Recombinant Sheep Pox Virus Proteins Elicit Neutralizing Antibodies

The aim of this work was to evaluate the immunogenicity and neutralizing activity of sheep pox virus (SPPV; genus Capripoxvirus, family Poxviridae) structural proteins as candidate subunit vaccines to control sheep pox disease. SPPV structural proteins were identified by sequence homology with prote...

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Autores principales: Chervyakova, Olga V., Zaitsev, Valentin L., Iskakov, Bulat K., Tailakova, Elmira T., Strochkov, Vitaliy M., Sultankulova, Kulyaisan T., Sandybayev, Nurlan T., Stanbekova, Gulshan E., Beisenov, Daniyar K., Abduraimov, Yergali O., Mambetaliyev, Muratbay, Sansyzbay, Abylay R., Kovalskaya, Natalia Y., Nemchinov, Lev. G., Hammond, Rosemarie W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926179/
https://www.ncbi.nlm.nih.gov/pubmed/27338444
http://dx.doi.org/10.3390/v8060159
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author Chervyakova, Olga V.
Zaitsev, Valentin L.
Iskakov, Bulat K.
Tailakova, Elmira T.
Strochkov, Vitaliy M.
Sultankulova, Kulyaisan T.
Sandybayev, Nurlan T.
Stanbekova, Gulshan E.
Beisenov, Daniyar K.
Abduraimov, Yergali O.
Mambetaliyev, Muratbay
Sansyzbay, Abylay R.
Kovalskaya, Natalia Y.
Nemchinov, Lev. G.
Hammond, Rosemarie W.
author_facet Chervyakova, Olga V.
Zaitsev, Valentin L.
Iskakov, Bulat K.
Tailakova, Elmira T.
Strochkov, Vitaliy M.
Sultankulova, Kulyaisan T.
Sandybayev, Nurlan T.
Stanbekova, Gulshan E.
Beisenov, Daniyar K.
Abduraimov, Yergali O.
Mambetaliyev, Muratbay
Sansyzbay, Abylay R.
Kovalskaya, Natalia Y.
Nemchinov, Lev. G.
Hammond, Rosemarie W.
author_sort Chervyakova, Olga V.
collection PubMed
description The aim of this work was to evaluate the immunogenicity and neutralizing activity of sheep pox virus (SPPV; genus Capripoxvirus, family Poxviridae) structural proteins as candidate subunit vaccines to control sheep pox disease. SPPV structural proteins were identified by sequence homology with proteins of vaccinia virus (VACV) strain Copenhagen. Four SPPV proteins (SPPV-ORF 060, SPPV-ORF 095, SPPV-ORF 117, and SPPV-ORF 122), orthologs of immunodominant L1, A4, A27, and A33 VACV proteins, respectively, were produced in Escherichia coli. Western blot analysis revealed the antigenic and immunogenic properties of SPPV-060, SPPV-095, SPPV-117 and SPPV-122 proteins when injected with adjuvant into experimental rabbits. Virus-neutralizing activity against SPPV in lamb kidney cell culture was detected for polyclonal antisera raised to SPPV-060, SPPV-117, and SPPV-122 proteins. To our knowledge, this is the first report demonstrating the virus-neutralizing activities of antisera raised to SPPV-060, SPPV-117, and SPPV-122 proteins.
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spelling pubmed-49261792016-07-06 Recombinant Sheep Pox Virus Proteins Elicit Neutralizing Antibodies Chervyakova, Olga V. Zaitsev, Valentin L. Iskakov, Bulat K. Tailakova, Elmira T. Strochkov, Vitaliy M. Sultankulova, Kulyaisan T. Sandybayev, Nurlan T. Stanbekova, Gulshan E. Beisenov, Daniyar K. Abduraimov, Yergali O. Mambetaliyev, Muratbay Sansyzbay, Abylay R. Kovalskaya, Natalia Y. Nemchinov, Lev. G. Hammond, Rosemarie W. Viruses Communication The aim of this work was to evaluate the immunogenicity and neutralizing activity of sheep pox virus (SPPV; genus Capripoxvirus, family Poxviridae) structural proteins as candidate subunit vaccines to control sheep pox disease. SPPV structural proteins were identified by sequence homology with proteins of vaccinia virus (VACV) strain Copenhagen. Four SPPV proteins (SPPV-ORF 060, SPPV-ORF 095, SPPV-ORF 117, and SPPV-ORF 122), orthologs of immunodominant L1, A4, A27, and A33 VACV proteins, respectively, were produced in Escherichia coli. Western blot analysis revealed the antigenic and immunogenic properties of SPPV-060, SPPV-095, SPPV-117 and SPPV-122 proteins when injected with adjuvant into experimental rabbits. Virus-neutralizing activity against SPPV in lamb kidney cell culture was detected for polyclonal antisera raised to SPPV-060, SPPV-117, and SPPV-122 proteins. To our knowledge, this is the first report demonstrating the virus-neutralizing activities of antisera raised to SPPV-060, SPPV-117, and SPPV-122 proteins. MDPI 2016-06-07 /pmc/articles/PMC4926179/ /pubmed/27338444 http://dx.doi.org/10.3390/v8060159 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Chervyakova, Olga V.
Zaitsev, Valentin L.
Iskakov, Bulat K.
Tailakova, Elmira T.
Strochkov, Vitaliy M.
Sultankulova, Kulyaisan T.
Sandybayev, Nurlan T.
Stanbekova, Gulshan E.
Beisenov, Daniyar K.
Abduraimov, Yergali O.
Mambetaliyev, Muratbay
Sansyzbay, Abylay R.
Kovalskaya, Natalia Y.
Nemchinov, Lev. G.
Hammond, Rosemarie W.
Recombinant Sheep Pox Virus Proteins Elicit Neutralizing Antibodies
title Recombinant Sheep Pox Virus Proteins Elicit Neutralizing Antibodies
title_full Recombinant Sheep Pox Virus Proteins Elicit Neutralizing Antibodies
title_fullStr Recombinant Sheep Pox Virus Proteins Elicit Neutralizing Antibodies
title_full_unstemmed Recombinant Sheep Pox Virus Proteins Elicit Neutralizing Antibodies
title_short Recombinant Sheep Pox Virus Proteins Elicit Neutralizing Antibodies
title_sort recombinant sheep pox virus proteins elicit neutralizing antibodies
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926179/
https://www.ncbi.nlm.nih.gov/pubmed/27338444
http://dx.doi.org/10.3390/v8060159
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