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Involvement of the GABAergic system in the neuroprotective and sedative effects of acacetin 7-O-glucoside in rodents

Purpose: Characterization of sedative, possible anticonvulsant, and protective effects of Acacetin-7-O-glucoside (7-ACAG). Methods: 7-ACAG was separated and its purity was analyzed. Its sedative and anti-seizure effects (1, 10, 20, and 40 mg/kg) were evaluated in male mice. Synaptic responses were a...

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Autores principales: Gálvez, Javier, Estrada-Reyes, Rosa, Benítez-King, Gloria, Araujo, Gabriela, Orozco, Sandra, Fernández-Mas, Rodrigo, Almazán, Salvador, Calixto, Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4923766/
https://www.ncbi.nlm.nih.gov/pubmed/26410208
http://dx.doi.org/10.3233/RNN-140486
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author Gálvez, Javier
Estrada-Reyes, Rosa
Benítez-King, Gloria
Araujo, Gabriela
Orozco, Sandra
Fernández-Mas, Rodrigo
Almazán, Salvador
Calixto, Eduardo
author_facet Gálvez, Javier
Estrada-Reyes, Rosa
Benítez-King, Gloria
Araujo, Gabriela
Orozco, Sandra
Fernández-Mas, Rodrigo
Almazán, Salvador
Calixto, Eduardo
author_sort Gálvez, Javier
collection PubMed
description Purpose: Characterization of sedative, possible anticonvulsant, and protective effects of Acacetin-7-O-glucoside (7-ACAG). Methods: 7-ACAG was separated and its purity was analyzed. Its sedative and anti-seizure effects (1, 10, 20, and 40 mg/kg) were evaluated in male mice. Synaptic responses were acquired from area CA1 of hippocampal slices obtained from male Wistar rats. Rats were subjected to stereotaxic surgeries to allow Electroencephalographic (EEG) recordings. Functional recovery was evaluated by measuring the time rats spent in completing the motor task. Then the rats were subjected to right hemiplegia and administered 7-ACAG (40 mg/kg) 1 h or 24 h after surgery. Brains of each group of rats were prepared for histological analysis. Results: Effective sedative doses of 7-ACAG comprised those between 20 and 40 mg/kg. Latency and duration of the epileptiform crisis were delayed by this flavonoid. 7-ACAG decreased the synaptic response in vitro, similar to Gamma-aminobutyric acid (GABA) effects. The flavonoid facilitated functional recovery. This data was associated with preserved cytoarchitecture in brain cortex and hippocampus. Conclusions: 7-ACAG possesses anticonvulsive and sedative effects. Results suggest that GABAergic activity and neuroprotection are involved in the mechanism of action of 7-ACAG and support this compound’s being a potential drug for treatment of anxiety or post-operative conditions caused by neurosurgeries.
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spelling pubmed-49237662016-06-29 Involvement of the GABAergic system in the neuroprotective and sedative effects of acacetin 7-O-glucoside in rodents Gálvez, Javier Estrada-Reyes, Rosa Benítez-King, Gloria Araujo, Gabriela Orozco, Sandra Fernández-Mas, Rodrigo Almazán, Salvador Calixto, Eduardo Restor Neurol Neurosci Research Article Purpose: Characterization of sedative, possible anticonvulsant, and protective effects of Acacetin-7-O-glucoside (7-ACAG). Methods: 7-ACAG was separated and its purity was analyzed. Its sedative and anti-seizure effects (1, 10, 20, and 40 mg/kg) were evaluated in male mice. Synaptic responses were acquired from area CA1 of hippocampal slices obtained from male Wistar rats. Rats were subjected to stereotaxic surgeries to allow Electroencephalographic (EEG) recordings. Functional recovery was evaluated by measuring the time rats spent in completing the motor task. Then the rats were subjected to right hemiplegia and administered 7-ACAG (40 mg/kg) 1 h or 24 h after surgery. Brains of each group of rats were prepared for histological analysis. Results: Effective sedative doses of 7-ACAG comprised those between 20 and 40 mg/kg. Latency and duration of the epileptiform crisis were delayed by this flavonoid. 7-ACAG decreased the synaptic response in vitro, similar to Gamma-aminobutyric acid (GABA) effects. The flavonoid facilitated functional recovery. This data was associated with preserved cytoarchitecture in brain cortex and hippocampus. Conclusions: 7-ACAG possesses anticonvulsive and sedative effects. Results suggest that GABAergic activity and neuroprotection are involved in the mechanism of action of 7-ACAG and support this compound’s being a potential drug for treatment of anxiety or post-operative conditions caused by neurosurgeries. IOS Press 2015-10-05 /pmc/articles/PMC4923766/ /pubmed/26410208 http://dx.doi.org/10.3233/RNN-140486 Text en IOS Press and the authors. All rights reserved https://creativecommons.org/licenses/by-nc/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial (CC BY-NC 4.0) License (https://creativecommons.org/licenses/by-nc/4.0/) , which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gálvez, Javier
Estrada-Reyes, Rosa
Benítez-King, Gloria
Araujo, Gabriela
Orozco, Sandra
Fernández-Mas, Rodrigo
Almazán, Salvador
Calixto, Eduardo
Involvement of the GABAergic system in the neuroprotective and sedative effects of acacetin 7-O-glucoside in rodents
title Involvement of the GABAergic system in the neuroprotective and sedative effects of acacetin 7-O-glucoside in rodents
title_full Involvement of the GABAergic system in the neuroprotective and sedative effects of acacetin 7-O-glucoside in rodents
title_fullStr Involvement of the GABAergic system in the neuroprotective and sedative effects of acacetin 7-O-glucoside in rodents
title_full_unstemmed Involvement of the GABAergic system in the neuroprotective and sedative effects of acacetin 7-O-glucoside in rodents
title_short Involvement of the GABAergic system in the neuroprotective and sedative effects of acacetin 7-O-glucoside in rodents
title_sort involvement of the gabaergic system in the neuroprotective and sedative effects of acacetin 7-o-glucoside in rodents
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4923766/
https://www.ncbi.nlm.nih.gov/pubmed/26410208
http://dx.doi.org/10.3233/RNN-140486
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