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Amelioration of autoimmune arthritis by adoptive transfer of Foxp3-expressing regulatory B cells is associated with the Treg/Th17 cell balance
BACKGROUND: Foxp3 is a key regulator of the development and function of regulatory T cells (Tregs), and its expression is thought to be T cell-restricted. We found that B cells in mice can express Foxp3 and B cells expressing Foxp3 may play a role in preventing the development of collagen-induced ar...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4924280/ https://www.ncbi.nlm.nih.gov/pubmed/27350539 http://dx.doi.org/10.1186/s12967-016-0940-7 |
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author | Park, Mi Kyung Jung, Young Ok Lee, Seon-Yeong Lee, Seung Hoon Heo, Yu Jung Kim, Eun Kyung Oh, Hye Jwa Moon, Young Mee Son, Hye-Jin Park, Min Jung Park, Sung Hwan Kim, Ho Youn La Cho, Mi Min, Jun Ki |
author_facet | Park, Mi Kyung Jung, Young Ok Lee, Seon-Yeong Lee, Seung Hoon Heo, Yu Jung Kim, Eun Kyung Oh, Hye Jwa Moon, Young Mee Son, Hye-Jin Park, Min Jung Park, Sung Hwan Kim, Ho Youn La Cho, Mi Min, Jun Ki |
author_sort | Park, Mi Kyung |
collection | PubMed |
description | BACKGROUND: Foxp3 is a key regulator of the development and function of regulatory T cells (Tregs), and its expression is thought to be T cell-restricted. We found that B cells in mice can express Foxp3 and B cells expressing Foxp3 may play a role in preventing the development of collagen-induced arthritis (CIA) in DBA/1J mice. METHODS: Foxp3 expression was modulated in CD19(+) B cells by transfection with shRNA or using an over-expression construct. In addition, Foxp3-transfected B cells were adoptively transferred to CIA mice. We found that LPS or anti-IgM stimulation induced Foxp3 expression in B cells. Foxp3-expressing B cells were found in the spleens of mice. RESULTS: Over-expression of Foxp3 conferred a contact-dependent suppressive ability on proliferation of responder T cells. Down-regulation of Foxp3 by shRNA caused a profound induction in proliferation of responder T cells. Adoptive transfer of Foxp3(+)CD19(+) B cells attenuated the clinical symptoms of CIA significantly with concomitant suppression of IL-17 production and enhancement of Foxp3 expression in CD4(+) T cells from splenocytes. CONCLUSION: Our data indicate that Foxp3 expression is not restricted to T cells. The expression of Foxp3 in B cells is critical for the immunoregulation of T cells and limits autoimmunity in a mouse model. |
format | Online Article Text |
id | pubmed-4924280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-49242802016-06-29 Amelioration of autoimmune arthritis by adoptive transfer of Foxp3-expressing regulatory B cells is associated with the Treg/Th17 cell balance Park, Mi Kyung Jung, Young Ok Lee, Seon-Yeong Lee, Seung Hoon Heo, Yu Jung Kim, Eun Kyung Oh, Hye Jwa Moon, Young Mee Son, Hye-Jin Park, Min Jung Park, Sung Hwan Kim, Ho Youn La Cho, Mi Min, Jun Ki J Transl Med Research BACKGROUND: Foxp3 is a key regulator of the development and function of regulatory T cells (Tregs), and its expression is thought to be T cell-restricted. We found that B cells in mice can express Foxp3 and B cells expressing Foxp3 may play a role in preventing the development of collagen-induced arthritis (CIA) in DBA/1J mice. METHODS: Foxp3 expression was modulated in CD19(+) B cells by transfection with shRNA or using an over-expression construct. In addition, Foxp3-transfected B cells were adoptively transferred to CIA mice. We found that LPS or anti-IgM stimulation induced Foxp3 expression in B cells. Foxp3-expressing B cells were found in the spleens of mice. RESULTS: Over-expression of Foxp3 conferred a contact-dependent suppressive ability on proliferation of responder T cells. Down-regulation of Foxp3 by shRNA caused a profound induction in proliferation of responder T cells. Adoptive transfer of Foxp3(+)CD19(+) B cells attenuated the clinical symptoms of CIA significantly with concomitant suppression of IL-17 production and enhancement of Foxp3 expression in CD4(+) T cells from splenocytes. CONCLUSION: Our data indicate that Foxp3 expression is not restricted to T cells. The expression of Foxp3 in B cells is critical for the immunoregulation of T cells and limits autoimmunity in a mouse model. BioMed Central 2016-06-28 /pmc/articles/PMC4924280/ /pubmed/27350539 http://dx.doi.org/10.1186/s12967-016-0940-7 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Park, Mi Kyung Jung, Young Ok Lee, Seon-Yeong Lee, Seung Hoon Heo, Yu Jung Kim, Eun Kyung Oh, Hye Jwa Moon, Young Mee Son, Hye-Jin Park, Min Jung Park, Sung Hwan Kim, Ho Youn La Cho, Mi Min, Jun Ki Amelioration of autoimmune arthritis by adoptive transfer of Foxp3-expressing regulatory B cells is associated with the Treg/Th17 cell balance |
title | Amelioration of autoimmune arthritis by adoptive transfer of Foxp3-expressing regulatory B cells is associated with the Treg/Th17 cell balance |
title_full | Amelioration of autoimmune arthritis by adoptive transfer of Foxp3-expressing regulatory B cells is associated with the Treg/Th17 cell balance |
title_fullStr | Amelioration of autoimmune arthritis by adoptive transfer of Foxp3-expressing regulatory B cells is associated with the Treg/Th17 cell balance |
title_full_unstemmed | Amelioration of autoimmune arthritis by adoptive transfer of Foxp3-expressing regulatory B cells is associated with the Treg/Th17 cell balance |
title_short | Amelioration of autoimmune arthritis by adoptive transfer of Foxp3-expressing regulatory B cells is associated with the Treg/Th17 cell balance |
title_sort | amelioration of autoimmune arthritis by adoptive transfer of foxp3-expressing regulatory b cells is associated with the treg/th17 cell balance |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4924280/ https://www.ncbi.nlm.nih.gov/pubmed/27350539 http://dx.doi.org/10.1186/s12967-016-0940-7 |
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