Production kinetics of polyhydroxyalkanoates by using Pseudomonas aeruginosa gamma ray mutant strain EBN-8 cultured on soybean oil

The purpose of present study was to optimize polyhydroxyalkanotes (PHAs) production in a gamma ray mutant strain of Pseudomonas aeruginosa grown on soybean oil in minimal salts media under shake flask conditions. The production kinetics was studied by sampling on daily basis for 6 days to investigat...

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Detalles Bibliográficos
Autores principales: Abid, Sharjeel, Raza, Zulfiqar Ali, Hussain, Tanveer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919136/
https://www.ncbi.nlm.nih.gov/pubmed/28330214
http://dx.doi.org/10.1007/s13205-016-0452-4
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author Abid, Sharjeel
Raza, Zulfiqar Ali
Hussain, Tanveer
author_facet Abid, Sharjeel
Raza, Zulfiqar Ali
Hussain, Tanveer
author_sort Abid, Sharjeel
collection PubMed
description The purpose of present study was to optimize polyhydroxyalkanotes (PHAs) production in a gamma ray mutant strain of Pseudomonas aeruginosa grown on soybean oil in minimal salts media under shake flask conditions. The production kinetics was studied by sampling on daily basis for 6 days to investigate the best conditions for PHAs production like biomass estimation, carbon source utilization and PHAs yield. The PHA accumulation was observed up to 50.27 % (w/w) of cell dry mass. The Pseudomonas species synthesized medium chain length PHA copolyester as per identified by LCMS and confirmed by FTIR spectroscopy. The ESI–MS analysis exhibited the major polyhydroxybutyrate with a molecular mass of m/z 448.5.
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spelling pubmed-49191362016-06-24 Production kinetics of polyhydroxyalkanoates by using Pseudomonas aeruginosa gamma ray mutant strain EBN-8 cultured on soybean oil Abid, Sharjeel Raza, Zulfiqar Ali Hussain, Tanveer 3 Biotech Original Article The purpose of present study was to optimize polyhydroxyalkanotes (PHAs) production in a gamma ray mutant strain of Pseudomonas aeruginosa grown on soybean oil in minimal salts media under shake flask conditions. The production kinetics was studied by sampling on daily basis for 6 days to investigate the best conditions for PHAs production like biomass estimation, carbon source utilization and PHAs yield. The PHA accumulation was observed up to 50.27 % (w/w) of cell dry mass. The Pseudomonas species synthesized medium chain length PHA copolyester as per identified by LCMS and confirmed by FTIR spectroscopy. The ESI–MS analysis exhibited the major polyhydroxybutyrate with a molecular mass of m/z 448.5. Springer Berlin Heidelberg 2016-06-23 2016-12 /pmc/articles/PMC4919136/ /pubmed/28330214 http://dx.doi.org/10.1007/s13205-016-0452-4 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Abid, Sharjeel
Raza, Zulfiqar Ali
Hussain, Tanveer
Production kinetics of polyhydroxyalkanoates by using Pseudomonas aeruginosa gamma ray mutant strain EBN-8 cultured on soybean oil
title Production kinetics of polyhydroxyalkanoates by using Pseudomonas aeruginosa gamma ray mutant strain EBN-8 cultured on soybean oil
title_full Production kinetics of polyhydroxyalkanoates by using Pseudomonas aeruginosa gamma ray mutant strain EBN-8 cultured on soybean oil
title_fullStr Production kinetics of polyhydroxyalkanoates by using Pseudomonas aeruginosa gamma ray mutant strain EBN-8 cultured on soybean oil
title_full_unstemmed Production kinetics of polyhydroxyalkanoates by using Pseudomonas aeruginosa gamma ray mutant strain EBN-8 cultured on soybean oil
title_short Production kinetics of polyhydroxyalkanoates by using Pseudomonas aeruginosa gamma ray mutant strain EBN-8 cultured on soybean oil
title_sort production kinetics of polyhydroxyalkanoates by using pseudomonas aeruginosa gamma ray mutant strain ebn-8 cultured on soybean oil
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919136/
https://www.ncbi.nlm.nih.gov/pubmed/28330214
http://dx.doi.org/10.1007/s13205-016-0452-4
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