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The Value of Cytology Smears for Acanthamoeba Keratitis

Purpose. Acanthamoeba keratitis remains a difficult diagnosis despite advances in genetic and imaging technologies. The purpose of this paper is to highlight the utility of cytology smears for diagnosis of Acanthamoeba keratitis. Methods. This is a case study of the diagnostic course for a patient w...

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Autores principales: Patel, Sangita P., Schaefer, Jamie L., Jaber, Ryan, Paterson, Joyce, Liu, Weiguo, Gonzalez-Fernandez, Federico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4925955/
https://www.ncbi.nlm.nih.gov/pubmed/27403362
http://dx.doi.org/10.1155/2016/4148968
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author Patel, Sangita P.
Schaefer, Jamie L.
Jaber, Ryan
Paterson, Joyce
Liu, Weiguo
Gonzalez-Fernandez, Federico
author_facet Patel, Sangita P.
Schaefer, Jamie L.
Jaber, Ryan
Paterson, Joyce
Liu, Weiguo
Gonzalez-Fernandez, Federico
author_sort Patel, Sangita P.
collection PubMed
description Purpose. Acanthamoeba keratitis remains a difficult diagnosis despite advances in genetic and imaging technologies. The purpose of this paper is to highlight the utility of cytology smears for diagnosis of Acanthamoeba keratitis. Methods. This is a case study of the diagnostic course for a patient with suspected Acanthamoeba keratitis. Results. A 40-year-old male with poor contact lens hygiene presented with severe left eye pain. Slit lamp examination showed two peripheral ring infiltrates without an epithelial defect. The epithelium over both infiltrates was removed with a Kimura spatula. Half of the sample was smeared on a dry microscope slide and the other half was submitted for Acanthamoeba culture and PCR. Both culture and PCR were negative for Acanthamoeba, but hematoxylin and eosin stain of the smear revealed double-walled cysts. Conclusion. H&E staining of corneal cytology specimens is an efficient and readily available test for diagnosis of Acanthamoeba keratitis.
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spelling pubmed-49259552016-07-11 The Value of Cytology Smears for Acanthamoeba Keratitis Patel, Sangita P. Schaefer, Jamie L. Jaber, Ryan Paterson, Joyce Liu, Weiguo Gonzalez-Fernandez, Federico Case Rep Ophthalmol Med Case Report Purpose. Acanthamoeba keratitis remains a difficult diagnosis despite advances in genetic and imaging technologies. The purpose of this paper is to highlight the utility of cytology smears for diagnosis of Acanthamoeba keratitis. Methods. This is a case study of the diagnostic course for a patient with suspected Acanthamoeba keratitis. Results. A 40-year-old male with poor contact lens hygiene presented with severe left eye pain. Slit lamp examination showed two peripheral ring infiltrates without an epithelial defect. The epithelium over both infiltrates was removed with a Kimura spatula. Half of the sample was smeared on a dry microscope slide and the other half was submitted for Acanthamoeba culture and PCR. Both culture and PCR were negative for Acanthamoeba, but hematoxylin and eosin stain of the smear revealed double-walled cysts. Conclusion. H&E staining of corneal cytology specimens is an efficient and readily available test for diagnosis of Acanthamoeba keratitis. Hindawi Publishing Corporation 2016 2016-06-15 /pmc/articles/PMC4925955/ /pubmed/27403362 http://dx.doi.org/10.1155/2016/4148968 Text en Copyright © 2016 Sangita P. Patel et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Case Report
Patel, Sangita P.
Schaefer, Jamie L.
Jaber, Ryan
Paterson, Joyce
Liu, Weiguo
Gonzalez-Fernandez, Federico
The Value of Cytology Smears for Acanthamoeba Keratitis
title The Value of Cytology Smears for Acanthamoeba Keratitis
title_full The Value of Cytology Smears for Acanthamoeba Keratitis
title_fullStr The Value of Cytology Smears for Acanthamoeba Keratitis
title_full_unstemmed The Value of Cytology Smears for Acanthamoeba Keratitis
title_short The Value of Cytology Smears for Acanthamoeba Keratitis
title_sort value of cytology smears for acanthamoeba keratitis
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4925955/
https://www.ncbi.nlm.nih.gov/pubmed/27403362
http://dx.doi.org/10.1155/2016/4148968
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