Pathogenicity of reassortant H9 influenza viruses with different NA genes in mice and chickens

To better understand the influence of different NA genes on pathogenicity of H9 viruses, three reassortant H9 viruses (rH9N1, H9N2 and rH9N3) were generated and characterized. All three viruses replicated efficiently in eggs and MDCK cells, whereas the rH9N1 and rH9N3 replicated more efficiently tha...

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Autores principales: Yan, Liping, Liu, Qinfang, Su, Xin, Teng, Qiaoyang, Bao, Danqi, Che, Guangsheng, Chen, Hongjun, Cui, Hongrui, Ruan, Tao, Li, Xuesong, Li, Zejun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919838/
https://www.ncbi.nlm.nih.gov/pubmed/27342800
http://dx.doi.org/10.1186/s13567-016-0352-y
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author Yan, Liping
Liu, Qinfang
Su, Xin
Teng, Qiaoyang
Bao, Danqi
Che, Guangsheng
Chen, Hongjun
Cui, Hongrui
Ruan, Tao
Li, Xuesong
Li, Zejun
author_facet Yan, Liping
Liu, Qinfang
Su, Xin
Teng, Qiaoyang
Bao, Danqi
Che, Guangsheng
Chen, Hongjun
Cui, Hongrui
Ruan, Tao
Li, Xuesong
Li, Zejun
author_sort Yan, Liping
collection PubMed
description To better understand the influence of different NA genes on pathogenicity of H9 viruses, three reassortant H9 viruses (rH9N1, H9N2 and rH9N3) were generated and characterized. All three viruses replicated efficiently in eggs and MDCK cells, whereas the rH9N1 and rH9N3 replicated more efficiently than H9N2 in A549 cells. The rH9N3 replicated more efficiently than rH9N1 and H9N2 viruses in mice, however, rH9N3 replicated and shed less efficiently than the H9N2 virus in chickens. Further studies indicate that N3 had higher NA activity and released virus from erythrocytes faster, which may improve the adaptation of H9 influenza virus to mammals.
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spelling pubmed-49198382016-06-25 Pathogenicity of reassortant H9 influenza viruses with different NA genes in mice and chickens Yan, Liping Liu, Qinfang Su, Xin Teng, Qiaoyang Bao, Danqi Che, Guangsheng Chen, Hongjun Cui, Hongrui Ruan, Tao Li, Xuesong Li, Zejun Vet Res Short Report To better understand the influence of different NA genes on pathogenicity of H9 viruses, three reassortant H9 viruses (rH9N1, H9N2 and rH9N3) were generated and characterized. All three viruses replicated efficiently in eggs and MDCK cells, whereas the rH9N1 and rH9N3 replicated more efficiently than H9N2 in A549 cells. The rH9N3 replicated more efficiently than rH9N1 and H9N2 viruses in mice, however, rH9N3 replicated and shed less efficiently than the H9N2 virus in chickens. Further studies indicate that N3 had higher NA activity and released virus from erythrocytes faster, which may improve the adaptation of H9 influenza virus to mammals. BioMed Central 2016-06-24 2016 /pmc/articles/PMC4919838/ /pubmed/27342800 http://dx.doi.org/10.1186/s13567-016-0352-y Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Yan, Liping
Liu, Qinfang
Su, Xin
Teng, Qiaoyang
Bao, Danqi
Che, Guangsheng
Chen, Hongjun
Cui, Hongrui
Ruan, Tao
Li, Xuesong
Li, Zejun
Pathogenicity of reassortant H9 influenza viruses with different NA genes in mice and chickens
title Pathogenicity of reassortant H9 influenza viruses with different NA genes in mice and chickens
title_full Pathogenicity of reassortant H9 influenza viruses with different NA genes in mice and chickens
title_fullStr Pathogenicity of reassortant H9 influenza viruses with different NA genes in mice and chickens
title_full_unstemmed Pathogenicity of reassortant H9 influenza viruses with different NA genes in mice and chickens
title_short Pathogenicity of reassortant H9 influenza viruses with different NA genes in mice and chickens
title_sort pathogenicity of reassortant h9 influenza viruses with different na genes in mice and chickens
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919838/
https://www.ncbi.nlm.nih.gov/pubmed/27342800
http://dx.doi.org/10.1186/s13567-016-0352-y
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