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The Pepper CaOSR1 Protein Regulates the Osmotic Stress Response via Abscisic Acid Signaling

Plants are sessile organisms, and their growth and development is detrimentally affected by environmental stresses such as drought and high salinity. Defense mechanisms are tightly regulated and complex processes, which respond to changing environmental conditions; however, the precise mechanisms th...

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Autores principales: Park, Chanmi, Lim, Chae Woo, Lee, Sung Chul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919342/
https://www.ncbi.nlm.nih.gov/pubmed/27446121
http://dx.doi.org/10.3389/fpls.2016.00890
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author Park, Chanmi
Lim, Chae Woo
Lee, Sung Chul
author_facet Park, Chanmi
Lim, Chae Woo
Lee, Sung Chul
author_sort Park, Chanmi
collection PubMed
description Plants are sessile organisms, and their growth and development is detrimentally affected by environmental stresses such as drought and high salinity. Defense mechanisms are tightly regulated and complex processes, which respond to changing environmental conditions; however, the precise mechanisms that function under adverse conditions remain unclear. Here, we report the identification and functional characterization of the CaOSR1 gene, which functions in the adaptive response to abiotic stress. We found that CaOSR1 gene expression in pepper leaves was up-regulated after exposure to abscisic acid (ABA), drought, and high salinity. In addition, we demonstrated that the fusion protein of CaOSR1 with green fluorescent protein (GFP) is localized in the nucleus. We used CaOSR1-silenced pepper plants and CaOSR1-OX-overexpressing (OX) transgenic Arabidopsis plants to show that the CaOSR1 protein regulates the osmotic stress response. CaOSR1-silenced pepper plants showed increased drought susceptibility, and this was accompanied by a high transpiration rate. CaOSR1-OX plants displayed phenotypes that were hypersensitive to ABA and hyposensitive to osmotic stress, during the seed germination and seedling growth stages; furthermore, these plants exhibited enhanced drought tolerance at the adult stage, and this was characterized by higher leaf temperatures and smaller stomatal apertures because of ABA hypersensitivity. Taken together, our data indicate that CaOSR1 positively regulates osmotic stress tolerance via ABA-mediated cell signaling. These findings suggest an involvement of a novel protein in ABA and osmotic stress signalings in plants.
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spelling pubmed-49193422016-07-21 The Pepper CaOSR1 Protein Regulates the Osmotic Stress Response via Abscisic Acid Signaling Park, Chanmi Lim, Chae Woo Lee, Sung Chul Front Plant Sci Plant Science Plants are sessile organisms, and their growth and development is detrimentally affected by environmental stresses such as drought and high salinity. Defense mechanisms are tightly regulated and complex processes, which respond to changing environmental conditions; however, the precise mechanisms that function under adverse conditions remain unclear. Here, we report the identification and functional characterization of the CaOSR1 gene, which functions in the adaptive response to abiotic stress. We found that CaOSR1 gene expression in pepper leaves was up-regulated after exposure to abscisic acid (ABA), drought, and high salinity. In addition, we demonstrated that the fusion protein of CaOSR1 with green fluorescent protein (GFP) is localized in the nucleus. We used CaOSR1-silenced pepper plants and CaOSR1-OX-overexpressing (OX) transgenic Arabidopsis plants to show that the CaOSR1 protein regulates the osmotic stress response. CaOSR1-silenced pepper plants showed increased drought susceptibility, and this was accompanied by a high transpiration rate. CaOSR1-OX plants displayed phenotypes that were hypersensitive to ABA and hyposensitive to osmotic stress, during the seed germination and seedling growth stages; furthermore, these plants exhibited enhanced drought tolerance at the adult stage, and this was characterized by higher leaf temperatures and smaller stomatal apertures because of ABA hypersensitivity. Taken together, our data indicate that CaOSR1 positively regulates osmotic stress tolerance via ABA-mediated cell signaling. These findings suggest an involvement of a novel protein in ABA and osmotic stress signalings in plants. Frontiers Media S.A. 2016-06-24 /pmc/articles/PMC4919342/ /pubmed/27446121 http://dx.doi.org/10.3389/fpls.2016.00890 Text en Copyright © 2016 Park, Lim and Lee. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Park, Chanmi
Lim, Chae Woo
Lee, Sung Chul
The Pepper CaOSR1 Protein Regulates the Osmotic Stress Response via Abscisic Acid Signaling
title The Pepper CaOSR1 Protein Regulates the Osmotic Stress Response via Abscisic Acid Signaling
title_full The Pepper CaOSR1 Protein Regulates the Osmotic Stress Response via Abscisic Acid Signaling
title_fullStr The Pepper CaOSR1 Protein Regulates the Osmotic Stress Response via Abscisic Acid Signaling
title_full_unstemmed The Pepper CaOSR1 Protein Regulates the Osmotic Stress Response via Abscisic Acid Signaling
title_short The Pepper CaOSR1 Protein Regulates the Osmotic Stress Response via Abscisic Acid Signaling
title_sort pepper caosr1 protein regulates the osmotic stress response via abscisic acid signaling
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919342/
https://www.ncbi.nlm.nih.gov/pubmed/27446121
http://dx.doi.org/10.3389/fpls.2016.00890
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