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Effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovine

BACKGROUND: Linoleic acid (LA) is a polyunsaturated fatty acid present in high concentrations in follicular fluid, when added to maturation culture media, it affects oocyte competence. OBJECTIVE: In the present study, we investigated effect of linoleic acid supplementation on in vitro maturation, em...

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Autores principales: Amini, Ebrahim, Asadpour, Reza, Roshangar, Leila, Jafari-Joozani, Razi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research and Clinical Center for Infertility 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918772/
https://www.ncbi.nlm.nih.gov/pubmed/27351027
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author Amini, Ebrahim
Asadpour, Reza
Roshangar, Leila
Jafari-Joozani, Razi
author_facet Amini, Ebrahim
Asadpour, Reza
Roshangar, Leila
Jafari-Joozani, Razi
author_sort Amini, Ebrahim
collection PubMed
description BACKGROUND: Linoleic acid (LA) is a polyunsaturated fatty acid present in high concentrations in follicular fluid, when added to maturation culture media, it affects oocyte competence. OBJECTIVE: In the present study, we investigated effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovine MATERIALS AND METHODS: The experiments conducted on 450 ovine Cumulus-oocyte complexes (COCs) with homogenous ooplasm and more than two compact layers of cumulus cells. For in vitro maturation COCs were randomly allocated into four treatment groups for 24 hr period. Treatment groups were as follow: control maturation media, 0 µM LA, 50 µM LA, 100 µM LA and 200 µM LA. The cumulus cell expansion and blastocysts rates were recorded. Total RNA was isolated from embryo pools, reverse transcribed into cDNA, and subjected to apoptotic gene expression by real-time PCR. RESULTS: Highest concentration (200 µM/mL) of LA significantly decreased the rate of fully expanded cumulus cells 24 hr after in vitro maturation (IVM) and the percentage of blastocyste rate compared with the control (p<0.05). These inhibitory effects were associated with an increased in relative mRNA expression of Bax (Bcl-2- associated X) gene compared with controls. CONCLUSION: Data obtained in present study suggest that low concentration of LA used for maturation had no deleterious effect on subsequent embryonic development compared to high concentration of LA. Relative expression of Bcl-2 (B-cell lymphoma 2) and Bax in embryos seems to be associated with LA concentration.
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spelling pubmed-49187722016-06-27 Effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovine Amini, Ebrahim Asadpour, Reza Roshangar, Leila Jafari-Joozani, Razi Int J Reprod Biomed Original Article BACKGROUND: Linoleic acid (LA) is a polyunsaturated fatty acid present in high concentrations in follicular fluid, when added to maturation culture media, it affects oocyte competence. OBJECTIVE: In the present study, we investigated effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovine MATERIALS AND METHODS: The experiments conducted on 450 ovine Cumulus-oocyte complexes (COCs) with homogenous ooplasm and more than two compact layers of cumulus cells. For in vitro maturation COCs were randomly allocated into four treatment groups for 24 hr period. Treatment groups were as follow: control maturation media, 0 µM LA, 50 µM LA, 100 µM LA and 200 µM LA. The cumulus cell expansion and blastocysts rates were recorded. Total RNA was isolated from embryo pools, reverse transcribed into cDNA, and subjected to apoptotic gene expression by real-time PCR. RESULTS: Highest concentration (200 µM/mL) of LA significantly decreased the rate of fully expanded cumulus cells 24 hr after in vitro maturation (IVM) and the percentage of blastocyste rate compared with the control (p<0.05). These inhibitory effects were associated with an increased in relative mRNA expression of Bax (Bcl-2- associated X) gene compared with controls. CONCLUSION: Data obtained in present study suggest that low concentration of LA used for maturation had no deleterious effect on subsequent embryonic development compared to high concentration of LA. Relative expression of Bcl-2 (B-cell lymphoma 2) and Bax in embryos seems to be associated with LA concentration. Research and Clinical Center for Infertility 2016-04 /pmc/articles/PMC4918772/ /pubmed/27351027 Text en http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Amini, Ebrahim
Asadpour, Reza
Roshangar, Leila
Jafari-Joozani, Razi
Effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovine
title Effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovine
title_full Effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovine
title_fullStr Effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovine
title_full_unstemmed Effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovine
title_short Effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovine
title_sort effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovine
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918772/
https://www.ncbi.nlm.nih.gov/pubmed/27351027
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