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Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for rapid gene expression analysis

BACKGROUND: Phaseolus vulgaris is one of the most extensively studied model legumes in the world. The P. vulgaris genome sequence is available; therefore, the need for an efficient and rapid transformation system is more imperative than ever. The functional characterization of P. vulgaris genes is i...

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Detalles Bibliográficos
Autores principales:	Nanjareddy, Kalpana, Arthikala, Manoj-Kumar, Blanco, Lourdes, Arellano, Elizabeth S., Lara, Miguel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2016
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919892/ https://www.ncbi.nlm.nih.gov/pubmed/27342637 http://dx.doi.org/10.1186/s12896-016-0283-8

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