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Non-target impact of fungicide tetraconazole on microbial communities in soils with different agricultural management

Effect of the fungicide tetraconazole on microbial community in silt loam soils from orchard with long history of triazole application and from grassland with no known history of fungicide usage was investigated. Triazole tetraconazole that had never been used on these soils before was applied at th...

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Autores principales: Sułowicz, Sławomir, Cycoń, Mariusz, Piotrowska-Seget, Zofia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4921116/
https://www.ncbi.nlm.nih.gov/pubmed/27106012
http://dx.doi.org/10.1007/s10646-016-1661-7
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author Sułowicz, Sławomir
Cycoń, Mariusz
Piotrowska-Seget, Zofia
author_facet Sułowicz, Sławomir
Cycoń, Mariusz
Piotrowska-Seget, Zofia
author_sort Sułowicz, Sławomir
collection PubMed
description Effect of the fungicide tetraconazole on microbial community in silt loam soils from orchard with long history of triazole application and from grassland with no known history of fungicide usage was investigated. Triazole tetraconazole that had never been used on these soils before was applied at the field rate and at tenfold the FR. Response of microbial communities to tetraconazole was investigated during 28-day laboratory experiment by determination of changes in their biomass and structure (phospholipid fatty acids method—PLFA), activity (fluorescein diacetate hydrolysis—FDA) as well as changes in genetic (DGGE) and functional (Biolog) diversity. Obtained results indicated that the response of soil microorganisms to tetraconazole depended on the management of the soils. DGGE patterns revealed that both dosages of fungicide affected the structure of bacterial community and the impact on genetic diversity and richness was more prominent in orchard soil. Values of stress indices—the saturated/monounsaturated PLFAs ratio and the cyclo/monounsaturated precursors ratio, were almost twice as high and the Gram-negative/Gram-positive ratio was significantly lower in the orchard soil compared with the grassland soil. Results of principal component analysis of PLFA and Biolog profiles revealed significant impact of tetraconazole in orchard soil on day 28, whereas changes in these profiles obtained for grassland soil were insignificant or transient. Obtained results indicated that orchards soil seems to be more vulnerable to tetraconazole application compared to grassland soil. History of pesticide application and agricultural management should be taken into account in assessing of environmental impact of studied pesticides. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10646-016-1661-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-49211162016-07-12 Non-target impact of fungicide tetraconazole on microbial communities in soils with different agricultural management Sułowicz, Sławomir Cycoń, Mariusz Piotrowska-Seget, Zofia Ecotoxicology Article Effect of the fungicide tetraconazole on microbial community in silt loam soils from orchard with long history of triazole application and from grassland with no known history of fungicide usage was investigated. Triazole tetraconazole that had never been used on these soils before was applied at the field rate and at tenfold the FR. Response of microbial communities to tetraconazole was investigated during 28-day laboratory experiment by determination of changes in their biomass and structure (phospholipid fatty acids method—PLFA), activity (fluorescein diacetate hydrolysis—FDA) as well as changes in genetic (DGGE) and functional (Biolog) diversity. Obtained results indicated that the response of soil microorganisms to tetraconazole depended on the management of the soils. DGGE patterns revealed that both dosages of fungicide affected the structure of bacterial community and the impact on genetic diversity and richness was more prominent in orchard soil. Values of stress indices—the saturated/monounsaturated PLFAs ratio and the cyclo/monounsaturated precursors ratio, were almost twice as high and the Gram-negative/Gram-positive ratio was significantly lower in the orchard soil compared with the grassland soil. Results of principal component analysis of PLFA and Biolog profiles revealed significant impact of tetraconazole in orchard soil on day 28, whereas changes in these profiles obtained for grassland soil were insignificant or transient. Obtained results indicated that orchards soil seems to be more vulnerable to tetraconazole application compared to grassland soil. History of pesticide application and agricultural management should be taken into account in assessing of environmental impact of studied pesticides. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10646-016-1661-7) contains supplementary material, which is available to authorized users. Springer US 2016-04-22 2016 /pmc/articles/PMC4921116/ /pubmed/27106012 http://dx.doi.org/10.1007/s10646-016-1661-7 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Sułowicz, Sławomir
Cycoń, Mariusz
Piotrowska-Seget, Zofia
Non-target impact of fungicide tetraconazole on microbial communities in soils with different agricultural management
title Non-target impact of fungicide tetraconazole on microbial communities in soils with different agricultural management
title_full Non-target impact of fungicide tetraconazole on microbial communities in soils with different agricultural management
title_fullStr Non-target impact of fungicide tetraconazole on microbial communities in soils with different agricultural management
title_full_unstemmed Non-target impact of fungicide tetraconazole on microbial communities in soils with different agricultural management
title_short Non-target impact of fungicide tetraconazole on microbial communities in soils with different agricultural management
title_sort non-target impact of fungicide tetraconazole on microbial communities in soils with different agricultural management
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4921116/
https://www.ncbi.nlm.nih.gov/pubmed/27106012
http://dx.doi.org/10.1007/s10646-016-1661-7
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