Balmaseda, Angel, Harris, Eva, Pinsky, Benjamin A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2016
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918162/
https://www.ncbi.nlm.nih.gov/pubmed/27184629
http://dx.doi.org/10.3201/eid2207.160326
Descripción
Sumario:Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses.

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