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MART-10, a newly synthesized vitamin D analog, represses metastatic potential of head and neck squamous carcinoma cells

Even with multidisciplinary treatment, the prognosis and quality of life of patients diagnosed with head and neck squamous cell carcinoma (HNSCC) are still not satisfactory. Previously, 19-Nor-2α-(3-hydroxypropyl)-1α,25(OH)(2)D(3) (MART-10), the new brand 1α,25(OH)(2)D(3) analog, has been demonstrat...

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Autores principales: Yang, Shih-Wei, Tsai, Chi-Ying, Pan, Yi-Chun, Yeh, Chun-Nan, Pang, Jong-Hwei S, Takano, Masashi, Kittaka, Atsushi, Juang, Horng-Heng, Chen, Tai C, Chiang, Kun-Chun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918737/
https://www.ncbi.nlm.nih.gov/pubmed/27382252
http://dx.doi.org/10.2147/DDDT.S107256
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author Yang, Shih-Wei
Tsai, Chi-Ying
Pan, Yi-Chun
Yeh, Chun-Nan
Pang, Jong-Hwei S
Takano, Masashi
Kittaka, Atsushi
Juang, Horng-Heng
Chen, Tai C
Chiang, Kun-Chun
author_facet Yang, Shih-Wei
Tsai, Chi-Ying
Pan, Yi-Chun
Yeh, Chun-Nan
Pang, Jong-Hwei S
Takano, Masashi
Kittaka, Atsushi
Juang, Horng-Heng
Chen, Tai C
Chiang, Kun-Chun
author_sort Yang, Shih-Wei
collection PubMed
description Even with multidisciplinary treatment, the prognosis and quality of life of patients diagnosed with head and neck squamous cell carcinoma (HNSCC) are still not satisfactory. Previously, 19-Nor-2α-(3-hydroxypropyl)-1α,25(OH)(2)D(3) (MART-10), the new brand 1α,25(OH)(2)D(3) analog, has been demonstrated to be an effective drug to inhibit HNSCC growth in vitro. Since most cancer patients die of metastasis, in this study, the antimetastatic effect of MART-10 on HNSCC was investigated. Our results reveal that both 1α,25(OH)(2)D(3) and MART-10 effectively repressed the migration and invasion of HNSCC cells, with MART-10 being much more potent than 1α,25(OH)(2)D(3). The antimetastatic effect of 1α,25(OH)(2)D(3) and MART-10 was mediated by attenuation of epithelial–mesenchymal transition (EMT), which was supported by the finding that the expression of EMT-inducing transcriptional factors, Sail and Twist, was inhibited by 1α,25(OH)(2)D(3) and MART-10. The upregulation of E-cadherin and downregulation of N-cadherin in FaDu cells induced by both drugs further confirmed the repression of EMT. In addition, 1α,25(OH)(2)D(3) and MART-10 treatment inhibited intracellular MMP-9 expression and extracellular MMP activity in FaDu cells. Collectively, our results suggest that the less-calcemia 1α,25(OH)(2)D(3) analog, MART-10, is a promising drug for HNSCC treatment. Further clinical studies are warranted.
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spelling pubmed-49187372016-07-05 MART-10, a newly synthesized vitamin D analog, represses metastatic potential of head and neck squamous carcinoma cells Yang, Shih-Wei Tsai, Chi-Ying Pan, Yi-Chun Yeh, Chun-Nan Pang, Jong-Hwei S Takano, Masashi Kittaka, Atsushi Juang, Horng-Heng Chen, Tai C Chiang, Kun-Chun Drug Des Devel Ther Original Research Even with multidisciplinary treatment, the prognosis and quality of life of patients diagnosed with head and neck squamous cell carcinoma (HNSCC) are still not satisfactory. Previously, 19-Nor-2α-(3-hydroxypropyl)-1α,25(OH)(2)D(3) (MART-10), the new brand 1α,25(OH)(2)D(3) analog, has been demonstrated to be an effective drug to inhibit HNSCC growth in vitro. Since most cancer patients die of metastasis, in this study, the antimetastatic effect of MART-10 on HNSCC was investigated. Our results reveal that both 1α,25(OH)(2)D(3) and MART-10 effectively repressed the migration and invasion of HNSCC cells, with MART-10 being much more potent than 1α,25(OH)(2)D(3). The antimetastatic effect of 1α,25(OH)(2)D(3) and MART-10 was mediated by attenuation of epithelial–mesenchymal transition (EMT), which was supported by the finding that the expression of EMT-inducing transcriptional factors, Sail and Twist, was inhibited by 1α,25(OH)(2)D(3) and MART-10. The upregulation of E-cadherin and downregulation of N-cadherin in FaDu cells induced by both drugs further confirmed the repression of EMT. In addition, 1α,25(OH)(2)D(3) and MART-10 treatment inhibited intracellular MMP-9 expression and extracellular MMP activity in FaDu cells. Collectively, our results suggest that the less-calcemia 1α,25(OH)(2)D(3) analog, MART-10, is a promising drug for HNSCC treatment. Further clinical studies are warranted. Dove Medical Press 2016-06-17 /pmc/articles/PMC4918737/ /pubmed/27382252 http://dx.doi.org/10.2147/DDDT.S107256 Text en © 2016 Yang et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Yang, Shih-Wei
Tsai, Chi-Ying
Pan, Yi-Chun
Yeh, Chun-Nan
Pang, Jong-Hwei S
Takano, Masashi
Kittaka, Atsushi
Juang, Horng-Heng
Chen, Tai C
Chiang, Kun-Chun
MART-10, a newly synthesized vitamin D analog, represses metastatic potential of head and neck squamous carcinoma cells
title MART-10, a newly synthesized vitamin D analog, represses metastatic potential of head and neck squamous carcinoma cells
title_full MART-10, a newly synthesized vitamin D analog, represses metastatic potential of head and neck squamous carcinoma cells
title_fullStr MART-10, a newly synthesized vitamin D analog, represses metastatic potential of head and neck squamous carcinoma cells
title_full_unstemmed MART-10, a newly synthesized vitamin D analog, represses metastatic potential of head and neck squamous carcinoma cells
title_short MART-10, a newly synthesized vitamin D analog, represses metastatic potential of head and neck squamous carcinoma cells
title_sort mart-10, a newly synthesized vitamin d analog, represses metastatic potential of head and neck squamous carcinoma cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918737/
https://www.ncbi.nlm.nih.gov/pubmed/27382252
http://dx.doi.org/10.2147/DDDT.S107256
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