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Linking Genomo- and Pathotype: Exploiting the Zebrafish Embryo Model to Investigate the Divergent Virulence Potential among Cronobacter spp.

Bacteria belonging to the genus Cronobacter have been recognized as causative agents of life-threatening systemic infections primarily in premature, low-birth weight and immune-compromised neonates. Apparently not all Cronobacter species are linked to infantile infections and it has been proposed th...

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Autores principales: Eshwar, Athmanya K., Tall, Ben D., Gangiredla, Jayanthi, Gopinath, Gopal R., Patel, Isha R., Neuhauss, Stephan C. F., Stephan, Roger, Lehner, Angelika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927158/
https://www.ncbi.nlm.nih.gov/pubmed/27355472
http://dx.doi.org/10.1371/journal.pone.0158428
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author Eshwar, Athmanya K.
Tall, Ben D.
Gangiredla, Jayanthi
Gopinath, Gopal R.
Patel, Isha R.
Neuhauss, Stephan C. F.
Stephan, Roger
Lehner, Angelika
author_facet Eshwar, Athmanya K.
Tall, Ben D.
Gangiredla, Jayanthi
Gopinath, Gopal R.
Patel, Isha R.
Neuhauss, Stephan C. F.
Stephan, Roger
Lehner, Angelika
author_sort Eshwar, Athmanya K.
collection PubMed
description Bacteria belonging to the genus Cronobacter have been recognized as causative agents of life-threatening systemic infections primarily in premature, low-birth weight and immune-compromised neonates. Apparently not all Cronobacter species are linked to infantile infections and it has been proposed that virulence varies among strains. Whole genome comparisons and in silico analysis have proven to be powerful tools in elucidating potential virulence determinants, the presence/absence of which may explain the differential virulence behaviour of strains. However, validation of these factors has in the past been hampered by the availability of a suitable neonatal animal model. In the present study we have used zebrafish embryos to model Cronobacter infections in vivo using wild type and genetically engineered strains. Our experiments confirmed the role of the RepF1B-like plasmids as “virulence plasmids” in Cronobacter and underpinned the importantce of two putative virulence factors—cpa and zpx—in in vivo pathogenesis. We propose that by using this model in vivo infection studies are now possible on a large scale level which will boost the understanding on the virulence strategies employed by these pathogens.
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spelling pubmed-49271582016-07-18 Linking Genomo- and Pathotype: Exploiting the Zebrafish Embryo Model to Investigate the Divergent Virulence Potential among Cronobacter spp. Eshwar, Athmanya K. Tall, Ben D. Gangiredla, Jayanthi Gopinath, Gopal R. Patel, Isha R. Neuhauss, Stephan C. F. Stephan, Roger Lehner, Angelika PLoS One Research Article Bacteria belonging to the genus Cronobacter have been recognized as causative agents of life-threatening systemic infections primarily in premature, low-birth weight and immune-compromised neonates. Apparently not all Cronobacter species are linked to infantile infections and it has been proposed that virulence varies among strains. Whole genome comparisons and in silico analysis have proven to be powerful tools in elucidating potential virulence determinants, the presence/absence of which may explain the differential virulence behaviour of strains. However, validation of these factors has in the past been hampered by the availability of a suitable neonatal animal model. In the present study we have used zebrafish embryos to model Cronobacter infections in vivo using wild type and genetically engineered strains. Our experiments confirmed the role of the RepF1B-like plasmids as “virulence plasmids” in Cronobacter and underpinned the importantce of two putative virulence factors—cpa and zpx—in in vivo pathogenesis. We propose that by using this model in vivo infection studies are now possible on a large scale level which will boost the understanding on the virulence strategies employed by these pathogens. Public Library of Science 2016-06-29 /pmc/articles/PMC4927158/ /pubmed/27355472 http://dx.doi.org/10.1371/journal.pone.0158428 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Eshwar, Athmanya K.
Tall, Ben D.
Gangiredla, Jayanthi
Gopinath, Gopal R.
Patel, Isha R.
Neuhauss, Stephan C. F.
Stephan, Roger
Lehner, Angelika
Linking Genomo- and Pathotype: Exploiting the Zebrafish Embryo Model to Investigate the Divergent Virulence Potential among Cronobacter spp.
title Linking Genomo- and Pathotype: Exploiting the Zebrafish Embryo Model to Investigate the Divergent Virulence Potential among Cronobacter spp.
title_full Linking Genomo- and Pathotype: Exploiting the Zebrafish Embryo Model to Investigate the Divergent Virulence Potential among Cronobacter spp.
title_fullStr Linking Genomo- and Pathotype: Exploiting the Zebrafish Embryo Model to Investigate the Divergent Virulence Potential among Cronobacter spp.
title_full_unstemmed Linking Genomo- and Pathotype: Exploiting the Zebrafish Embryo Model to Investigate the Divergent Virulence Potential among Cronobacter spp.
title_short Linking Genomo- and Pathotype: Exploiting the Zebrafish Embryo Model to Investigate the Divergent Virulence Potential among Cronobacter spp.
title_sort linking genomo- and pathotype: exploiting the zebrafish embryo model to investigate the divergent virulence potential among cronobacter spp.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927158/
https://www.ncbi.nlm.nih.gov/pubmed/27355472
http://dx.doi.org/10.1371/journal.pone.0158428
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