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Characterization of Five Podoviridae Phages Infecting Citrobacter freundii

Citrobacter freundii causes opportunistic infections in humans and animals, which are becoming difficult to treat due to increased antibiotic resistance. The aim of this study was to explore phages as potential antimicrobial agents against this opportunistic pathogen. We isolated and characterized f...

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Autores principales: Hamdi, Sana, Rousseau, Geneviève M., Labrie, Simon J., Kourda, Rim S., Tremblay, Denise M., Moineau, Sylvain, Slama, Karim B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4925675/
https://www.ncbi.nlm.nih.gov/pubmed/27446058
http://dx.doi.org/10.3389/fmicb.2016.01023
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author Hamdi, Sana
Rousseau, Geneviève M.
Labrie, Simon J.
Kourda, Rim S.
Tremblay, Denise M.
Moineau, Sylvain
Slama, Karim B.
author_facet Hamdi, Sana
Rousseau, Geneviève M.
Labrie, Simon J.
Kourda, Rim S.
Tremblay, Denise M.
Moineau, Sylvain
Slama, Karim B.
author_sort Hamdi, Sana
collection PubMed
description Citrobacter freundii causes opportunistic infections in humans and animals, which are becoming difficult to treat due to increased antibiotic resistance. The aim of this study was to explore phages as potential antimicrobial agents against this opportunistic pathogen. We isolated and characterized five new virulent phages, SH1, SH2, SH3, SH4, and SH5 from sewage samples in Tunisia. Morphological and genomic analyses revealed that the five C. freundii phages belong to the Caudovirales order, Podoviridae family, and Autographivirinae subfamily. Their linear double-stranded DNA genomes range from 39,158 to 39,832 bp and are terminally redundant with direct repeats between 183 and 242 bp. The five genomes share the same organization as coliphage T7. Based on genomic comparisons and on the phylogeny of the DNA polymerases, we assigned the five phages to the T7virus genus but separated them into two different groups. Phages SH1 and SH2 are very similar to previously characterized phages phiYeO3-12 and phiSG-JL2, infecting, respectively, Yersinia enterocolitica and Salmonella enterica, as well as sharing more than 80% identity with most genes of coliphage T7. Phages SH3, SH4, and SH5 are very similar to phages K1F and Dev2, infecting, respectively, Escherichia coli and Cronobacter turicensis. Several structural proteins of phages SH1, SH3, and SH4 were detected by mass spectrometry. The five phages were also stable from pH 5 to 10. No genes coding for known virulence factors or integrases were found, suggesting that the five isolated phages could be good candidates for therapeutic applications to prevent or treat C. freundii infections. In addition, this study increases our knowledge about the evolutionary relationships within the T7virus genus.
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spelling pubmed-49256752016-07-21 Characterization of Five Podoviridae Phages Infecting Citrobacter freundii Hamdi, Sana Rousseau, Geneviève M. Labrie, Simon J. Kourda, Rim S. Tremblay, Denise M. Moineau, Sylvain Slama, Karim B. Front Microbiol Microbiology Citrobacter freundii causes opportunistic infections in humans and animals, which are becoming difficult to treat due to increased antibiotic resistance. The aim of this study was to explore phages as potential antimicrobial agents against this opportunistic pathogen. We isolated and characterized five new virulent phages, SH1, SH2, SH3, SH4, and SH5 from sewage samples in Tunisia. Morphological and genomic analyses revealed that the five C. freundii phages belong to the Caudovirales order, Podoviridae family, and Autographivirinae subfamily. Their linear double-stranded DNA genomes range from 39,158 to 39,832 bp and are terminally redundant with direct repeats between 183 and 242 bp. The five genomes share the same organization as coliphage T7. Based on genomic comparisons and on the phylogeny of the DNA polymerases, we assigned the five phages to the T7virus genus but separated them into two different groups. Phages SH1 and SH2 are very similar to previously characterized phages phiYeO3-12 and phiSG-JL2, infecting, respectively, Yersinia enterocolitica and Salmonella enterica, as well as sharing more than 80% identity with most genes of coliphage T7. Phages SH3, SH4, and SH5 are very similar to phages K1F and Dev2, infecting, respectively, Escherichia coli and Cronobacter turicensis. Several structural proteins of phages SH1, SH3, and SH4 were detected by mass spectrometry. The five phages were also stable from pH 5 to 10. No genes coding for known virulence factors or integrases were found, suggesting that the five isolated phages could be good candidates for therapeutic applications to prevent or treat C. freundii infections. In addition, this study increases our knowledge about the evolutionary relationships within the T7virus genus. Frontiers Media S.A. 2016-06-29 /pmc/articles/PMC4925675/ /pubmed/27446058 http://dx.doi.org/10.3389/fmicb.2016.01023 Text en Copyright © 2016 Hamdi, Rousseau, Labrie, Kourda, Tremblay, Moineau and Slama. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Hamdi, Sana
Rousseau, Geneviève M.
Labrie, Simon J.
Kourda, Rim S.
Tremblay, Denise M.
Moineau, Sylvain
Slama, Karim B.
Characterization of Five Podoviridae Phages Infecting Citrobacter freundii
title Characterization of Five Podoviridae Phages Infecting Citrobacter freundii
title_full Characterization of Five Podoviridae Phages Infecting Citrobacter freundii
title_fullStr Characterization of Five Podoviridae Phages Infecting Citrobacter freundii
title_full_unstemmed Characterization of Five Podoviridae Phages Infecting Citrobacter freundii
title_short Characterization of Five Podoviridae Phages Infecting Citrobacter freundii
title_sort characterization of five podoviridae phages infecting citrobacter freundii
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4925675/
https://www.ncbi.nlm.nih.gov/pubmed/27446058
http://dx.doi.org/10.3389/fmicb.2016.01023
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