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MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells

LIN28A aberrant expression contributes to the development of human malignancies. However, the LIN28A expression profile remains to be clarified. Herein, we report that LIN28A expression is directly associated with the methylation status of its two CpG island sites in pancreatic cancer cells. First,...

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Autores principales: Xu, Min, Bian, Shihui, Li, Jie, He, Junbo, Chen, Hui, Ge, Lu, Jiao, Zhijun, Zhang, Youli, Peng, Wanxin, Du, Fengyi, Mo, Yinyuan, Gong, Aihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4924729/
https://www.ncbi.nlm.nih.gov/pubmed/26910839
http://dx.doi.org/10.18632/oncotarget.7507
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author Xu, Min
Bian, Shihui
Li, Jie
He, Junbo
Chen, Hui
Ge, Lu
Jiao, Zhijun
Zhang, Youli
Peng, Wanxin
Du, Fengyi
Mo, Yinyuan
Gong, Aihua
author_facet Xu, Min
Bian, Shihui
Li, Jie
He, Junbo
Chen, Hui
Ge, Lu
Jiao, Zhijun
Zhang, Youli
Peng, Wanxin
Du, Fengyi
Mo, Yinyuan
Gong, Aihua
author_sort Xu, Min
collection PubMed
description LIN28A aberrant expression contributes to the development of human malignancies. However, the LIN28A expression profile remains to be clarified. Herein, we report that LIN28A expression is directly associated with the methylation status of its two CpG island sites in pancreatic cancer cells. First, Bisulfite sequencing reveals that PANC1 cells possess the higher methylation rate at LIN28A CpG islands compared with SW1990 and PaTu8988 cells. Subsequently, LIN28A expression is increased at both mRNA and protein levels in pancreatic cancer cells treated with 5-Aza-2′-deoxycytidine (5-Aza-CdR), a DNA methyltransferase inhibitor. Further Chromatin immunoprecipitation (ChIP) assays indicate that methyl-CpG-binding protein 2 (MeCP2) binds preferentially to the two hypermethylated CpG islands sites at LIN28A promoter compare to MBD3. Expectedly, MeCP2 knockdown transcriptionally activates LIN28A expression in above cells, rather than MBD3 knockdown. Moreover, LIN28A overexpression remarkably improves OCT4, NANOG and SOX2 expression, and the ability of sphere and colony formation, and enhances the capacities of invasion in PaTu8988 and SW1990 cells, whereas LIN28A knockdown significantly inhibits the above malignant behaviors in PANC1 cells. These findings suggest that LIN28A is epigenetically regulated via MeCP2 binding to methylated-CpG islands, and may play a crucial role in pancreatic cancer progression.
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spelling pubmed-49247292016-07-13 MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells Xu, Min Bian, Shihui Li, Jie He, Junbo Chen, Hui Ge, Lu Jiao, Zhijun Zhang, Youli Peng, Wanxin Du, Fengyi Mo, Yinyuan Gong, Aihua Oncotarget Research Paper LIN28A aberrant expression contributes to the development of human malignancies. However, the LIN28A expression profile remains to be clarified. Herein, we report that LIN28A expression is directly associated with the methylation status of its two CpG island sites in pancreatic cancer cells. First, Bisulfite sequencing reveals that PANC1 cells possess the higher methylation rate at LIN28A CpG islands compared with SW1990 and PaTu8988 cells. Subsequently, LIN28A expression is increased at both mRNA and protein levels in pancreatic cancer cells treated with 5-Aza-2′-deoxycytidine (5-Aza-CdR), a DNA methyltransferase inhibitor. Further Chromatin immunoprecipitation (ChIP) assays indicate that methyl-CpG-binding protein 2 (MeCP2) binds preferentially to the two hypermethylated CpG islands sites at LIN28A promoter compare to MBD3. Expectedly, MeCP2 knockdown transcriptionally activates LIN28A expression in above cells, rather than MBD3 knockdown. Moreover, LIN28A overexpression remarkably improves OCT4, NANOG and SOX2 expression, and the ability of sphere and colony formation, and enhances the capacities of invasion in PaTu8988 and SW1990 cells, whereas LIN28A knockdown significantly inhibits the above malignant behaviors in PANC1 cells. These findings suggest that LIN28A is epigenetically regulated via MeCP2 binding to methylated-CpG islands, and may play a crucial role in pancreatic cancer progression. Impact Journals LLC 2016-02-19 /pmc/articles/PMC4924729/ /pubmed/26910839 http://dx.doi.org/10.18632/oncotarget.7507 Text en Copyright: © 2016 Xu et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Xu, Min
Bian, Shihui
Li, Jie
He, Junbo
Chen, Hui
Ge, Lu
Jiao, Zhijun
Zhang, Youli
Peng, Wanxin
Du, Fengyi
Mo, Yinyuan
Gong, Aihua
MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells
title MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells
title_full MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells
title_fullStr MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells
title_full_unstemmed MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells
title_short MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells
title_sort mecp2 suppresses lin28a expression via binding to its methylated-cpg islands in pancreatic cancer cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4924729/
https://www.ncbi.nlm.nih.gov/pubmed/26910839
http://dx.doi.org/10.18632/oncotarget.7507
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