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Seabuckthorn Pulp Oil Protects against Myocardial Ischemia–Reperfusion Injury in Rats through Activation of Akt/eNOS

Seabuckthorn (SBT) pulp oil obtained from the fruits of seabuckthorn [Hippophae rhamnoides L. (Elaeagnaceae)] has been used traditionally for its medicinal and nutritional properties. However, its role in ischemia–reperfusion (IR) injury of myocardium in rats has not been elucidated so far. The pres...

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Autores principales: Suchal, Kapil, Bhatia, Jagriti, Malik, Salma, Malhotra, Rajiv Kumar, Gamad, Nanda, Goyal, Sameer, Nag, Tapas C., Arya, Dharamvir S., Ojha, Shreesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4925700/
https://www.ncbi.nlm.nih.gov/pubmed/27445803
http://dx.doi.org/10.3389/fphar.2016.00155
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author Suchal, Kapil
Bhatia, Jagriti
Malik, Salma
Malhotra, Rajiv Kumar
Gamad, Nanda
Goyal, Sameer
Nag, Tapas C.
Arya, Dharamvir S.
Ojha, Shreesh
author_facet Suchal, Kapil
Bhatia, Jagriti
Malik, Salma
Malhotra, Rajiv Kumar
Gamad, Nanda
Goyal, Sameer
Nag, Tapas C.
Arya, Dharamvir S.
Ojha, Shreesh
author_sort Suchal, Kapil
collection PubMed
description Seabuckthorn (SBT) pulp oil obtained from the fruits of seabuckthorn [Hippophae rhamnoides L. (Elaeagnaceae)] has been used traditionally for its medicinal and nutritional properties. However, its role in ischemia–reperfusion (IR) injury of myocardium in rats has not been elucidated so far. The present study reports the cardioprotective effect of SBT pulp oil in IR-induced model of myocardial infarction in rats and underlying mechanism mediating activation of Akt/eNOS signaling pathway. Male albino Wistar rats were orally administered SBT pulp oil (5, 10, and 20 ml/kg/day) or saline for 30 days. On the day 31, ischemia was induced by one-stage ligation of left anterior descending coronary artery for 45 min followed by reperfusion for 60 min. SBT pulp oil pretreatment at the dose of 20 ml/kg observed to stabilize cardiac function and myocardial antioxidants such as glutathione, superoxide dismutase, catalase, and inhibited lipid peroxidation evidenced by reduced malondialdehyde levels as compared to IR-control group. SBT pulp oil also improved hemodynamic and contractile function and decreased tumor necrosis factor and activities of myocyte injury marker enzymes; lactate dehydrogenase and creatine kinase-MB. Additionally, a remarkable rise in expression of pAkt–eNOS, Bcl-2 and decline in expression of IKKβ/NF-κB and Bax was observed in the myocardium. The histopathological and ultrastructural salvage of cardiomyocytes further supports the cardioprotective effect of SBT pulp oil. Based on findings, it can be concluded that SBT pulp oil protects against myocardial IR injury mediating favorable modulation of Akt-eNOS and IKKβ/NF-κB expression.
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spelling pubmed-49257002016-07-21 Seabuckthorn Pulp Oil Protects against Myocardial Ischemia–Reperfusion Injury in Rats through Activation of Akt/eNOS Suchal, Kapil Bhatia, Jagriti Malik, Salma Malhotra, Rajiv Kumar Gamad, Nanda Goyal, Sameer Nag, Tapas C. Arya, Dharamvir S. Ojha, Shreesh Front Pharmacol Pharmacology Seabuckthorn (SBT) pulp oil obtained from the fruits of seabuckthorn [Hippophae rhamnoides L. (Elaeagnaceae)] has been used traditionally for its medicinal and nutritional properties. However, its role in ischemia–reperfusion (IR) injury of myocardium in rats has not been elucidated so far. The present study reports the cardioprotective effect of SBT pulp oil in IR-induced model of myocardial infarction in rats and underlying mechanism mediating activation of Akt/eNOS signaling pathway. Male albino Wistar rats were orally administered SBT pulp oil (5, 10, and 20 ml/kg/day) or saline for 30 days. On the day 31, ischemia was induced by one-stage ligation of left anterior descending coronary artery for 45 min followed by reperfusion for 60 min. SBT pulp oil pretreatment at the dose of 20 ml/kg observed to stabilize cardiac function and myocardial antioxidants such as glutathione, superoxide dismutase, catalase, and inhibited lipid peroxidation evidenced by reduced malondialdehyde levels as compared to IR-control group. SBT pulp oil also improved hemodynamic and contractile function and decreased tumor necrosis factor and activities of myocyte injury marker enzymes; lactate dehydrogenase and creatine kinase-MB. Additionally, a remarkable rise in expression of pAkt–eNOS, Bcl-2 and decline in expression of IKKβ/NF-κB and Bax was observed in the myocardium. The histopathological and ultrastructural salvage of cardiomyocytes further supports the cardioprotective effect of SBT pulp oil. Based on findings, it can be concluded that SBT pulp oil protects against myocardial IR injury mediating favorable modulation of Akt-eNOS and IKKβ/NF-κB expression. Frontiers Media S.A. 2016-06-29 /pmc/articles/PMC4925700/ /pubmed/27445803 http://dx.doi.org/10.3389/fphar.2016.00155 Text en Copyright © 2016 Suchal, Bhatia, Malik, Malhotra, Gamad, Goyal, Nag, Arya and Ojha. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Suchal, Kapil
Bhatia, Jagriti
Malik, Salma
Malhotra, Rajiv Kumar
Gamad, Nanda
Goyal, Sameer
Nag, Tapas C.
Arya, Dharamvir S.
Ojha, Shreesh
Seabuckthorn Pulp Oil Protects against Myocardial Ischemia–Reperfusion Injury in Rats through Activation of Akt/eNOS
title Seabuckthorn Pulp Oil Protects against Myocardial Ischemia–Reperfusion Injury in Rats through Activation of Akt/eNOS
title_full Seabuckthorn Pulp Oil Protects against Myocardial Ischemia–Reperfusion Injury in Rats through Activation of Akt/eNOS
title_fullStr Seabuckthorn Pulp Oil Protects against Myocardial Ischemia–Reperfusion Injury in Rats through Activation of Akt/eNOS
title_full_unstemmed Seabuckthorn Pulp Oil Protects against Myocardial Ischemia–Reperfusion Injury in Rats through Activation of Akt/eNOS
title_short Seabuckthorn Pulp Oil Protects against Myocardial Ischemia–Reperfusion Injury in Rats through Activation of Akt/eNOS
title_sort seabuckthorn pulp oil protects against myocardial ischemia–reperfusion injury in rats through activation of akt/enos
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4925700/
https://www.ncbi.nlm.nih.gov/pubmed/27445803
http://dx.doi.org/10.3389/fphar.2016.00155
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