Cargando…

Identifying Kinase Substrates via a Heavy ATP Kinase Assay and Quantitative Mass Spectrometry

Mass spectrometry-based in vitro kinase screens play an essential role in the discovery of kinase substrates, however, many suffer from biological and technical noise or necessitate genetically-altered enzyme-cofactor systems. We describe a method that combines stable γ-[(18)O(2)]-ATP with classical...

Descripción completa

Detalles Bibliográficos
Autores principales:	Müller, André C., Giambruno, Roberto, Weißer, Juliane, Májek, Peter, Hofer, Alexandre, Bigenzahn, Johannes W., Superti-Furga, Giulio, Jessen, Henning J., Bennett, Keiryn L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4921819/ https://www.ncbi.nlm.nih.gov/pubmed/27346722 http://dx.doi.org/10.1038/srep28107

Ejemplares similares

An Inducible Retroviral Expression System for Tandem Affinity Purification Mass-Spectrometry-Based Proteomics Identifies Mixed Lineage Kinase Domain-like Protein (MLKL) as an Heat Shock Protein 90 (HSP90) Client
por: Bigenzahn, Johannes W., et al.
Publicado: (2016)

Building and exploring an integrated human kinase network: Global organization and medical entry points()
por: Colinge, Jacques, et al.
Publicado: (2014)

Affinity Purification Strategies for Proteomic Analysis of Transcription Factor Complexes
por: Giambruno, Roberto, et al.
Publicado: (2013)

Recent advances in combinatorial drug screening and synergy scoring
por: Pemovska, Tea, et al.
Publicado: (2018)

The DEAD-box helicase DDX3X is a critical component of the TANK-binding kinase 1-dependent innate immune response
por: Soulat, Didier, et al.
Publicado: (2008)

Structural Coupling of SH2-Kinase Domains Links Fes and Abl Substrate Recognition and Kinase Activation
por: Filippakopoulos, Panagis, et al.
Publicado: (2008)

A Computational Approach to Analyze the Mechanism of Action of the Kinase Inhibitor Bafetinib
por: Burkard, Thomas R., et al.
Publicado: (2010)

Initial characterization of the human central proteome
por: Burkard, Thomas R, et al.
Publicado: (2011)

The SH2 Domain Regulates c-Abl Kinase Activation by a Cyclin-Like Mechanism and Remodulation of the Hinge Motion
por: Dölker, Nicole, et al.
Publicado: (2014)

Detection of Chemical Engagement of Solute Carrier Proteins by a Cellular Thermal Shift Assay
por: Hashimoto, Mari, et al.
Publicado: (2018)

Experimental characterization of the human non-sequence-specific nucleic acid interactome
por: Dürnberger, Gerhard, et al.
Publicado: (2013)

The lysine methyltransferase SMYD3 interacts with hepatitis C virus NS5A and is a negative regulator of viral particle production
por: Eberle, Carol-Ann, et al.
Publicado: (2014)

Proteome-wide small molecule and metabolite interaction mapping
por: Huber, Kilian V. M., et al.
Publicado: (2015)

Investigation of pyrophosphate versus ATP substrate selection in the Entamoeba histolytica acetate kinase
por: Dang, Thanh, et al.
Publicado: (2017)

A substrate‐based ontology for human solute carriers
por: Meixner, Eva, et al.
Publicado: (2020)

A time-resolved molecular map of the macrophage response to VSV infection
por: Kandasamy, Richard K, et al.
Publicado: (2016)

A potent and highly specific FN3 monobody inhibitor of the Abl SH2 domain
por: Wojcik, John, et al.
Publicado: (2010)

The phosphatase UBASH3B/Sts-1 is a negative regulator of Bcr-Abl kinase activity and leukemogenesis
por: Mian, Afsar A., et al.
Publicado: (2019)

Sustained activation of the AKT/mTOR and MAP kinase pathways mediate resistance to the Src inhibitor, dasatinib, in thyroid cancer
por: Mishall, Katie M., et al.
Publicado: (2017)

Cellular Ser/Thr-Kinase Assays Using Generic Peptide Substrates
por: Adams, Deanna G, et al.
Publicado: (2008)

Semi-Automated High-Throughput Substrate Screening Assay for Nucleoside Kinases
por: Hellendahl, Katja F., et al.
Publicado: (2021)

Structures of Fission Yeast Inositol Pyrophosphate Kinase Asp1 in Ligand-Free, Substrate-Bound, and Product-Bound States
por: Benjamin, Bradley, et al.
Publicado: (2022)

Cell biology: A key driver of therapeutic innovation
por: Hantschel, Oliver, et al.
Publicado: (2012)

SLC38A9: A lysosomal amino acid transporter at the core of the amino acid-sensing machinery that controls MTORC1
por: Rebsamen, Manuele, et al.
Publicado: (2015)

A structure and evolutionary-based classification of solute carriers
por: Ferrada, Evandro, et al.
Publicado: (2022)

Identification of c-Src Tyrosine Kinase Substrates Using Mass Spectrometry and Peptide Microarrays
por: Amanchy, Ramars, et al.
Publicado: (2008)

Internalization of Pseudomonas aeruginosa Strain PAO1 into Epithelial Cells Is Promoted by Interaction of a T6SS Effector with the Microtubule Network
por: Sana, Thibault G., et al.
Publicado: (2015)

Targeting the SH2-Kinase Interface in Bcr-Abl Inhibits Leukemogenesis
por: Grebien, Florian, et al.
Publicado: (2011)

FLEXIQinase, a mass spectrometry-based assay, to unveil multi-kinase mechanisms
por: Singh, Sasha, et al.
Publicado: (2012)

Targeted mass-spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP kinase, and AKT signaling
por: Whiteaker, Jeffrey R., et al.
Publicado: (2021)

Kinase-interacting substrate screening is a novel method to identify kinase substrates
por: Amano, Mutsuki, et al.
Publicado: (2015)

ATP-competitive inhibitors modulate the substrate binding cooperativity of a kinase by altering its conformational entropy
por: Olivieri, Cristina, et al.
Publicado: (2022)

High-throughput kinase assays with protein substrates using fluorescent polymer superquenching
por: Rininsland, Frauke, et al.
Publicado: (2005)

The role of the C8 proton of ATP in the catalysis of shikimate kinase and adenylate kinase
por: Kenyon, Colin P, et al.
Publicado: (2012)

Metabolic drug survey highlights cancer cell dependencies and vulnerabilities
por: Pemovska, Tea, et al.
Publicado: (2021)

A chemical and phosphoproteomic characterization of dasatinib action in lung cancer
por: Li, Jiannong, et al.
Publicado: (2010)

Exploiting the Unique ATP-Binding Pocket of Toxoplasma Calcium-Dependent Protein Kinase 1 To Identify Its Substrates
por: Lourido, Sebastian, et al.
Publicado: (2013)

A computational protocol to evaluate the effects of protein mutants in the kinase gatekeeper position on the binding of ATP substrate analogues
por: Romano, Valentina, et al.
Publicado: (2017)

Kinase-Associated Phosphoisoform Assay: a novel candidate-based method to detect specific kinase-substrate phosphorylation interactions in vivo
por: Dory, Magdalena, et al.
Publicado: (2016)

Targeting allosteric regulatory modules in oncoproteins: “Drugging the Undruggable”
por: Hantschel, Oliver, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_kneu12ut1of4q9tcg8i6r2vbp3