Autophagy Gene Activity May Act As a Key Factor for Sensitivity of Tumor Cells to Oncolytic Vesicular Stomatitis Virus

BACKGROUND: Beclin1 is an important, primary molecule for autophagy. OBJECTIVES: It is suggested that the control of the autophagy path increases the sensitivity of tumor cells to VSV. MATERIALS AND METHODS: In this study, the degree of Beclin1 gene expression in two cell lines, HeLa and A549, has b...

Descripción completa

Detalles Bibliográficos
Autores principales: Nabizadeh, Arezoo, Bamdad, Taravat, Arefian, Ehsan, Razavi Nikoo, Seyed Hadi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shahid Beheshti University of Medical Sciences 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4922204/
https://www.ncbi.nlm.nih.gov/pubmed/27366311
http://dx.doi.org/10.17795/ijcp-3919
_version_ 1782439580335603712
author Nabizadeh, Arezoo
Bamdad, Taravat
Arefian, Ehsan
Razavi Nikoo, Seyed Hadi
author_facet Nabizadeh, Arezoo
Bamdad, Taravat
Arefian, Ehsan
Razavi Nikoo, Seyed Hadi
author_sort Nabizadeh, Arezoo
collection PubMed
description BACKGROUND: Beclin1 is an important, primary molecule for autophagy. OBJECTIVES: It is suggested that the control of the autophagy path increases the sensitivity of tumor cells to VSV. MATERIALS AND METHODS: In this study, the degree of Beclin1 gene expression in two cell lines, HeLa and A549, has been examined and the percentage of living cells subsequent infection with virus has been evaluated by MTT assay method. RESULTS: The results showed that the degree of Beclin1 gene expression in HeLa cells in comparison with A549 cells has reduced, and the sensitivity of these cells to vesicular stomatits virus (VSV) oncolysis is more than A549. CONCLUSIONS: It seems that by using some methods for reducing autophagy, it is possible to make tumor cells more sensitive to virotherapy and even other treatments.
format Online
Article
Text
id pubmed-4922204
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Shahid Beheshti University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-49222042016-06-30 Autophagy Gene Activity May Act As a Key Factor for Sensitivity of Tumor Cells to Oncolytic Vesicular Stomatitis Virus Nabizadeh, Arezoo Bamdad, Taravat Arefian, Ehsan Razavi Nikoo, Seyed Hadi Iran J Cancer Prev Research Article BACKGROUND: Beclin1 is an important, primary molecule for autophagy. OBJECTIVES: It is suggested that the control of the autophagy path increases the sensitivity of tumor cells to VSV. MATERIALS AND METHODS: In this study, the degree of Beclin1 gene expression in two cell lines, HeLa and A549, has been examined and the percentage of living cells subsequent infection with virus has been evaluated by MTT assay method. RESULTS: The results showed that the degree of Beclin1 gene expression in HeLa cells in comparison with A549 cells has reduced, and the sensitivity of these cells to vesicular stomatits virus (VSV) oncolysis is more than A549. CONCLUSIONS: It seems that by using some methods for reducing autophagy, it is possible to make tumor cells more sensitive to virotherapy and even other treatments. Shahid Beheshti University of Medical Sciences 2016-02-22 /pmc/articles/PMC4922204/ /pubmed/27366311 http://dx.doi.org/10.17795/ijcp-3919 Text en Copyright © 2016, Iranian Journal of Cancer Prevention. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Research Article
Nabizadeh, Arezoo
Bamdad, Taravat
Arefian, Ehsan
Razavi Nikoo, Seyed Hadi
Autophagy Gene Activity May Act As a Key Factor for Sensitivity of Tumor Cells to Oncolytic Vesicular Stomatitis Virus
title Autophagy Gene Activity May Act As a Key Factor for Sensitivity of Tumor Cells to Oncolytic Vesicular Stomatitis Virus
title_full Autophagy Gene Activity May Act As a Key Factor for Sensitivity of Tumor Cells to Oncolytic Vesicular Stomatitis Virus
title_fullStr Autophagy Gene Activity May Act As a Key Factor for Sensitivity of Tumor Cells to Oncolytic Vesicular Stomatitis Virus
title_full_unstemmed Autophagy Gene Activity May Act As a Key Factor for Sensitivity of Tumor Cells to Oncolytic Vesicular Stomatitis Virus
title_short Autophagy Gene Activity May Act As a Key Factor for Sensitivity of Tumor Cells to Oncolytic Vesicular Stomatitis Virus
title_sort autophagy gene activity may act as a key factor for sensitivity of tumor cells to oncolytic vesicular stomatitis virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4922204/
https://www.ncbi.nlm.nih.gov/pubmed/27366311
http://dx.doi.org/10.17795/ijcp-3919
work_keys_str_mv AT nabizadeharezoo autophagygeneactivitymayactasakeyfactorforsensitivityoftumorcellstooncolyticvesicularstomatitisvirus
AT bamdadtaravat autophagygeneactivitymayactasakeyfactorforsensitivityoftumorcellstooncolyticvesicularstomatitisvirus
AT arefianehsan autophagygeneactivitymayactasakeyfactorforsensitivityoftumorcellstooncolyticvesicularstomatitisvirus
AT razavinikooseyedhadi autophagygeneactivitymayactasakeyfactorforsensitivityoftumorcellstooncolyticvesicularstomatitisvirus

Ejemplares similares