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Underestimation of ammonia‐oxidizing bacteria abundance by amplification bias in amoA‐targeted qPCR
Molecular methods to investigate functional groups in microbial communities rely on the specificity and selectivity of the primer set towards the target. Here, using rapid sand filters for drinking water production as model environment, we investigated the consistency of two commonly used quantitati...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919994/ https://www.ncbi.nlm.nih.gov/pubmed/27166579 http://dx.doi.org/10.1111/1751-7915.12366 |