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Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India
This study is to probe the pattern of antibiotic resistance against aminoglycosides and its mechanism in E. coli obtained from patients from Chennai, India. Isolation and identification of pathogens were done on MacConkey agar. Antimicrobial sensitivity testing was done by disc diffusion test. The i...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926017/ https://www.ncbi.nlm.nih.gov/pubmed/27403451 http://dx.doi.org/10.1155/2016/1875865 |
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author | Mir, Abdul Rouf Bashir, Yasir Dar, Firdous Ahmad Sekhar, M. |
author_facet | Mir, Abdul Rouf Bashir, Yasir Dar, Firdous Ahmad Sekhar, M. |
author_sort | Mir, Abdul Rouf |
collection | PubMed |
description | This study is to probe the pattern of antibiotic resistance against aminoglycosides and its mechanism in E. coli obtained from patients from Chennai, India. Isolation and identification of pathogens were done on MacConkey agar. Antimicrobial sensitivity testing was done by disc diffusion test. The identification of genes encoding aminoglycoside modifying enzymes was done by Polymerase Chain Reaction (PCR). Out of 98 isolates, 71 (72.45%) isolates were identified as E. coli and the remaining 27 (27.55%) as other bacteria. Disc diffusion method results showed a resistance level of 72.15% for streptomycin, 73.4% for gentamicin, 63.26% for neomycin, 57.14% for tobramycin, 47.9% for netilmicin, and 8.16% for amikacin in E. coli. PCR screening showed the presence of four genes, namely, rrs, aacC2, aacA-aphD, and aphA3, in their plasmid DNA. The results point towards the novel mechanism of drug resistance in E. coli from UTI patients in India as they confirm the presence of genes encoding enzymes that cause resistance to aminoglycoside drugs. This could be an alarm for drug prescription to UTI patients. |
format | Online Article Text |
id | pubmed-4926017 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-49260172016-07-11 Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India Mir, Abdul Rouf Bashir, Yasir Dar, Firdous Ahmad Sekhar, M. ScientificWorldJournal Research Article This study is to probe the pattern of antibiotic resistance against aminoglycosides and its mechanism in E. coli obtained from patients from Chennai, India. Isolation and identification of pathogens were done on MacConkey agar. Antimicrobial sensitivity testing was done by disc diffusion test. The identification of genes encoding aminoglycoside modifying enzymes was done by Polymerase Chain Reaction (PCR). Out of 98 isolates, 71 (72.45%) isolates were identified as E. coli and the remaining 27 (27.55%) as other bacteria. Disc diffusion method results showed a resistance level of 72.15% for streptomycin, 73.4% for gentamicin, 63.26% for neomycin, 57.14% for tobramycin, 47.9% for netilmicin, and 8.16% for amikacin in E. coli. PCR screening showed the presence of four genes, namely, rrs, aacC2, aacA-aphD, and aphA3, in their plasmid DNA. The results point towards the novel mechanism of drug resistance in E. coli from UTI patients in India as they confirm the presence of genes encoding enzymes that cause resistance to aminoglycoside drugs. This could be an alarm for drug prescription to UTI patients. Hindawi Publishing Corporation 2016 2016-06-15 /pmc/articles/PMC4926017/ /pubmed/27403451 http://dx.doi.org/10.1155/2016/1875865 Text en Copyright © 2016 Abdul Rouf Mir et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Mir, Abdul Rouf Bashir, Yasir Dar, Firdous Ahmad Sekhar, M. Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India |
title | Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India |
title_full | Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India |
title_fullStr | Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India |
title_full_unstemmed | Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India |
title_short | Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India |
title_sort | identification of genes coding aminoglycoside modifying enzymes in e. coli of uti patients in india |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926017/ https://www.ncbi.nlm.nih.gov/pubmed/27403451 http://dx.doi.org/10.1155/2016/1875865 |
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