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Biomimetic Synthesis of Silver Nanoparticles Using Endosymbiotic Bacterium Inhabiting Euphorbia hirta L. and Their Bactericidal Potential
The present investigation aims to evaluate biomimetic synthesis of silver nanoparticles using endophytic bacterium EH 419 inhabiting Euphorbia hirta L. The synthesized nanoparticles were initially confirmed with change in color from the reaction mixture to brown indicating the synthesis of nanoparti...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4923596/ https://www.ncbi.nlm.nih.gov/pubmed/27403378 http://dx.doi.org/10.1155/2016/9020239 |
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author | Syed, Baker Yashavantha Rao, Hoovinakola Chinnappa Nagendra-Prasad, Mysore Nagalingaswamy Prasad, Ashwini Harini, Ballagere Puttaraju Azmath, Pasha Rakshith, Devaraju Satish, Sreedharamurthy |
author_facet | Syed, Baker Yashavantha Rao, Hoovinakola Chinnappa Nagendra-Prasad, Mysore Nagalingaswamy Prasad, Ashwini Harini, Ballagere Puttaraju Azmath, Pasha Rakshith, Devaraju Satish, Sreedharamurthy |
author_sort | Syed, Baker |
collection | PubMed |
description | The present investigation aims to evaluate biomimetic synthesis of silver nanoparticles using endophytic bacterium EH 419 inhabiting Euphorbia hirta L. The synthesized nanoparticles were initially confirmed with change in color from the reaction mixture to brown indicating the synthesis of nanoparticles. Further confirmation was achieved with the characteristic absorption peak at 440 nm using UV-Visible spectroscopy. The synthesized silver nanoparticles were subjected to biophysical characterization using hyphenated techniques. The possible role of biomolecules in mediating the synthesis was depicted with FTIR analysis. Further crystalline nature of synthesized nanoparticles was confirmed using X-ray diffraction (XRD) with prominent diffraction peaks at 2θ which can be indexed to the (111), (200), (220), and (311) reflections of face centered cubic structure (fcc) of metallic silver. Transmission electron microscopy (TEM) revealed morphological characteristics of synthesized silver nanoparticles to be polydisperse in nature with size ranging from 10 to 60 nm and different morphological characteristics such as spherical, oval, hexagonal, and cubic shapes. Further silver nanoparticles exhibited bactericidal activity against panel of significant pathogenic bacteria among which Pseudomonas aeruginosa was most sensitive compared to other pathogens. To the best of our knowledge, present study forms first report of bacterial endophyte inhabiting Euphorbia hirta L. in mediating synthesizing silver nanoparticles. |
format | Online Article Text |
id | pubmed-4923596 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-49235962016-07-11 Biomimetic Synthesis of Silver Nanoparticles Using Endosymbiotic Bacterium Inhabiting Euphorbia hirta L. and Their Bactericidal Potential Syed, Baker Yashavantha Rao, Hoovinakola Chinnappa Nagendra-Prasad, Mysore Nagalingaswamy Prasad, Ashwini Harini, Ballagere Puttaraju Azmath, Pasha Rakshith, Devaraju Satish, Sreedharamurthy Scientifica (Cairo) Research Article The present investigation aims to evaluate biomimetic synthesis of silver nanoparticles using endophytic bacterium EH 419 inhabiting Euphorbia hirta L. The synthesized nanoparticles were initially confirmed with change in color from the reaction mixture to brown indicating the synthesis of nanoparticles. Further confirmation was achieved with the characteristic absorption peak at 440 nm using UV-Visible spectroscopy. The synthesized silver nanoparticles were subjected to biophysical characterization using hyphenated techniques. The possible role of biomolecules in mediating the synthesis was depicted with FTIR analysis. Further crystalline nature of synthesized nanoparticles was confirmed using X-ray diffraction (XRD) with prominent diffraction peaks at 2θ which can be indexed to the (111), (200), (220), and (311) reflections of face centered cubic structure (fcc) of metallic silver. Transmission electron microscopy (TEM) revealed morphological characteristics of synthesized silver nanoparticles to be polydisperse in nature with size ranging from 10 to 60 nm and different morphological characteristics such as spherical, oval, hexagonal, and cubic shapes. Further silver nanoparticles exhibited bactericidal activity against panel of significant pathogenic bacteria among which Pseudomonas aeruginosa was most sensitive compared to other pathogens. To the best of our knowledge, present study forms first report of bacterial endophyte inhabiting Euphorbia hirta L. in mediating synthesizing silver nanoparticles. Hindawi Publishing Corporation 2016 2016-06-14 /pmc/articles/PMC4923596/ /pubmed/27403378 http://dx.doi.org/10.1155/2016/9020239 Text en Copyright © 2016 Baker Syed et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Syed, Baker Yashavantha Rao, Hoovinakola Chinnappa Nagendra-Prasad, Mysore Nagalingaswamy Prasad, Ashwini Harini, Ballagere Puttaraju Azmath, Pasha Rakshith, Devaraju Satish, Sreedharamurthy Biomimetic Synthesis of Silver Nanoparticles Using Endosymbiotic Bacterium Inhabiting Euphorbia hirta L. and Their Bactericidal Potential |
title | Biomimetic Synthesis of Silver Nanoparticles Using Endosymbiotic Bacterium Inhabiting Euphorbia hirta L. and Their Bactericidal Potential |
title_full | Biomimetic Synthesis of Silver Nanoparticles Using Endosymbiotic Bacterium Inhabiting Euphorbia hirta L. and Their Bactericidal Potential |
title_fullStr | Biomimetic Synthesis of Silver Nanoparticles Using Endosymbiotic Bacterium Inhabiting Euphorbia hirta L. and Their Bactericidal Potential |
title_full_unstemmed | Biomimetic Synthesis of Silver Nanoparticles Using Endosymbiotic Bacterium Inhabiting Euphorbia hirta L. and Their Bactericidal Potential |
title_short | Biomimetic Synthesis of Silver Nanoparticles Using Endosymbiotic Bacterium Inhabiting Euphorbia hirta L. and Their Bactericidal Potential |
title_sort | biomimetic synthesis of silver nanoparticles using endosymbiotic bacterium inhabiting euphorbia hirta l. and their bactericidal potential |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4923596/ https://www.ncbi.nlm.nih.gov/pubmed/27403378 http://dx.doi.org/10.1155/2016/9020239 |
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