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Cadherin 2/4 signaling via PTP1B and catenins is crucial for nucleokinesis during radial neuronal migration in the neocortex

Cadherins are crucial for the radial migration of excitatory projection neurons into the developing neocortical wall. However, the specific cadherins and the signaling pathways that regulate radial migration are not well understood. Here, we show that cadherin 2 (CDH2) and CDH4 cooperate to regulate...

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Autores principales: Martinez-Garay, Isabel, Gil-Sanz, Cristina, Franco, Santos J., Espinosa, Ana, Molnár, Zoltán, Mueller, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4920171/
https://www.ncbi.nlm.nih.gov/pubmed/27151949
http://dx.doi.org/10.1242/dev.132456
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author Martinez-Garay, Isabel
Gil-Sanz, Cristina
Franco, Santos J.
Espinosa, Ana
Molnár, Zoltán
Mueller, Ulrich
author_facet Martinez-Garay, Isabel
Gil-Sanz, Cristina
Franco, Santos J.
Espinosa, Ana
Molnár, Zoltán
Mueller, Ulrich
author_sort Martinez-Garay, Isabel
collection PubMed
description Cadherins are crucial for the radial migration of excitatory projection neurons into the developing neocortical wall. However, the specific cadherins and the signaling pathways that regulate radial migration are not well understood. Here, we show that cadherin 2 (CDH2) and CDH4 cooperate to regulate radial migration in mouse brain via the protein tyrosine phosphatase 1B (PTP1B) and α- and β-catenins. Surprisingly, perturbation of cadherin-mediated signaling does not affect the formation and extension of leading processes of migrating neocortical neurons. Instead, movement of the cell body and nucleus (nucleokinesis) is disrupted. This defect is partially rescued by overexpression of LIS1, a microtubule-associated protein that has previously been shown to regulate nucleokinesis. Taken together, our findings indicate that cadherin-mediated signaling to the cytoskeleton is crucial for nucleokinesis of neocortical projection neurons during their radial migration.
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spelling pubmed-49201712016-07-25 Cadherin 2/4 signaling via PTP1B and catenins is crucial for nucleokinesis during radial neuronal migration in the neocortex Martinez-Garay, Isabel Gil-Sanz, Cristina Franco, Santos J. Espinosa, Ana Molnár, Zoltán Mueller, Ulrich Development Research Article Cadherins are crucial for the radial migration of excitatory projection neurons into the developing neocortical wall. However, the specific cadherins and the signaling pathways that regulate radial migration are not well understood. Here, we show that cadherin 2 (CDH2) and CDH4 cooperate to regulate radial migration in mouse brain via the protein tyrosine phosphatase 1B (PTP1B) and α- and β-catenins. Surprisingly, perturbation of cadherin-mediated signaling does not affect the formation and extension of leading processes of migrating neocortical neurons. Instead, movement of the cell body and nucleus (nucleokinesis) is disrupted. This defect is partially rescued by overexpression of LIS1, a microtubule-associated protein that has previously been shown to regulate nucleokinesis. Taken together, our findings indicate that cadherin-mediated signaling to the cytoskeleton is crucial for nucleokinesis of neocortical projection neurons during their radial migration. The Company of Biologists Ltd 2016-06-15 /pmc/articles/PMC4920171/ /pubmed/27151949 http://dx.doi.org/10.1242/dev.132456 Text en © 2016. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Martinez-Garay, Isabel
Gil-Sanz, Cristina
Franco, Santos J.
Espinosa, Ana
Molnár, Zoltán
Mueller, Ulrich
Cadherin 2/4 signaling via PTP1B and catenins is crucial for nucleokinesis during radial neuronal migration in the neocortex
title Cadherin 2/4 signaling via PTP1B and catenins is crucial for nucleokinesis during radial neuronal migration in the neocortex
title_full Cadherin 2/4 signaling via PTP1B and catenins is crucial for nucleokinesis during radial neuronal migration in the neocortex
title_fullStr Cadherin 2/4 signaling via PTP1B and catenins is crucial for nucleokinesis during radial neuronal migration in the neocortex
title_full_unstemmed Cadherin 2/4 signaling via PTP1B and catenins is crucial for nucleokinesis during radial neuronal migration in the neocortex
title_short Cadherin 2/4 signaling via PTP1B and catenins is crucial for nucleokinesis during radial neuronal migration in the neocortex
title_sort cadherin 2/4 signaling via ptp1b and catenins is crucial for nucleokinesis during radial neuronal migration in the neocortex
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4920171/
https://www.ncbi.nlm.nih.gov/pubmed/27151949
http://dx.doi.org/10.1242/dev.132456
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