Cargando…

NGS-based deep bisulfite sequencing

We have developed an NGS-based deep bisulfite sequencing protocol for the DNA methylation analysis of genomes. This approach allows the rapid and efficient construction of NGS-ready libraries with a large number of PCR products that have been individually amplified from bisulfite-converted DNA. This...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lee, Suman, Kim, Joomyeong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2015
Materias:	Biochemistry, Genetics and Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4924566/ https://www.ncbi.nlm.nih.gov/pubmed/27366675 http://dx.doi.org/10.1016/j.mex.2015.11.008

Ejemplares similares

A validation of Illumina EPIC array system with bisulfite-based amplicon sequencing
por: Noble, Alexandra J., et al.
Publicado: (2021)

Recovery of bisulfite-converted genomic sequences in the methylation-sensitive QPCR
por: Munson, Kristofer, et al.
Publicado: (2007)

DNA methylation analysis by digital bisulfite genomic sequencing and digital MethyLight
por: Weisenberger, Daniel J., et al.
Publicado: (2008)

Bisulfite probing reveals DNA structural intricacies
por: Bagshaw, Andrew T M, et al.
Publicado: (2023)

RNA sequencing data of mouse 2-cell embryos treated with DMSO
por: Kang, Min-Hee, et al.
Publicado: (2019)

Discovery of RNA secondary structural motifs using sequence-ordered thermodynamic stability and comparative sequence analysis
por: Peterson, Jake M., et al.
Publicado: (2023)

Optimization of Genotype by Sequencing data for phylogenetic purposes
por: Loureiro, L.O., et al.
Publicado: (2020)

Dataset for genome sequencing and de novo assembly of the Vietnamese bighead catfish (Clarias macrocephalus Günther, 1864)
por: Duong, Thuy-Yen, et al.
Publicado: (2020)

Data on the sequence-derived properties of gastric cancer – binding peptides
por: Janairo, Jose Isagani B., et al.
Publicado: (2020)

Isolation of novel sequences targeting highly variable viral protein hemagglutinin()
por: Xu, Zhiwu, et al.
Publicado: (2015)

Complete genome sequence data of a broad-spectrum antipathogen, Bacillus amyloliquefaciens KCTC 18343P, isolated from Makgeolli, Korean traditional rice wine
por: Park, Eun-Hee, et al.
Publicado: (2020)

RNA sequencing data of human prostate cancer cells treated with androgens
por: Poluri, Raghavendra Tejo Karthik, et al.
Publicado: (2019)

Dataset of foraminiferal sedimentary DNA (sedDNA) sequences from Svalbard
por: Pawłowska, Joanna, et al.
Publicado: (2020)

Identifying similarities at metabolic pathways with a strategy of Enzymatic Step Sequences
por: Poot-Hernandez, Augusto Cesar, et al.
Publicado: (2023)

Data for DHK effect on thrombus weight, blood coagulation, blood cell counts and whole blood viscosity in deep vein thrombosis rats
por: Liu, Han, et al.
Publicado: (2019)

RNA sequencing dataset characterizing transcriptomic responses to dietary changes in Caenorhabditis elegans
por: Schumacker, Scott T., et al.
Publicado: (2019)

High-resolution mapping of reciprocal translocation breakpoints using long-read sequencing
por: Chow, Judy F.C., et al.
Publicado: (2019)

Bioinformatic tools for tRNA gene analyses in mitochondrial DNA sequence data
por: Romanova, Elena V., et al.
Publicado: (2020)

18S rDNA sequencing data of benthic polychaetes from the Eastern Arabian Sea
por: Rengaiyan, Periasamy, et al.
Publicado: (2018)

Whole genome sequence data of Lactobacillus fermentum HFD1, the producer of antibacterial peptides
por: Ozhegov, G.D., et al.
Publicado: (2020)

Novel scripts for improved annotation and selection of variants from whole exome sequencing in cancer research
por: Hansen, Marcus Celik, et al.
Publicado: (2015)

RNA sequencing data of human periodontal ligament cells treated with continuous and intermittent compressive force
por: Manokawinchoke, Jeeranan, et al.
Publicado: (2019)

Data relating to sequencing statistics and the reads and genomic coverage aligning to Taenia solium in the cerebrospinal fluid
por: Fei, Xiaofei, et al.
Publicado: (2020)

Draft genome sequence data of Lactobacillus paracasei strain DTA83 isolated from infant stools
por: Lemos Junior, Wilson José Fernandes, et al.
Publicado: (2019)

Sequencing data of cell-free DNA fragments in living-related liver transplantation for inborn errors of metabolism
por: Zhu, Xiaofan, et al.
Publicado: (2020)

Data on degradome sequencing and analysis from mock-inoculated and Fusarium oxysporum treated leaves samples in Persicaria minor
por: Samad, Abdul Fatah A., et al.
Publicado: (2018)

Sequence variation data of the mitochondrial DNA D-loop region of the captive Malayan Gaur (Bos gaurus hubbacki)
por: Md-Zain, Badrul Munir, et al.
Publicado: (2018)

Draft genome sequence data of Rhodosporidium toruloides VN1, a strain capable of producing natural astaxanthin
por: Tran, Tuyet Nhung, et al.
Publicado: (2019)

Draft genome sequence data of T-5 like Salmonella bacteriophage ФSP3 with demonstrated therapeutic potential
por: Sritha, K.S., et al.
Publicado: (2019)

Draft genome sequence data of Microbacterium sp. strain Be9 isolated from uranium-mill tailings porewaters
por: Martínez-Rodríguez, Pablo, et al.
Publicado: (2020)

Data on somatic mutations obtained by whole exome sequencing of FFPE tissue samples from Russian patients with prostate cancer
por: Nikitina, A.S., et al.
Publicado: (2019)

Draft genome sequence data of Paenbacillus curdlanolyticus B-6 possessing a unique xylanolytic-cellulolytic multienzyme system
por: Baramee, Sirilak, et al.
Publicado: (2020)

Genome sequence data of Mangrovimonas sp. strain CR14 isolated from mangrove forest at Tanjung Piai National Park, Malaysia
por: Zakaria, Muhammad Ramziuddin, et al.
Publicado: (2020)

Whole genome sequencing data of Escherichia coli isolated from bloodstream infection patients in Cipto Mangunkusumo National Hospital, Jakarta, Indonesia
por: Nelwan, Erni Juwita, et al.
Publicado: (2020)

Illumina and PacBio DNA sequencing data, de novo assembly and annotation of the genome of Aurantiochytrium limacinum strain CCAP_4062/1
por: Morabito, Christian, et al.
Publicado: (2020)

Molecular screening of Entamoeba spp. (E. histolytica, E. dispar, E. coli, and E. hartmanni) and Giardia intestinalis using PCR and sequencing
por: Tokoro, M., et al.
Publicado: (2023)

Characterization of cell-free breast cancer patient-derived scaffolds using liquid chromatography-mass spectrometry/mass spectrometry data and RNA sequencing data
por: Landberg, Göran, et al.
Publicado: (2020)

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
por: Garrett-Bakelman, Francine E., et al.
Publicado: (2015)

Data on the link between genomic integration of IS1548 and lineage of the strain obtained by bioinformatic analyses of sequenced genomes of Streptococcus agalactiae available at the National Center for Biotechnology Information database
por: Khazaal, Sarah, et al.
Publicado: (2019)

Supergene validation: A model-based protocol for assessing the accuracy of non-model-based supergene methods
por: Adams, Richard H., et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_je9sk4u55iahvc6pod4m4tj0v7