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miR156a Mimic Represses the Epithelial–Mesenchymal Transition of Human Nasopharyngeal Cancer Cells by Targeting Junctional Adhesion Molecule A

MicroRNAs (miRNAs) have been documented as having an important role in the development of cancer. Broccoli is very popular in large groups of the population and has anticancer properties. Junctional adhesion molecule A (JAMA) is preferentially concentrated at tight junctions and influences cell morp...

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Autores principales: Tian, Yunhong, Cai, Longmei, Tian, Yunming, Tu, Yinuo, Qiu, Huizhi, Xie, Guofeng, Huang, Donglan, Zheng, Ronghui, Zhang, Weijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4920421/
https://www.ncbi.nlm.nih.gov/pubmed/27341697
http://dx.doi.org/10.1371/journal.pone.0157686
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author Tian, Yunhong
Cai, Longmei
Tian, Yunming
Tu, Yinuo
Qiu, Huizhi
Xie, Guofeng
Huang, Donglan
Zheng, Ronghui
Zhang, Weijun
author_facet Tian, Yunhong
Cai, Longmei
Tian, Yunming
Tu, Yinuo
Qiu, Huizhi
Xie, Guofeng
Huang, Donglan
Zheng, Ronghui
Zhang, Weijun
author_sort Tian, Yunhong
collection PubMed
description MicroRNAs (miRNAs) have been documented as having an important role in the development of cancer. Broccoli is very popular in large groups of the population and has anticancer properties. Junctional adhesion molecule A (JAMA) is preferentially concentrated at tight junctions and influences cell morphology and migration. Epithelial–mesenchymal transition (EMT) is a developmental program associated with cancer progression and metastasis. In this study we aimed to investigate the role of miRNAs from broccoli in human nasopharyngeal cancer (NPC). We demonstrated that a total of 84 conserved miRNAs and 184 putative novel miRNAs were found in broccoli by sequencing technology. Among these, miR156a was expressed the most. In addition, synthetic miR156a mimic inhibited the EMT of NPC cells in vitro. Furthermore, it was confirmed that JAMA was the target of miR156a mimic as validated by 3’ UTR luciferase reporter assays and western blotting. Knockdown of JAMA was consistent with the effects of miR156a mimic on the EMT of NPC, and the up-regulation of JAMA could partially restore EMT repressed by miR156a mimic. In conclusion, these results indicate that the miR156a mimic inhibits the EMT of NPC cells by targeting the 3’ UTR of JAMA. These miRNA profiles of broccoli provide a fundamental basis for further research. Moreover, the discovery of miR156a may have clinical implications for the treatment of patients with NPC.
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spelling pubmed-49204212016-07-18 miR156a Mimic Represses the Epithelial–Mesenchymal Transition of Human Nasopharyngeal Cancer Cells by Targeting Junctional Adhesion Molecule A Tian, Yunhong Cai, Longmei Tian, Yunming Tu, Yinuo Qiu, Huizhi Xie, Guofeng Huang, Donglan Zheng, Ronghui Zhang, Weijun PLoS One Research Article MicroRNAs (miRNAs) have been documented as having an important role in the development of cancer. Broccoli is very popular in large groups of the population and has anticancer properties. Junctional adhesion molecule A (JAMA) is preferentially concentrated at tight junctions and influences cell morphology and migration. Epithelial–mesenchymal transition (EMT) is a developmental program associated with cancer progression and metastasis. In this study we aimed to investigate the role of miRNAs from broccoli in human nasopharyngeal cancer (NPC). We demonstrated that a total of 84 conserved miRNAs and 184 putative novel miRNAs were found in broccoli by sequencing technology. Among these, miR156a was expressed the most. In addition, synthetic miR156a mimic inhibited the EMT of NPC cells in vitro. Furthermore, it was confirmed that JAMA was the target of miR156a mimic as validated by 3’ UTR luciferase reporter assays and western blotting. Knockdown of JAMA was consistent with the effects of miR156a mimic on the EMT of NPC, and the up-regulation of JAMA could partially restore EMT repressed by miR156a mimic. In conclusion, these results indicate that the miR156a mimic inhibits the EMT of NPC cells by targeting the 3’ UTR of JAMA. These miRNA profiles of broccoli provide a fundamental basis for further research. Moreover, the discovery of miR156a may have clinical implications for the treatment of patients with NPC. Public Library of Science 2016-06-24 /pmc/articles/PMC4920421/ /pubmed/27341697 http://dx.doi.org/10.1371/journal.pone.0157686 Text en © 2016 Tian et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Tian, Yunhong
Cai, Longmei
Tian, Yunming
Tu, Yinuo
Qiu, Huizhi
Xie, Guofeng
Huang, Donglan
Zheng, Ronghui
Zhang, Weijun
miR156a Mimic Represses the Epithelial–Mesenchymal Transition of Human Nasopharyngeal Cancer Cells by Targeting Junctional Adhesion Molecule A
title miR156a Mimic Represses the Epithelial–Mesenchymal Transition of Human Nasopharyngeal Cancer Cells by Targeting Junctional Adhesion Molecule A
title_full miR156a Mimic Represses the Epithelial–Mesenchymal Transition of Human Nasopharyngeal Cancer Cells by Targeting Junctional Adhesion Molecule A
title_fullStr miR156a Mimic Represses the Epithelial–Mesenchymal Transition of Human Nasopharyngeal Cancer Cells by Targeting Junctional Adhesion Molecule A
title_full_unstemmed miR156a Mimic Represses the Epithelial–Mesenchymal Transition of Human Nasopharyngeal Cancer Cells by Targeting Junctional Adhesion Molecule A
title_short miR156a Mimic Represses the Epithelial–Mesenchymal Transition of Human Nasopharyngeal Cancer Cells by Targeting Junctional Adhesion Molecule A
title_sort mir156a mimic represses the epithelial–mesenchymal transition of human nasopharyngeal cancer cells by targeting junctional adhesion molecule a
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4920421/
https://www.ncbi.nlm.nih.gov/pubmed/27341697
http://dx.doi.org/10.1371/journal.pone.0157686
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