Optimization of Quantitative PCR Methods for Enteropathogen Detection

Detection and quantification of enteropathogens in stool specimens is useful for diagnosing the cause of diarrhea but is technically challenging. Here we evaluate several important determinants of quantification: specimen collection, nucleic acid extraction, and extraction and amplification efficien...

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Detalles Bibliográficos
Autores principales: Liu, Jie, Gratz, Jean, Amour, Caroline, Nshama, Rosemary, Walongo, Thomas, Maro, Athanasia, Mduma, Esto, Platts-Mills, James, Boisen, Nadia, Nataro, James, Haverstick, Doris M., Kabir, Furqan, Lertsethtakarn, Paphavee, Silapong, Sasikorn, Jeamwattanalert, Pimmada, Bodhidatta, Ladaporn, Mason, Carl, Begum, Sharmin, Haque, Rashidul, Praharaj, Ira, Kang, Gagandeep, Houpt, Eric R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918952/
https://www.ncbi.nlm.nih.gov/pubmed/27336160
http://dx.doi.org/10.1371/journal.pone.0158199

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