Nucleic Acid Templated Chemical Reaction in a Live Vertebrate

[Image: see text] Nucleic acid templated reactions are enabled by the hybridization of probe-reagent conjugates resulting in high effective reagent concentration and fast chemical transformation. We have developed a reaction that harnesses cellular microRNA (miRNA) to yield the cleavage of a linker...

Descripción completa

Detalles Bibliográficos
Autores principales: Holtzer, Laurent, Oleinich, Igor, Anzola, Marcello, Lindberg, Eric, Sadhu, Kalyan K., Gonzalez-Gaitan, Marcos, Winssinger, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2016
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919766/
https://www.ncbi.nlm.nih.gov/pubmed/27413783
http://dx.doi.org/10.1021/acscentsci.6b00054
_version_ 1782439297416167424
author Holtzer, Laurent
Oleinich, Igor
Anzola, Marcello
Lindberg, Eric
Sadhu, Kalyan K.
Gonzalez-Gaitan, Marcos
Winssinger, Nicolas
author_facet Holtzer, Laurent
Oleinich, Igor
Anzola, Marcello
Lindberg, Eric
Sadhu, Kalyan K.
Gonzalez-Gaitan, Marcos
Winssinger, Nicolas
author_sort Holtzer, Laurent
collection PubMed
description [Image: see text] Nucleic acid templated reactions are enabled by the hybridization of probe-reagent conjugates resulting in high effective reagent concentration and fast chemical transformation. We have developed a reaction that harnesses cellular microRNA (miRNA) to yield the cleavage of a linker releasing fluorogenic rhodamine in a live vertebrate. The reaction is based on the catalytic photoreduction of an azide by a ruthenium complex. We showed that this system reports specific expression of miRNA in living tissues of a vertebrate.
format Online
Article
Text
id pubmed-4919766
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-49197662016-07-13 Nucleic Acid Templated Chemical Reaction in a Live Vertebrate Holtzer, Laurent Oleinich, Igor Anzola, Marcello Lindberg, Eric Sadhu, Kalyan K. Gonzalez-Gaitan, Marcos Winssinger, Nicolas ACS Cent Sci [Image: see text] Nucleic acid templated reactions are enabled by the hybridization of probe-reagent conjugates resulting in high effective reagent concentration and fast chemical transformation. We have developed a reaction that harnesses cellular microRNA (miRNA) to yield the cleavage of a linker releasing fluorogenic rhodamine in a live vertebrate. The reaction is based on the catalytic photoreduction of an azide by a ruthenium complex. We showed that this system reports specific expression of miRNA in living tissues of a vertebrate. American Chemical Society 2016-05-18 2016-06-22 /pmc/articles/PMC4919766/ /pubmed/27413783 http://dx.doi.org/10.1021/acscentsci.6b00054 Text en Copyright © 2016 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Holtzer, Laurent
Oleinich, Igor
Anzola, Marcello
Lindberg, Eric
Sadhu, Kalyan K.
Gonzalez-Gaitan, Marcos
Winssinger, Nicolas
Nucleic Acid Templated Chemical Reaction in a Live Vertebrate
title Nucleic Acid Templated Chemical Reaction in a Live Vertebrate
title_full Nucleic Acid Templated Chemical Reaction in a Live Vertebrate
title_fullStr Nucleic Acid Templated Chemical Reaction in a Live Vertebrate
title_full_unstemmed Nucleic Acid Templated Chemical Reaction in a Live Vertebrate
title_short Nucleic Acid Templated Chemical Reaction in a Live Vertebrate
title_sort nucleic acid templated chemical reaction in a live vertebrate
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919766/
https://www.ncbi.nlm.nih.gov/pubmed/27413783
http://dx.doi.org/10.1021/acscentsci.6b00054
work_keys_str_mv AT holtzerlaurent nucleicacidtemplatedchemicalreactioninalivevertebrate
AT oleinichigor nucleicacidtemplatedchemicalreactioninalivevertebrate
AT anzolamarcello nucleicacidtemplatedchemicalreactioninalivevertebrate
AT lindbergeric nucleicacidtemplatedchemicalreactioninalivevertebrate
AT sadhukalyank nucleicacidtemplatedchemicalreactioninalivevertebrate
AT gonzalezgaitanmarcos nucleicacidtemplatedchemicalreactioninalivevertebrate
AT winssingernicolas nucleicacidtemplatedchemicalreactioninalivevertebrate

Ejemplares similares