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Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses

Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses.

Detalles Bibliográficos
Autores principales: Waggoner, Jesse J., Gresh, Lionel, Mohamed-Hadley, Alisha, Ballesteros, Gabriela, Davila, Maria Jose Vargas, Tellez, Yolanda, Sahoo, Malaya K., Balmaseda, Angel, Harris, Eva, Pinsky, Benjamin A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918162/
https://www.ncbi.nlm.nih.gov/pubmed/27184629
http://dx.doi.org/10.3201/eid2207.160326
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author Waggoner, Jesse J.
Gresh, Lionel
Mohamed-Hadley, Alisha
Ballesteros, Gabriela
Davila, Maria Jose Vargas
Tellez, Yolanda
Sahoo, Malaya K.
Balmaseda, Angel
Harris, Eva
Pinsky, Benjamin A.
author_facet Waggoner, Jesse J.
Gresh, Lionel
Mohamed-Hadley, Alisha
Ballesteros, Gabriela
Davila, Maria Jose Vargas
Tellez, Yolanda
Sahoo, Malaya K.
Balmaseda, Angel
Harris, Eva
Pinsky, Benjamin A.
author_sort Waggoner, Jesse J.
collection PubMed
description Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses.
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spelling pubmed-49181622016-07-01 Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses Waggoner, Jesse J. Gresh, Lionel Mohamed-Hadley, Alisha Ballesteros, Gabriela Davila, Maria Jose Vargas Tellez, Yolanda Sahoo, Malaya K. Balmaseda, Angel Harris, Eva Pinsky, Benjamin A. Emerg Infect Dis Dispatch Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses. Centers for Disease Control and Prevention 2016-07 /pmc/articles/PMC4918162/ /pubmed/27184629 http://dx.doi.org/10.3201/eid2207.160326 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Waggoner, Jesse J.
Gresh, Lionel
Mohamed-Hadley, Alisha
Ballesteros, Gabriela
Davila, Maria Jose Vargas
Tellez, Yolanda
Sahoo, Malaya K.
Balmaseda, Angel
Harris, Eva
Pinsky, Benjamin A.
Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses
title Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses
title_full Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses
title_fullStr Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses
title_full_unstemmed Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses
title_short Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses
title_sort single-reaction multiplex reverse transcription pcr for detection of zika, chikungunya, and dengue viruses
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918162/
https://www.ncbi.nlm.nih.gov/pubmed/27184629
http://dx.doi.org/10.3201/eid2207.160326
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