Biophysical constraints of optogenetic inhibition at presynaptic terminals

We investigated the efficacy of optogenetic inhibition at presynaptic terminals using halorhodopsin, archaerhodopsin and chloride-conducting channelrhodopsins. Precisely timed activation of both archaerhodopsin and halorhodpsin at presynaptic terminals attenuated evoked release. However, sustained a...

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Autores principales: Mahn, Mathias, Prigge, Matthias, Ron, Shiri, Levy, Rivka, Yizhar, Ofer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926958/
https://www.ncbi.nlm.nih.gov/pubmed/26950004
http://dx.doi.org/10.1038/nn.4266
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author Mahn, Mathias
Prigge, Matthias
Ron, Shiri
Levy, Rivka
Yizhar, Ofer
author_facet Mahn, Mathias
Prigge, Matthias
Ron, Shiri
Levy, Rivka
Yizhar, Ofer
author_sort Mahn, Mathias
collection PubMed
description We investigated the efficacy of optogenetic inhibition at presynaptic terminals using halorhodopsin, archaerhodopsin and chloride-conducting channelrhodopsins. Precisely timed activation of both archaerhodopsin and halorhodpsin at presynaptic terminals attenuated evoked release. However, sustained archaerhodopsin activation was paradoxically associated with increased spontaneous release. Activation of chloride-conducting channelrhodopsins triggered neurotransmitter release upon light onset. Our results indicate that the biophysical properties of presynaptic terminals dictate unique boundary conditions for optogenetic manipulation.
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spelling pubmed-49269582016-09-22 Biophysical constraints of optogenetic inhibition at presynaptic terminals Mahn, Mathias Prigge, Matthias Ron, Shiri Levy, Rivka Yizhar, Ofer Nat Neurosci Article We investigated the efficacy of optogenetic inhibition at presynaptic terminals using halorhodopsin, archaerhodopsin and chloride-conducting channelrhodopsins. Precisely timed activation of both archaerhodopsin and halorhodpsin at presynaptic terminals attenuated evoked release. However, sustained archaerhodopsin activation was paradoxically associated with increased spontaneous release. Activation of chloride-conducting channelrhodopsins triggered neurotransmitter release upon light onset. Our results indicate that the biophysical properties of presynaptic terminals dictate unique boundary conditions for optogenetic manipulation. 2016-03-07 2016-04 /pmc/articles/PMC4926958/ /pubmed/26950004 http://dx.doi.org/10.1038/nn.4266 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Mahn, Mathias
Prigge, Matthias
Ron, Shiri
Levy, Rivka
Yizhar, Ofer
Biophysical constraints of optogenetic inhibition at presynaptic terminals
title Biophysical constraints of optogenetic inhibition at presynaptic terminals
title_full Biophysical constraints of optogenetic inhibition at presynaptic terminals
title_fullStr Biophysical constraints of optogenetic inhibition at presynaptic terminals
title_full_unstemmed Biophysical constraints of optogenetic inhibition at presynaptic terminals
title_short Biophysical constraints of optogenetic inhibition at presynaptic terminals
title_sort biophysical constraints of optogenetic inhibition at presynaptic terminals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926958/
https://www.ncbi.nlm.nih.gov/pubmed/26950004
http://dx.doi.org/10.1038/nn.4266
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AT levyrivka biophysicalconstraintsofoptogeneticinhibitionatpresynapticterminals
AT yizharofer biophysicalconstraintsofoptogeneticinhibitionatpresynapticterminals

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