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Expression and purification of the modification-dependent restriction enzyme BisI and its homologous enzymes

The methylation-dependent restriction endonuclease (REase) BisI (G(m5)C ↓ NGC) is found in Bacillus subtilis T30. We expressed and purified the BisI endonuclease and 34 BisI homologs identified in bacterial genomes. 23 of these BisI homologs are active based on digestion of (m5)C-modified substrates...

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Detalles Bibliográficos
Autores principales:	Xu, Shuang-yong, Klein, Pernelle, Degtyarev, Sergey Kh., Roberts, Richard J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926085/ https://www.ncbi.nlm.nih.gov/pubmed/27353146 http://dx.doi.org/10.1038/srep28579

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