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Different Effects of sgRNA Length on CRISPR-mediated Gene Knockout Efficiency

CRISPR-Cas9 is a powerful genome editing technology, yet with off-target effects. Truncated sgRNAs (17nt) have been found to decrease off-target cleavage without affecting on-target disruption in 293T cells. However, the potency of 17nt sgRNAs relative to the full-length 20nt sgRNAs in stem cells, s...

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Detalles Bibliográficos
Autores principales:	Zhang, Jian-Ping, Li, Xiao-Lan, Neises, Amanda, Chen, Wanqiu, Hu, Lin-Ping, Ji, Guang-Zhen, Yu, Jun-Yao, Xu, Jing, Yuan, Wei-Ping, Cheng, Tao, Zhang, Xiao-Bing
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919781/ https://www.ncbi.nlm.nih.gov/pubmed/27338021 http://dx.doi.org/10.1038/srep28566

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