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Isolation, partial purification and evaluation of bioactive compounds from leaves of Ageratum houstonianum
The present study deals with the isolation and partial purification of bioactive compounds from the crude methanol extracts of the leaves of Ageratum houstonianum (Asteraceae). The quantification and the identification of compounds in the crude extract and active bands isolated by preparative TLC we...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Leibniz Research Centre for Working Environment and Human Factors
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4920037/ https://www.ncbi.nlm.nih.gov/pubmed/27350770 |
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author | Zeeshan, M. Rizvi, S.M.D. Khan, M.S. Kumar, A. |
author_facet | Zeeshan, M. Rizvi, S.M.D. Khan, M.S. Kumar, A. |
author_sort | Zeeshan, M. |
collection | PubMed |
description | The present study deals with the isolation and partial purification of bioactive compounds from the crude methanol extracts of the leaves of Ageratum houstonianum (Asteraceae). The quantification and the identification of compounds in the crude extract and active bands isolated by preparative TLC were accomplished using GC-MS analysis. The most important compounds identified in the crude extract and active bands (AB-1 and AB-2) were 6-acetyl-7-methoxy-2, 2-dimethylchromene, hexadecanoic acid and squalene, respectively. Crude extract and active bands (AB-1 and AB-2) were investigated for their antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The crude extract, AB-1 and AB-2 showed maximum zone of inhibition (10-13 mm) against Staphylococcus epidermidis, however, the antibacterial potential of active bands was slightly higher as compared to the crude extract. Dose-dependant increase in antioxidant potential was noticed in crude extract as well as with both active bands measured by DPPH free radicals, ion chelation and total antioxidants capacity. Our study reports various bioactive compounds in the leaves of the A. houstonianum with significant antioxidant and antibacterial potential. |
format | Online Article Text |
id | pubmed-4920037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Leibniz Research Centre for Working Environment and Human Factors |
record_format | MEDLINE/PubMed |
spelling | pubmed-49200372016-06-27 Isolation, partial purification and evaluation of bioactive compounds from leaves of Ageratum houstonianum Zeeshan, M. Rizvi, S.M.D. Khan, M.S. Kumar, A. EXCLI J Original Article The present study deals with the isolation and partial purification of bioactive compounds from the crude methanol extracts of the leaves of Ageratum houstonianum (Asteraceae). The quantification and the identification of compounds in the crude extract and active bands isolated by preparative TLC were accomplished using GC-MS analysis. The most important compounds identified in the crude extract and active bands (AB-1 and AB-2) were 6-acetyl-7-methoxy-2, 2-dimethylchromene, hexadecanoic acid and squalene, respectively. Crude extract and active bands (AB-1 and AB-2) were investigated for their antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The crude extract, AB-1 and AB-2 showed maximum zone of inhibition (10-13 mm) against Staphylococcus epidermidis, however, the antibacterial potential of active bands was slightly higher as compared to the crude extract. Dose-dependant increase in antioxidant potential was noticed in crude extract as well as with both active bands measured by DPPH free radicals, ion chelation and total antioxidants capacity. Our study reports various bioactive compounds in the leaves of the A. houstonianum with significant antioxidant and antibacterial potential. Leibniz Research Centre for Working Environment and Human Factors 2012-02-28 /pmc/articles/PMC4920037/ /pubmed/27350770 Text en Copyright © 2012 Zeeshan et al. http://www.excli.de/documents/assignment_of_rights.pdf This is an Open Access article distributed under the following Assignment of Rights http://www.excli.de/documents/assignment_of_rights.pdf. You are free to copy, distribute and transmit the work, provided the original author and source are credited. |
spellingShingle | Original Article Zeeshan, M. Rizvi, S.M.D. Khan, M.S. Kumar, A. Isolation, partial purification and evaluation of bioactive compounds from leaves of Ageratum houstonianum |
title | Isolation, partial purification and evaluation of bioactive compounds from leaves of Ageratum houstonianum |
title_full | Isolation, partial purification and evaluation of bioactive compounds from leaves of Ageratum houstonianum |
title_fullStr | Isolation, partial purification and evaluation of bioactive compounds from leaves of Ageratum houstonianum |
title_full_unstemmed | Isolation, partial purification and evaluation of bioactive compounds from leaves of Ageratum houstonianum |
title_short | Isolation, partial purification and evaluation of bioactive compounds from leaves of Ageratum houstonianum |
title_sort | isolation, partial purification and evaluation of bioactive compounds from leaves of ageratum houstonianum |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4920037/ https://www.ncbi.nlm.nih.gov/pubmed/27350770 |
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