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The Effects of High Fat Diet and Resveratrol on Mitochondrial Activity of Brown Adipocytes

BACKGROUND: Resveratrol (RSV) is a polyphenolic phytoalexin that has many effects on metabolic diseases such as diabetes and obesity. Given the importance of brown adipose tissue (BAT) for energy expenditure, we investigated the effects of RSV on brown adipocytes. METHODS: For the in vitro study, in...

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Autores principales: Ku, Cheol Ryong, Cho, Yoon Hee, Hong, Zhen-Yu, Lee, Ha, Lee, Sue Ji, Hong, Seung-soo, Lee, Eun Jig
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Endocrine Society 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4923418/
https://www.ncbi.nlm.nih.gov/pubmed/27077216
http://dx.doi.org/10.3803/EnM.2016.31.2.328
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author Ku, Cheol Ryong
Cho, Yoon Hee
Hong, Zhen-Yu
Lee, Ha
Lee, Sue Ji
Hong, Seung-soo
Lee, Eun Jig
author_facet Ku, Cheol Ryong
Cho, Yoon Hee
Hong, Zhen-Yu
Lee, Ha
Lee, Sue Ji
Hong, Seung-soo
Lee, Eun Jig
author_sort Ku, Cheol Ryong
collection PubMed
description BACKGROUND: Resveratrol (RSV) is a polyphenolic phytoalexin that has many effects on metabolic diseases such as diabetes and obesity. Given the importance of brown adipose tissue (BAT) for energy expenditure, we investigated the effects of RSV on brown adipocytes. METHODS: For the in vitro study, interscapular BAT was isolated from 7-week-old male Sprague Dawley rats. For the in vivo study, 7-week-old male Otsuka Long Evans Tokushima Fatty (OLETF) rats were divided into four groups and treated for 27 weeks with: standard diet (SD); SD+RSV (10 mg/kg body weight, daily); high fat diet (HFD); HFD+RSV. RSV was provided via oral gavage once daily during the in vivo experiments. RESULTS: RSV treatment of primary cultured brown preadipocytes promoted mitochondrial activity, along with over-expression of estrogen receptor α (ER-α). In OLETF rats, both HFD and RSV treatment increased the weight of BAT and the differentiation of BAT. However, only RSV increased the mitochondrial activity and ER-α expression of BAT in the HFD-fed group. Finally, RSV improved the insulin sensitivity of OLETF rats by increasing the mitochondrial activity of BAT, despite having no effects on white adipocytes and muscles in either diet group. CONCLUSION: RSV could improve insulin resistance, which might be associated with mitochondrial activity of brown adipocyte. Further studies evaluating the activity of RSV for both the differentiation and mitochondrial activity of BAT could be helpful in investigating the effects of RSV on metabolic parameters.
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spelling pubmed-49234182016-07-07 The Effects of High Fat Diet and Resveratrol on Mitochondrial Activity of Brown Adipocytes Ku, Cheol Ryong Cho, Yoon Hee Hong, Zhen-Yu Lee, Ha Lee, Sue Ji Hong, Seung-soo Lee, Eun Jig Endocrinol Metab (Seoul) Original Article BACKGROUND: Resveratrol (RSV) is a polyphenolic phytoalexin that has many effects on metabolic diseases such as diabetes and obesity. Given the importance of brown adipose tissue (BAT) for energy expenditure, we investigated the effects of RSV on brown adipocytes. METHODS: For the in vitro study, interscapular BAT was isolated from 7-week-old male Sprague Dawley rats. For the in vivo study, 7-week-old male Otsuka Long Evans Tokushima Fatty (OLETF) rats were divided into four groups and treated for 27 weeks with: standard diet (SD); SD+RSV (10 mg/kg body weight, daily); high fat diet (HFD); HFD+RSV. RSV was provided via oral gavage once daily during the in vivo experiments. RESULTS: RSV treatment of primary cultured brown preadipocytes promoted mitochondrial activity, along with over-expression of estrogen receptor α (ER-α). In OLETF rats, both HFD and RSV treatment increased the weight of BAT and the differentiation of BAT. However, only RSV increased the mitochondrial activity and ER-α expression of BAT in the HFD-fed group. Finally, RSV improved the insulin sensitivity of OLETF rats by increasing the mitochondrial activity of BAT, despite having no effects on white adipocytes and muscles in either diet group. CONCLUSION: RSV could improve insulin resistance, which might be associated with mitochondrial activity of brown adipocyte. Further studies evaluating the activity of RSV for both the differentiation and mitochondrial activity of BAT could be helpful in investigating the effects of RSV on metabolic parameters. Korean Endocrine Society 2016-06 2016-04-08 /pmc/articles/PMC4923418/ /pubmed/27077216 http://dx.doi.org/10.3803/EnM.2016.31.2.328 Text en Copyright © 2016 Korean Endocrine Society http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ku, Cheol Ryong
Cho, Yoon Hee
Hong, Zhen-Yu
Lee, Ha
Lee, Sue Ji
Hong, Seung-soo
Lee, Eun Jig
The Effects of High Fat Diet and Resveratrol on Mitochondrial Activity of Brown Adipocytes
title The Effects of High Fat Diet and Resveratrol on Mitochondrial Activity of Brown Adipocytes
title_full The Effects of High Fat Diet and Resveratrol on Mitochondrial Activity of Brown Adipocytes
title_fullStr The Effects of High Fat Diet and Resveratrol on Mitochondrial Activity of Brown Adipocytes
title_full_unstemmed The Effects of High Fat Diet and Resveratrol on Mitochondrial Activity of Brown Adipocytes
title_short The Effects of High Fat Diet and Resveratrol on Mitochondrial Activity of Brown Adipocytes
title_sort effects of high fat diet and resveratrol on mitochondrial activity of brown adipocytes
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4923418/
https://www.ncbi.nlm.nih.gov/pubmed/27077216
http://dx.doi.org/10.3803/EnM.2016.31.2.328
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