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Gene Expression Profiling of Breast Cancer Brain Metastasis
The biology of breast cancer brain metastasis (BCBM) is poorly understood. We aimed to explore genes that are implicated in the process of brain metastasis of primary breast cancer (BC). NanoString nCounter Analysis covering 252 target genes was used for comparison of gene expression levels between...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919653/ https://www.ncbi.nlm.nih.gov/pubmed/27340107 http://dx.doi.org/10.1038/srep28623 |
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author | Lee, Ji Yun Park, Kyunghee Lee, Eunjin Ahn, TaeJin Jung, Hae Hyun Lim, Sung Hee Hong, Mineui Do, In-Gu Cho, Eun Yoon Kim, Duk-Hwan Kim, Ji-Yeon Ahn, Jin Seok Im, Young-Hyuck Park, Yeon Hee |
author_facet | Lee, Ji Yun Park, Kyunghee Lee, Eunjin Ahn, TaeJin Jung, Hae Hyun Lim, Sung Hee Hong, Mineui Do, In-Gu Cho, Eun Yoon Kim, Duk-Hwan Kim, Ji-Yeon Ahn, Jin Seok Im, Young-Hyuck Park, Yeon Hee |
author_sort | Lee, Ji Yun |
collection | PubMed |
description | The biology of breast cancer brain metastasis (BCBM) is poorly understood. We aimed to explore genes that are implicated in the process of brain metastasis of primary breast cancer (BC). NanoString nCounter Analysis covering 252 target genes was used for comparison of gene expression levels between 20 primary BCs that relapsed to brain and 41 BCBM samples. PAM50-based intrinsic subtypes such as HER2-enriched and basal-like were clearly over-represented in BCBM. A panel of 22 genes was found to be significantly differentially expressed between primary BC and BCBM. Five of these genes, CXCL12, MMP2, MMP11, VCAM1, and MME, which have previously been associated with tumor progression, angiogenesis, and metastasis, clearly discriminated between primary BC and BCBM. Notably, the five genes were significantly upregulated in primary BC compared to BCBM. Conversely, SOX2 and OLIG2 genes were upregulated in BCBM. These genes may participate in metastatic colonization but not in primary tumor development. Among patient-matched paired samples (n = 17), a PAM50 molecular subtype conversion was observed in eight cases (47.1%), with a trend toward unfavorable subtypes in patients with the distinct gene expression. Our findings, although not conclusive, reveal differentially expressed genes that might mediate the brain metastasis process. |
format | Online Article Text |
id | pubmed-4919653 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49196532016-06-28 Gene Expression Profiling of Breast Cancer Brain Metastasis Lee, Ji Yun Park, Kyunghee Lee, Eunjin Ahn, TaeJin Jung, Hae Hyun Lim, Sung Hee Hong, Mineui Do, In-Gu Cho, Eun Yoon Kim, Duk-Hwan Kim, Ji-Yeon Ahn, Jin Seok Im, Young-Hyuck Park, Yeon Hee Sci Rep Article The biology of breast cancer brain metastasis (BCBM) is poorly understood. We aimed to explore genes that are implicated in the process of brain metastasis of primary breast cancer (BC). NanoString nCounter Analysis covering 252 target genes was used for comparison of gene expression levels between 20 primary BCs that relapsed to brain and 41 BCBM samples. PAM50-based intrinsic subtypes such as HER2-enriched and basal-like were clearly over-represented in BCBM. A panel of 22 genes was found to be significantly differentially expressed between primary BC and BCBM. Five of these genes, CXCL12, MMP2, MMP11, VCAM1, and MME, which have previously been associated with tumor progression, angiogenesis, and metastasis, clearly discriminated between primary BC and BCBM. Notably, the five genes were significantly upregulated in primary BC compared to BCBM. Conversely, SOX2 and OLIG2 genes were upregulated in BCBM. These genes may participate in metastatic colonization but not in primary tumor development. Among patient-matched paired samples (n = 17), a PAM50 molecular subtype conversion was observed in eight cases (47.1%), with a trend toward unfavorable subtypes in patients with the distinct gene expression. Our findings, although not conclusive, reveal differentially expressed genes that might mediate the brain metastasis process. Nature Publishing Group 2016-06-24 /pmc/articles/PMC4919653/ /pubmed/27340107 http://dx.doi.org/10.1038/srep28623 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Lee, Ji Yun Park, Kyunghee Lee, Eunjin Ahn, TaeJin Jung, Hae Hyun Lim, Sung Hee Hong, Mineui Do, In-Gu Cho, Eun Yoon Kim, Duk-Hwan Kim, Ji-Yeon Ahn, Jin Seok Im, Young-Hyuck Park, Yeon Hee Gene Expression Profiling of Breast Cancer Brain Metastasis |
title | Gene Expression Profiling of Breast Cancer Brain Metastasis |
title_full | Gene Expression Profiling of Breast Cancer Brain Metastasis |
title_fullStr | Gene Expression Profiling of Breast Cancer Brain Metastasis |
title_full_unstemmed | Gene Expression Profiling of Breast Cancer Brain Metastasis |
title_short | Gene Expression Profiling of Breast Cancer Brain Metastasis |
title_sort | gene expression profiling of breast cancer brain metastasis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919653/ https://www.ncbi.nlm.nih.gov/pubmed/27340107 http://dx.doi.org/10.1038/srep28623 |
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