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Frequency Responses of Rat Retinal Ganglion Cells

There are 15–20 different types of retinal ganglion cells (RGC) in the mammalian retina, each encoding different aspects of the visual scene. The mechanism by which post-synaptic signals from the retinal network generate spikes is determined by each cell’s intrinsic electrical properties. Here we in...

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Autores principales: Hadjinicolaou, Alex E., Cloherty, Shaun L., Hung, Yu-Shan, Kameneva, Tatiana, Ibbotson, Michael R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4920367/
https://www.ncbi.nlm.nih.gov/pubmed/27341669
http://dx.doi.org/10.1371/journal.pone.0157676
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author Hadjinicolaou, Alex E.
Cloherty, Shaun L.
Hung, Yu-Shan
Kameneva, Tatiana
Ibbotson, Michael R.
author_facet Hadjinicolaou, Alex E.
Cloherty, Shaun L.
Hung, Yu-Shan
Kameneva, Tatiana
Ibbotson, Michael R.
author_sort Hadjinicolaou, Alex E.
collection PubMed
description There are 15–20 different types of retinal ganglion cells (RGC) in the mammalian retina, each encoding different aspects of the visual scene. The mechanism by which post-synaptic signals from the retinal network generate spikes is determined by each cell’s intrinsic electrical properties. Here we investigate the frequency responses of morphologically identified rat RGCs using intracellular injection of sinusoidal current waveforms, to assess their intrinsic capabilities with minimal contributions from the retinal network. Recorded cells were classified according to their morphological characteristics (A, B, C or D-type) and their stratification (inner (i), outer (o) or bistratified) in the inner plexiform layer (IPL). Most cell types had low- or band-pass frequency responses. A2, C1 and C4o cells were band-pass with peaks of 15–30 Hz and low-pass cutoffs above 56 Hz (A2 cells) and ~42 Hz (C1 and C4o cells). A1 and C2i/o cells were low-pass with peaks of 10–15 Hz (cutoffs 19–25 Hz). Bistratified D1 and D2 cells were also low-pass with peaks of 5–10 Hz (cutoffs ~16 Hz). The least responsive cells were the B2 and C3 types (peaks: 2–5 Hz, cutoffs: 8–11 Hz). We found no difference between cells stratifying in the inner and outer IPL (i.e., ON and OFF cells) or between cells with large and small somas or dendritic fields. Intrinsic physiological properties (input resistance, spike width and sag) had little impact on frequency response at low frequencies, but account for 30–40% of response variability at frequencies >30 Hz.
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spelling pubmed-49203672016-07-18 Frequency Responses of Rat Retinal Ganglion Cells Hadjinicolaou, Alex E. Cloherty, Shaun L. Hung, Yu-Shan Kameneva, Tatiana Ibbotson, Michael R. PLoS One Research Article There are 15–20 different types of retinal ganglion cells (RGC) in the mammalian retina, each encoding different aspects of the visual scene. The mechanism by which post-synaptic signals from the retinal network generate spikes is determined by each cell’s intrinsic electrical properties. Here we investigate the frequency responses of morphologically identified rat RGCs using intracellular injection of sinusoidal current waveforms, to assess their intrinsic capabilities with minimal contributions from the retinal network. Recorded cells were classified according to their morphological characteristics (A, B, C or D-type) and their stratification (inner (i), outer (o) or bistratified) in the inner plexiform layer (IPL). Most cell types had low- or band-pass frequency responses. A2, C1 and C4o cells were band-pass with peaks of 15–30 Hz and low-pass cutoffs above 56 Hz (A2 cells) and ~42 Hz (C1 and C4o cells). A1 and C2i/o cells were low-pass with peaks of 10–15 Hz (cutoffs 19–25 Hz). Bistratified D1 and D2 cells were also low-pass with peaks of 5–10 Hz (cutoffs ~16 Hz). The least responsive cells were the B2 and C3 types (peaks: 2–5 Hz, cutoffs: 8–11 Hz). We found no difference between cells stratifying in the inner and outer IPL (i.e., ON and OFF cells) or between cells with large and small somas or dendritic fields. Intrinsic physiological properties (input resistance, spike width and sag) had little impact on frequency response at low frequencies, but account for 30–40% of response variability at frequencies >30 Hz. Public Library of Science 2016-06-24 /pmc/articles/PMC4920367/ /pubmed/27341669 http://dx.doi.org/10.1371/journal.pone.0157676 Text en © 2016 Hadjinicolaou et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hadjinicolaou, Alex E.
Cloherty, Shaun L.
Hung, Yu-Shan
Kameneva, Tatiana
Ibbotson, Michael R.
Frequency Responses of Rat Retinal Ganglion Cells
title Frequency Responses of Rat Retinal Ganglion Cells
title_full Frequency Responses of Rat Retinal Ganglion Cells
title_fullStr Frequency Responses of Rat Retinal Ganglion Cells
title_full_unstemmed Frequency Responses of Rat Retinal Ganglion Cells
title_short Frequency Responses of Rat Retinal Ganglion Cells
title_sort frequency responses of rat retinal ganglion cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4920367/
https://www.ncbi.nlm.nih.gov/pubmed/27341669
http://dx.doi.org/10.1371/journal.pone.0157676
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